ABSTRACT
Objective To study the chemical constituents of Glechoma longituba. Methods Chemical constituents were extracted by organic solvents, isolated and purified by chromatographic techniques with silica gel, Sephadex LH-20, semi-preparative HPLC, and recrystallization. Their structures were elucidated on the basis of physiochemical and spectral analyses. Results Thirteen compounds were obtained and elucidated as ursolic acid (1), asoleanolic acid (2), protocatechualdehyde (3), betulic acid (4), luteolin (5), β-sitosterol (6), lutin (7), syringic acid (8), 4-acetoxy-3,5-dimethoxybenzoicacid (9), 2,5-dihydroxy-1,4-benzenedicarboxylic acid (10), (E)-3-[4-(carboxymethoxy)-3-methoxyphenyl] acrylic acid (11), emodin (12), and loliolide (13). Conclusion Compounds 8-10 and 12 are isolated from this plant for the first time. Compounds 11 and 13 are isolated from the plants of Glechoma Linn. for the first time.
ABSTRACT
Objective: To study the chemical constituents from Glechoma longituba and their anti-oxidant activity in vitro. Methods: The chemical constituents were isolated and purified by solvent extraction, repeated silica gel column chromatography, and reverse phase preparative HPLC. Their structures were identified on the basis of ESI-MS and NMR spectra. The anti-oxidant activities of these compounds were screened by DPPH free radical scavenging assay in vitro. Results: Ten compounds were isolated and elucidated as cosmosiin (1), dihydrocaffeic acid (2), caffeic acid (3), isololiolide (4), vomifoliol (5), glecholone (6), apigenin (7), aurantiamide (8), (+)-lariciresinol (9), and (-)-syringaresinol (10). Conclusion: Compounds 2, 4, and 8-10 are isolated from the plants in Glechoma L. for the first time; Compounds 9 and 10 are lignans firstly obtained from this plant. DPPH free radical scavenging assays show that compounds 2, 3, 9, and 10 exhibit potential anti-oxidant activity.