ABSTRACT
Resumen Se evaluó el efecto de la suplementación con ensilaje de estiércol porcino sobre indicadores productivos de novillas raza Hartón del Valle, Se utilizó un diseño Irrestrictamente al azar, se aplicó pruebas de normalidad distributiva, análisis de varianza y prueba de Tukey, los animales fueron asignados al azar a dos grupos experimentales. Los dos grupos consumieron como base forrajera, pasto King grass morado (Pennisetum purpureum CV King grass) y los ensilajes suministrados a las novillas contenían dos niveles de inclusión del estiércol 15% (T1) y 30% (T2). Se seleccionaron 12 hembras destetadas de la línea Zanjón Hondo, con peso promedio de 218 kg ± 6 kg, de 12 meses de edad, donde se determinaron por un periodo de 45 días, los indicadores productivos de consumo, cambio de peso y conversión alimenticia. Los dos niveles de suplementación con porquinaza no afectaron (P>0.05) el consumo voluntario de materia seca (CVMS) 6,58 (T1) y 7,06 (T2) kg/MS/animal/día. Sin embargo, si hubo diferencias (P< 0.05) en ganancia diaria de peso, las cuales fueron de 0,53 para T1 y 0,38 kg/día en T2. La conversión alimenticia se estimó en 11,66 en T1 y 18,89 en T2 (P<0.05). Se concluye que los indicadores productivos de novillas Hartón del Valle resultan satisfactorios al incorporar 15 % de estiércol de cerdo deshidratado en el ensilaje como el alimento suplementario en el periodo de levante.
Abstract The effect of porcine manure silage supplementation on productive indicators of Hartón del Valle heifers was evaluated. An Irresistibly Random design was used, distributive normality tests, analysis of variance and Tukey test were applied. The animals were randomly assigned to two experimental groups. Both groups consumed forage purple king grass (Pennisetum purpureum CV King grass) and silage supplied to heifers contained two levels of inclusion of manure 15% (T1) and 30% (T2). Twelve weaned females were selected from the Zanjón Hondo line, with a mean weight of 218 kg ± 6 kg, of 12 months of age, where were determined for a period of 45 days, productive indicators of consumption, weight change and feed conversion. The two levels of supplementation with porkase did not affect (P>0.05) voluntary dry matter intake (CVMS) 6.58 (T1) and 7.06 (T2) kg/d.m./animal/día. However, there were differences (P<0.05) in daily weight gain, which were 0.53 for T1 and 0.38 kg / day for T2. Feed conversion was estimated at 11.66 for T1 and 18.89 for T2 (P<0.05). It is concluded that the productive indicators of Hartón del Valle heifers are satisfactory by incorporating 15% of dehydrated pig manure in silage as the supplementary feed in the period of rising.
Resumo Avaliou-se o efeito da suplementação com silagem de suínos em indicadores produtivos de novilhas Hartón del Valle, utilizando-se um desenho Irresistivelmente Random, testes de normalidade distributiva, análise de variância e teste de Tukey, os animais foram divididos aleatoriamente em dois grupos experimentais. Ambos grupos consumiram como base de forragem a grama king grass roxa (Pennisetum purpureum CV King grass) e os silagem para as novilhas tinham dois níveis de inclusão de estrume de 15% (T1) e 30% (T2). Foram selecionados 12 fêmeas recém-desmamados da linha Zanjón Hondo, com um peso médio de 218 kg ± 6 kg, 12 meses de idade, onde foram determinadas por um período de 45 dias os indicadores de consumo produtivas, mudança de peso e conversão alimentar. Os dois níveis de suplementação porquinasa não afetou (P>0.05) a ingestão voluntária de matéria seca (CVMS) 6,58 (T1) e 7,06 (T2) kg/MS/animal/día. No entanto, se houve diferenças (P<0.05) no ganho de peso diário, que foram 0,53 para T1 e 0,38 kg/dia para T2. A conveção alimentar estimo-se em 11,66 em T1 e 18,89 em T2 (P<0.05). Conclui-se que os indicadores de novilhas produtivas Harton del Valle são satisfatórios para incorporar 15% da silagem de estrume de porco deidratado como o alimento-suplemento no período de criação.
