The experimental study of inhibition of effective constituent of Giant Typhonium Rhizome on human bone marrow neuroblastoma cell (SH-SY5Y) proilferation / 国际中医中药杂志

ObjectiveTo investigate the anti tumor effect of Giant Typhonium Rhizome.Methods SH-SY5Y was treated for 24 or 48 h by Giant Typhonium Rhizome.Cell Counting Kit-8 (CCK-8) cell proliferation and cytotoxicity assays were applied to detect the inhibition of SH-SY5Y cells treated with aqueous extract of white monkshood root.AV/PI double staining flow cytometry was applied to explore the mechanism of growth inhibition.ResultsSH-SY5Y cells could be inhibited by root aqueous extract of Giant Typhonium Rhizome,the IC50 at 48 h (50％ inhibitory concentration) was 0.862 mg/ml,while the IC50 at 24 h was 1.158 mg/ml.Western-blot results showed that root aqueous extract of Giant Typhonium Rhizome could induce apoptosis to inhibit tumor cells.ConclusionThe Rhizoma typhonii inhibit the growth of SH-SY5Y.

Reactive oxygen species(ROS) involving in inhibiting proliferation of human bone marrow neuroblastoma cells (SH-SY5Y) by giant typhonium rhizome water extract / 国际中医中药杂志

ObjectiveTo investigate whether the reactive oxygen species involved in inhibiting proliferation of human bone marrow cells of neuroblastoma cells (SH-SY5Y)process by giant typhonium rhizome water extract,and to investigate the initial mechanism of anti-tumor effect of giant typhonium rhizome in vitro.MethodsAfter giant typhonium rhizome water extract processing of human bone marrow neuroblastoma cells (SH-SY5Y)at 24 h and 48 h,the cells were collected,and western blotting reaction methods such as flow cytometry was used to study the levels of ROS and SH-SY5Y apoptosis.ResultsThe levels of ROS elevated from before dosing (4.9±1.6)％ to (39.7 ± 11.8)％ in the process of giant typhonium rhizome water extract induced apoptosis in SH-SY5Y; after ROS inhibition was added,SH-SY5Y apoptosis level induced by giant typhonium rhizome water extract had decreased.ConclusionROS involved in the process of giant typhonium rhizome water extract inhibiting cell proliferation in SH-SY5Y.

Effect of Three Kinds of Polysaccharides from Herbal Medicines on Immunomodulation in Mice / 中国中医药信息杂志

Objective To explore the features of immune responsed activated by polysaccharides from herbal medicines and provide basis for immunomodulation of polysaccharides. Methods The mice were immuned by polysaccharides of Angelica, Atractylodes and Giant typhonium rhizome respectively, then the corresponeding antibodies and cross-reactive antibodies in serum were detected by ELISA. Results Three kinds of polysaccharides from herbal medicines could all activated the mice to produce not only the corresponding antibodies, but also the cross-reactive antibodies, the ranges of non-specific antibodies activated by distinct polysaccharide were different. Conclusions Polysaccharides from herbal medicines may be a kind of non-specific and broad-spectrum immunomodulators.