Validating the physiological potential of zero monopodial compact cotton TCH 1819 culture by chemical manipulation

Cotton is the most important global cash crop which controls economy of many nations. Global sustainability of cotton yield is one of the major challenges for meeting impending threats under climate change. Though India is one among the leading countries in cotton production, the supply is not enough considering the increasing demand. Scientists across the Globe are indulged in developing new lines and cultures with capacity to produce more yields. In this context, here, we have made an attempt to study the growth, physiology, and yield traits of cotton culture - TCH 1819 before its release (now released in the name of CO 17) by different chemical treatments. Observation on the leaf gas exchange traits, leaf parenchymal cells distinguished the source sink relationship of the culture. Chemical manipulation by growth retardants reduced the gibberellins content and modified the foliage structure. By characterizing the physiological potential through manipulation by growth retardant (Mepiquat chloride (0.015 %)) increased the yield by 30%. The traits identified in this study are potential indicators in breeding programme before releasing the variety.

JcZFP8, a C2H2 zinc finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco

Background: Jatropha curcas L., as an important strategic biofuel resource with considerable economic potential, has attracted worldwide attention. However, J. curcas has yet to be domesticated. Plant height, an important agronomic trait of J. curcas, has not been sufficiently improved, and the genetic regulation of this trait in J. curcas is not fully understood. Zinc finger proteins (ZFPs), a class of transcription factors, have previously been shown to play critical roles in regulating multiple aspects of plant growth and development and may accordingly be implicated in the genetic regulation of plant height in J. curcas. Results: In this study, we cloned JcZFP8, a C2H2 ZFP gene in J. curcas. We found that the JcZFP8 protein was localized in the nucleus and contained a conserved QALGGH motif in its C2H2 structure. Furthermore, ectopic expression of JcZFP8 under the control of the 35S promoter in transgenic tobacco resulted in dwarf plants with malformed leaves. However, when JcZFP8 was knocked out, the transgenic tobacco did not show the dwarf phenotype. After treatment with the gibberellic acid (GA) biosynthesis inhibitor paclobutrazol (PAC), the dwarf phenotype was more severe than plants that did not receive the PAC treatment, whereas application of exogenous gibberellin3 (GA3) reduced the dwarf phenotype in transgenic plants. Conclusions: The results of this study indicate that JcZFP8 may play a role in J. curcas plant phenotype through GA-related pathways. Our findings may help us to understand the genetic regulation of plant development in J. curcas and to accelerate breeding progress through engineering of the GA metabolic pathway in this plant. How to cite: Shi X,Wu Y, Dai T, et al. JcZFP8, a C2H2 zinc-finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco.

Transcriptional activity and subcellular location of SmWRKY42-like and its response to gibberellin and ethylene treatments in Salvia miltiorrhiza hairy roots / 中草药·英文版

Objective: The exogenous gibberellin (GA) and ethylene (ET) treatment can improve the medicinal ingredients of Salvia miltiorrhiza. Interestingly, many reports pointed out that WRKY transcription factors played an important regulatory role in these treatment responses. However, whether the SmWRKY mediate these treatment signalings in S. miltiorrhiza remains largely elusive. Methods: qRT-PCR was used for SmWRKY42-like in response to exogenous GA and ethephon (Eth) treatment. The subcellular location of SmWRKY42-like was transiently transformed into onion epidermal cells by particle bombardment. The self-activating activity of SmWRKY42-like was verified in AH109 yeast strain. Results: SmWRKY42-like was a WRKY family gene in S. miltiorrhiza. The subcellular localization and transcriptional activity results of the SmWRKY42-like protein indicated that SmWRKY42-like mainly enriched in nucleus and might be a transcription factor in S. miltiorrhiza. In the meantime, the SmWRKY42-like gene significantly responded to exogenous GA and Eth treatment. Conclusion: These results collectively indicated the SmWRKY42-like gene functions, as an important hormone-responsive gene, might play a potentially role in ET and GA signaling pathways.

Efecto de las hormonas vegetales y el fotoperiodo en la producción de microtubérculos de papa (Solanum tuberosum L.) / Effect of plant hormones and photoperiod on the production of potato microtubers (Solanum tuberosum L.)