ABSTRACT
Objective: To evaluate the cytotoxic activities of Premna odorata (P. odorata) leaves and bark, Artocarpus camansi (A. camansi) and Gliricidia sepium against selected human cancer cell lines by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methods: The crude extracts of P. odorata, A. camansi and Gliricidia sepium were subjected to liquid-liquid partitioning by using hexane and ethyl acetate to separate compounds based on their polarity. The fractions were tested for their cytotoxic activity against human colon cancer cell line (HCT116), breast cancer cell line (MCF-7), lung adenocarcinoma cell line (A549) and Chinese hamster ovary cell line (AA8) by using MTT assay. Results: Based on the standard values of toxicity set by the study of Suffness and Pezzuto, P. odorata leaves and P. odorata bark hexane fractions and A. camansi leaves were all considered highly cytotoxic against the selected human cancer cell lines. P. odorata bark hexane extract exhibited the highest selectivity index for HCT116, MCF-7 and A549 cancer cell lines. Conclusions: The results obtained indicated that P. odorata leaves and bark and A. camansi leaves have excellent cytotoxic activity and warrant further studies to isolate novel compounds for chemotherapeutic use.
ABSTRACT
Objetivou-se, nesse trabalho, obter informações sobre a diversidade de abelhas visitantes em gliricídia (Gliricidia sepium (Jacq.) Stend.), na região do Recôncavo Baiano. As espécies visitantes nas flores foram coletadas, no período de setembro a novembro de 2005. Definiu-se, aleatoriamente, 15 inflorescências por intervalo de hora para a coleta efetiva das abelhas, sendo utilizado um minuto por inflorescência, ao longo do intervalo de 07:00 às 18:00 horas, durante o período de floração. Um total de 10 espécies foram identificadas, sendo que Apis mellifera foi a espécie mais abundante, com freqüência relativa igual a 25,40 %, seguida da Trigona spinipes (23,81 %), Nannotrigona testaceicornis (14,28 %), Xylocopa grisescens e Trigona fuscipennis (ambas com 12,70 %). O pico de visita das abelhas ocorreu das 10:01 às 11:00 horas. A. mellifera, T. spinipes e N. testaceicornis foram consideradas as espécies com potencial para a polinização das flores de gliricídias, na região do Recôncavo Baiano.
The focus of this paper was to get information about the diversity of bees visiting Gliricidia sepium (Jacq.) Stend. in the Recôncavo Baiano region. The species visiting the flowers were collected in the period from September to November 2005. For the collection of the bees, 15 inflorescences were defined randomly per hour, one minute being used per inflorescence, throughout the interval from 07:00 a.m. to 06:00 p.m., during the blooming period. A total of ten species were identified, Apis mellifera being the most abundant species, with relative frequency equal to 25.40%, followed by Trigona spinipes (23.81%), Nannotrigona testaceicornis(14.28%), Xylocopa grisescens, and Trigona fuscipennis (both with 12.70%). The peak of bee visits occurred from 10:01a.m. to 11:00 a.m. A. mellifera, T. spinipes, and N. testaceicornis were considered the species with potential for the pollination of Gliciridia sepium flowers in the region of the Recôncavo Baiano.
ABSTRACT
Bemisia tabaci is an important virus vector on a number of crops worldwide. Therefore, a preventive approach to deal with viral epidemics may be the deployment of repellents or phagodeterrents at earlier stages of plant development (critical period). Thus, the crude extract and four fractions thereof (water, water:methanol, methanol, and diethyl ether) of mother-of-cocoa (Gliricidia sepium, Fabaceae) were tested for phagodeterrence to B. tabaci adults under greenhouse conditions, on tomato plants, in Costa Rica. Both restricted-choice and unrestricted-choice experiments showed that the crude extract and some fractions exerted such effect on the insect. In the former (in sleeve cages), three fractions caused deterrence at doses as low as 0.1% (methanol), 0.5% (water:methanol) and 1.5% (diethyl ether). However, in the latter (plants exposed in a greenhouse) no one of the fractions performed well, suggesting that the deterrent principles somehow decomposed under the experimental conditions. Rev. Biol. Trop. 56 (4): 2099-2113. Epub 2008 December 12.
Mundialmente, Bemisia tabaci es un importante vector de virus en numerosos cultivos. Por tanto, un enfoque preventivo para enfrentar las epidemias virales podría ser el empleo de sustancias repelentes o fagodisuasivas en las etapas tempranas del desarrollo de las plantas (período crítico). Así, tanto el extracto crudo como cuatro fracciones (agua, agua:metanol, metanol y éter dietílico) del madero negro (Gliricidia sepium, Fabaceae) fueron evaluadas en cuanto a su actividad fagodisuasiva sobre los adultos de B. tabaci en condiciones de invernadero, utilizando plantas de tomate, en Turrialba, Costa Rica. Tanto los experimentos de escogencia restringida como los de escogencia irrestricta revelaron que el extracto crudo y algunas fracciones mostraron dicha actividad. En los primeros experimentos (en jaulas de manga), tres fracciones causaron fagodisuasión a dosis tan bajas como 0.1% (metanol), 0.5% (agua:metanol) y 1.5% (éter dietílico). Sin embargo, en los segundos (plantas expuestas dentro de un invernadero) ninguna de las fracciones lo hizo, lo cual sugiere que los principios fagodisuasivos perdieron su actividad en esas condiciones experimentales.