RESUMEN Existen numerosos factores que afectan la micropropagación y la microtuberización en papa; entre ellos, las hormonas vegetales y el fotoperiodo. Para estudiar el efecto de estos dos factores en las variedades 'Arbolona negra' (AN) y 'Granola' (G), se cultivaron microesquejes de cada variedad en medio MS líquido con o sin giberelinas (GA) y con 25 g/L de sacarosa e incubados bajo condiciones de luz blanca continua. Para inducir la microtuberización, las vitroplántulas obtenidas fueron sub-cultivadas en medio MS suplementado con 50 g/L sacarosa, tres concentraciones de BA (0, 1 y 5 mg/L) e incubadas bajo diferentes regímenes lumínicos. El pre-tratamiento con GA favoreció el alargamiento del vástago en AN pero no en G. Ambas variedades produjeron el mayor número de microtubérculos en medio MS suplementado con 5 mg/L de BA, bajo condiciones fotoperiódicas, sin la adición previa de GA. El cultivo in vitro de microesquejes de papa en medios de cultivo suplementados con BA y sacarosa, y la incubación bajo condiciones de días cortos permite obtener microtubérculos de papa en condiciones in vitro, en un tiempo más corto que el que podría esperarse en condiciones tradicionales de cultivo.

ABSTRACT Micropropagation and microtuberization in potato plants are both affected by plant hormones and photoperiod. To study the effect of these factors on potato cultivars 'Arbolona negra' (AN) and 'Granola' (G), microcuttings of each cultivar were cultured on MS liquid medium supplemented with sucrose 25 g/L, with or without gibberellins (GA). These microcuttings were incubated under continuous light. In order to induce microtuberization, the obtained vitroplantlets were subcultured on MS medium supplemented with sucrose 50 g/L, three concentrations of BA (0, 1 and 5 mg/L) and incubated under different light patterns. GA pre-treatment induced shoots elongation on AN but not on G cultivar. The highest microtubers production for both cultivars was achieved on MS medium supplemented with BA 5 mg/L under short day light condition without GA pretreatment. The in vitro culture of microcuttings on culture media supplemented with BA and sucrose, incubated under short day conditions, allowed microtubers production in a shorter time lapse.

Molecular cloning and sequence analysis of two GA2ox genes in Pseudostellariae Radix / 中草药

Objective: To clone the gibberellin 2-oxidase (GA2ox) genes from Pseudostellaria heterophylla and perform the bioinformatic and expression mode analysis. Methods: According to P. heterophylla transcriptome annotation, two transcript codings of GA2ox were identified. The full-length cDNA PhGA2ox1 and PhGA2ox8 were determined using RT-PCR. Then the bioinformatic analysis of these genes and encoded proteins were performed. The coding sequences of PhGA2ox1 and PhGA2ox8 were amplified by PCR. And then analyzed the bioinformation of these sequences. The expression levels of PhGA2ox1 and PhGA2ox8 were analyzed using qPCR. Results: Bioinformatic analysis showed that PhGA2ox1 contained 981 bp and encoded a predicted protein of 326 amino acids with molecular weight of 36 871.3 and isoelectric point (PI) of 8.66; PhGA2ox8 contained 1 056 bp and encoded a predicted protein of 351 amino acids with molecular weight of 40 169.9 and PI of 6.91. Both PhGA2ox1 and PhGA2ox8 contained GA2ox conserved domains DIOX_N and 20G-Fell_Oxy, without obvious hydrophobic region, transmembrane domain and signal peptide. Phylogenetic analysis showed that the GA2ox of plant could be divided into three classes, PhGA2ox1 bolongs to Class I and PhGA2ox8 belongs to Class III. The qPCR. showed that the expression of PhGA2ox1 in different tissues and organs of P. heterophylla was constant, but the expression of PhGA2ox8 in root xylem was significantly higher than that in other tissues and organs (P < 0.05). Conclusion: The PhGA2ox1 and PhGA2ox8 genes are cloned for the first time, and provide a foundation for they may play an important role in the growth and development process of P. heterophylla.

Plant growth regulators and shade on emergence in macadamia nut tree / Reguladores vegetais e sombreamento na emergência de plântulas de macadâmia

This study aimed to evaluate the effect of plant growth regulators and shading on seed germination of macadamia nut trees. The experiment was conducted in a seedling production nursery of the company "QueenNut Macadâmia" located in the "Palmeiras" Farm, Dois Córregos, São Paulo (SP) state, Brazil. The Treatments were T1: water (control) under a shading screen, T2: GA4+7 + N-(phenylmethyl)-aminopurine (Promalin®) at 200 mL L-1 under a shading screen, T3: Promalin® at 400 mL L-1 under a shading screen, T4: gibberellic acid (GA3) + kinetin (Kt) + 3- indolebutyric acid (IBA) ­ Stimulate® at 5 mL kg-1 seeds under a shading screen, T5: Stimulate® at 10 mL kg-1 of seeds under a shading screen, and T6: water (control) under no shading screen. Seeds were soaked in the solutions or in water containing plant growth regulators for 24 hours. Then, they were dried and sown in sand. 450 seeds were used for each treatment. The evaluations began from seedling emergence by counting the total number of seedlings per treatment. A high average of macadamia nut tree seedling emergence was obtained in the treatments GA4+7 + N-(phenylmethyl)-aminopurine (Promalin®) 400 mL L-1 with Sombrite® (75.7%), GA4+7 + N-(phenylmethyl)-aminopurine (Promalin®) 200 mL L-1 (72.6%) Sombrite® and water (control) without Sombrite® (71.5%).

Este estudo objetivou avaliar os efeitos de reguladores vegetais e sombreamento na emergência de plântulas de macadâmia. O experimento foi conduzido no viveiro de produção de mudas de macadâmia da empresa QueenNut Macadâmia, na Fazenda Palmeiras no município de Dois Córregos-SP. Os tratamentos utilizados foram: T1. água (testemunha) com Sombrite®, T2. GA4+7 + N-(fenilmetil)-aminopurina (Promalin®) a 200 mL L-1 com Sombrite®, T3. Promalin® a 400 mL L-1 com Sombrite®, T4. GA3 + IBA + Kt ­ Stimulate® a 5 mL kg-1 sementes com Sombrite®, T5. Stimulate® a 10 mL kg-1 sementes com Sombrite® e T6. água (testemunha) sem Sombrite®. As sementes foram embebidas em água ou nas soluções contendo os reguladores vegetais durante 24h, secas à sombra e semeadas em sementeiras de areia. Foram utilizadas 450 sementes em cada tratamento. A partir da emergência das plântulas foram iniciadas as avaliações, realizadas através da contagem do número total de plântulas emergidas por tratamento. Os melhores resultados na emergência de plântulas de macadâmia foram obtidos nos tratamentos com GA4+7 + N-(fenilmetil)- aminopurina (Promalin®) a 400 mL L-1 com Sombrite® (75,7%), GA4+7 + N-(fenilmetil)-aminopurina (Promalin®) a 200 mL L-1 (72,6%) com Sombrite® e água (testemunha) sem Sombrite® (71,5%).

Período de formação do cacho e biorreguladores na produção e qualidade da banana 'Grande Naine' / Period of bunch formation and growth regulators on yield and quality of banana 'Grande Naine'

Objetivou-se determinar o efeito do período de formação do cacho e dos biorreguladores na produção e qualidade da banana 'Grande Naine'. O experimento foi realizado em delineamento inteiramente casualizado em parcelas subdivididas, com seis repetições. Consideram-se, nas parcelas, os períodos de formação do cacho, verão e outono, e nas subparcelas, os biorreguladores: duas aplicações com água, giberelina (AG3, 200mg L-1), auxina (2,4 D; 10mg L-1), citocinina (TDZ, 150mg L-1) e a mistura de giberelina, auxina e citocinina (AG3, 56,3mg L-1; AIB, 56,3mg L-1 e ZEA, 101,3mg L-1). Os períodos de formação do cacho influenciam o número de pencas, o tamanho, a vida pós-colheita, o pH, a acidez titulável, a razão sólidos solúveis e acidez titulável e a firmeza do fruto. A mistura de biorreguladores eleva o peso do cacho e da 4ª penca em cachos formados no verão.

The objectives of this study were to determine the effect of climatic conditions during the period of bunch formation and growth regulators on yield and quality of banana 'Grande Naine'. The experiment was conducted in a completely randomized split plot with six replications. It was considered the plots, and the periods of bunch formation, summer and autumn. In the subplots it was considered the growth regulators: two applications were made with water, gibberellin (GA3, 200mg L-1), auxin (2.4-D, 10mg L-1), cytokinin (TDZ, 150mg L-1) and a mixture of gibberellin, auxin and cytokinin (AG3, 56.3mg L-1; AIB, 56.3mg L-1 and ZEA, 101.3mg L-1). Periods of bunch formation influence the number of hands, the size, shelf life, pH, titratable acidity, soluble solids and titratable acidity ratio and the firmness of the fruit. The mixture of growth regulators increase the weight of the bunch and the 4th hand bunches formed in the summer.

Determination of Trace Gibberellin A3 by Magnetic Self-assembly Molecularly Imprinted Electrochemical Sensor / 分析化学

ThemagneticFe3O4nanoparticlesweresynthesizedbyco-precipitationmethod,andthenmagnetic Fe3 O4@Au nanoparticle was synthesized to improve the affinity of particle surface. L-Cys-GA3 was grafted on the surface of gold clad by self-assembly method, and then dropped it on glassy carbon electrode, for further manufacture of MIP/Fe3 O4@Au by using electropolymerzation L-Cys. The surface morphology and particle size distribution of Fe3 O4@Au were studied by TEM. The structure and composition of gibberellins A3, MIP and nMIP were studied by IR. The test system was optimized, and the results showed that when the cycles of electropolymerization was 30, acetic acid:methanol (1:8, V/V) was chosen as eluent, elution time was optimized for 5 min and rebinding time for 7 min, the sensor got a high stability and good recognition ability for gibberellins A3 . The concentration of gibberellins A3 in the range of 1 . 0 × 10-11-1 . 0 × 10-8 mol/L had a relationship with the oxidation peak current of probe, with the detection limit of 2. 57×10-12 mol/L. The sensor was successfully used for the determination of GA3 in beer sample.

Germinação de sementes e vigor de plântulas de maracujazeiro amarelo submetidos à ação do ácido giberélico / Seed germination and seedling vigor of passion fruit submitted to the action of gibberellic acid

Objetivou-se avaliar a ação do ácido giberélico (GA3) na germinação de sementes e vigor de plântulas de maracujazeiro amarelo (Passiflora edulis Sims f. flavicarpa Deg ). Sementes de maracujazeiro amarelo foram pré-embebidas em soluções de ácido giberélico a 4%, por seis horas, nas seguintes concentrações: 0,5; 1,0; 2,0; 4,0 mL por litro de solução, e água destilada como controle. As sementes foram distribuídas em papel de germinação e mantidas em germinador, à temperatura de 25ºC com fotoperíodo de 12 horas. O teste de vigor foi conduzido simultaneamente com o teste padrão de germinação e as plântulas avaliadas aos 14 dias após a semeadura (DAS). O delineamento experimental foi inteiramente casualizado com cinco tratamentos e quatro repetições, com 50 sementes em cada rolo de papel. Avaliou-se a porcentagem de germinação de sementes, plântulas normais na primeira contagem, plântulas anormais, sementes mortas, sementes firmes, comprimento de raiz, da parte aérea e total de plântulas e índice de velocidade de emergência (IVE). Os dados foram submetidos à análise de variância e para as médias dos tratamentos foram ajustadas equações de regressão polinomial. Os resultados indicam que a pré-embebição das sementes de maracujazeiro amarelo com GA3, estimula a porcentagem de germinação e reduz a porcentagem de sementes mortas. O regulador vegetal proporciona incremento no comprimento da parte aérea das plântulas de maracujazeiro amarelo.

The objective was to evaluate the effect of gibberellic acid (GA3) on seed germination and seedling vigor of passion fruit (Passiflora edulis Sims f. flavicarpa Deg). Seeds of passion fruit were pre-soaked in solutions of gibberellic acid to 4%, for six hours in the following concentrations: 0,5; 1,0; 2,0; 4,0 mL per liter of solution and distilled water as control. The seeds were spread on germination paper and kept in a germination chamber at a temperature of 25 °C with a photoperiod of 12 hours. The current test was conducted simultaneously with the pattern of germination and seedlings measured at 14 days after sowing (DAS). A completely randomized design with five treatments and four replicates with 50 seeds in each roll. It was evaluated the percentage of seed germination, seedling normal on the first count, abnormal seedlings, dead seeds, seed firm, root length, shoot and total seedling index and emergence rate (IVE). The data was subjected to analysis of variance and the average treatment were adjusted polynomial regression equations. The results indicate that pre-soaking the seeds of passion fruit with GA3, stimulates germination and reduces the percentage of dead seeds. The growth regulator provides an increase in the length of the tops of yellow passion fruit.

Produção, florescimento e frutificação de tangerineira 'Poncã' submetida à aplicação de ácido giberélico / Production, florescence and fruitfication of Pokan mandarin tree submitted to gibberellic acid application

O florescimento e a produção de tangerinas são influenciados pela safra anterior. Assim, a inibição do florescimento excessivo pode evitar uma frutificação elevada e a exaustão das reservas da planta, contribuindo para uma produção mais uniforme ao longo dos anos. O objetivo deste trabalho foi avaliar o efeito da aplicação de giberelina na redução do florescimento em tangerinas 'Poncã' e na produção de frutos em duas safras consecutivas. Os resultados demonstram que a aplicação de ácido giberélico reduziu a emissão de flores e aumentou a porcentagem de pegamento de frutos em relação ao ano de florescimento excessivo. A aplicação de giberelinas cerca de 90-150 dias antes do pleno florescimento pode contribuir para redução do efeito da alternância de produção.

The florescence and the production of tangerines are influenced by the last production. So, the inhibition of an excessive florescence can avoid a great production and the exhaustion of the plant reserves, contributing to a more uniform production every year. The objective of this work was to evaluate the effect of the reduction of the florescence in Ponkan mandarin trees upon the production of fruits in two consecutive productions. The results demonstrated that the gibberellic acid application reduced the flower production and increased the percentage of fruit production in relation to the year with an excessive flowering. The application of the gibberellic acid around 90-150 days before the full flowering period can contribute to the reduction of the effect of the production alternance.

Influence of jasmonic acid on endogenous gibberellin and abscisic acid in salt-stressed chard plant.

The endogenous gibberellin (GA) and abscisic acid (ABA) contents as an effect of different application times of jasmonic acid (JA) in chard seedlings exposed to salt stress were investigated. Endogenous ABA content was increased by JA treatment after NaCl treatment, rather than after JA application before NaCl treatment. JA application after NaCl treatment caused higher ABA content than treatment with 160 mM NaCl alone. Total gibberellin content decreased after NaCl stress, but NaCl-reduction in total GA contents counteracted by exogenous JA. Total endogenous GA contents were increased in JA treatment after NaCl and were highest at 24 hr of JA application before NaCl exposure. JA treatment promoted the increase of dry weight compared to chard plant exposed to 160 mM NaCl. Thus, JA presumably induces gibberellin biosynthesis showing the promotion of growth and dry weight of chard plants under salt stress.

Effects of exogenous hormones on growth and tanshinones accumulation in radix of Salvia miltiorrhiza / 中草药

Objective To study the effects of exogenous hormones,gibberellin(GA3)and indole-3-acetic acid(IAA),on growth and tanshinones accumulation in the radix of Salvia miltiorrhiza.MethodsThe herb characters and contents of tanshinones(cryptotanshinone,tanshinone Ⅰ,and tanshinone ⅡA)in the radix of S.miltiorrhiza with different hormone treatments were investigated and compared in the combination of trail pot and indoor analysis.Results It promoted aboveground biomass in the radix of S.miltiorrhiza increasing with single GA3.But it inhibited underground biomass increasing.The exogenous addition of low-level and high-level GA3 was benefit for tanshinones accumulation,whereas the middle-level GA3 wasn't.The aboveground and underground biomass in the radix of S.miltiorrhiza increased indistinctively with the increasing of IAA concentration and then decreased.And the IAA solutions also increased plant height and root length indistinctively.in a whole,it was benefit for tanshinones accumulation when applying low-level IAA(0.5 mg/L)singly.With the increasing of IAA concentration,the content of cryptotanshinone increased,but the contents of tanshinone Ⅰ and tanshinone ⅡA decreased.It promoted S.miltiorrhiza radix growth and three tanshinones contents increasing obviously with the combined application of two low-level hormones.Conclusion The applying of GA3 and IAA is benefit for the growth and three tanshinones accumulation in the radix of S.miltiorrhiza.