Subject(s)
Animals , Fabaceae/chemistry , Hemiptera/drug effects , Plant Extracts/pharmacology , Feeding Behavior/drug effects , Hemiptera/physiologyABSTRACT
Long term applications of leguminous green mulch could increase mineralizable nitrogen (N) beneath cupuaçu trees produced on the infertile acidic Ultisols and Oxisols of the Amazon Basin. However, low quality standing cupuaçu litter could interfere with green mulch N release and soil N mineralization. This study compared mineral N, total N, and microbial biomass N beneath cupuaçu trees grown in two different agroforestry systems, north of Manaus, Brazil, following seven years of different green mulch application rates. To test for net interactions between green mulch and cupuaçu litter, dried gliricidia and inga leaves were mixed with senescent cupuaçu leaves, surface applied to an Oxisol soil, and incubated in a greenhouse for 162 days. Leaf decomposition, N release and soil N mineralization were periodically measured in the mixed species litter treatments and compared to single species applications. The effect of legume biomass and cupuaçu litter on soil mineral N was additive implying that recommendations for green mulch applications to cupuaçu trees can be based on N dynamics of individual green mulch species. Results demonstrated that residue quality, not quantity, was the dominant factor affecting the rate of N release from leaves and soil N mineralization in a controlled environment. In the field, complex N cycling and other factors, including soil fauna, roots, and microclimatic effects, had a stronger influence on available soil N than residue quality.
Aplicações a longo prazo de leguminosas como adubo verde podem aumentar o nitrogênio (N) mineralizável sob árvores de cupuaçu em solos pouco férteis e ácidos (Ultisols e Oxisols) da Bacia Amazônica. Entretanto, a baixa qualidade da liteira de cupuaçu pode influênciara liberação de N do adubo verde e a mineralização deste no solo. Neste estudo foram comparados o N mineral, N total, e o N da biomassa microbiana sob árvores de cupuaçu cultivadas em dois sistemas agroflorestais, ao norte de Manaus, Brasil, as quais receberam diferentes aplicações de adubo verde sob sua liteira natural durante sete anos. Para testar as interações entre o adubo verde e a liteira de cupuaçu, folhas secas de gliricídia e ingá foram misturadas com as folhas senescentes de cupuaçu, distribuídas na superfície de um solo Oxisol, e incubadas em casa de vegetação durante 162 dias. A decomposição das folhas, a liberação de N e mineralização do N no solo foram periodicamente mensurados nos tratamentos de mistura de liteira de diferentes espécies e comparados com as aplicações de liteira de apenas uma espécie. O efeito da biomassa de leguminosas e da liteira de cupuaçu no N mineral do solo foi aditivo, indicando que o uso de adubação verde em plantas de cupuaçu pode ser baseado na dinâmica do N em cada espécie usada como adubo verde. Os resultados demonstraram que a qualidade do resíduo, e não a quantidade, foi o principal fator que influenciou a taxa de liberação de N das folhas e a mineralização deste no solo, em ambiente controlado. No campo, o complexo ciclo do N e outros fatores tais como a fauna do solo, raízes e os efeitos do microclima, tiveram uma influência mais forte na disponibilidade de N no solo do que a qualidade d o resíduo vegetal.
Subject(s)
Soil , Forestry , Fruit , FabaceaeABSTRACT
Para conocer el efecto de suplementación con harina de tres arbustivas forrajeras tropicales, en el comportamiento productivo y fermentación ruminal de corderos en crecimiento, se realizó un estudio durante 90 días, utilizando 30 corderos con encaste de Pelibuey y Black Belly (18,29 ± 1,56 kg PV), mantenidos en pastoreo continuo en estrella africana (Cynodon plestostachyus; 75,5% FDN y 6,1% PC) de 7:00 a.m. a 4:00 p.m, posteriormente los animales fueron alojados en corraletas individuales, proporcionándoles 200 g animal-1 d-1 de suplemento de acuerdo a los tratamientos (T) evaluados: T1 = sólo pastoreo (SP), T2 = T1+concentrado comercial (CC), T3 = T1+harina de cocoíte (Gliricidia sepium; HC), T4 = T1+harina de morera (Morus alba; HM) y T5 = T1+harina de tulipán (Hibiscus rosa-sinensis; HT). Las variables registradas fueron: consumo de suplemento y pasto, ganancia diaria de peso (GDP), digestibilidad aparente de materia seca del suplemento y del pasto. En fluido ruminal se midió pH, concentración de ácidos grasos volátiles, nitrógeno amoniacal, bacterias totales y protozoarios. El CC y la HT presentaron el mejor (P < 0,05) consumo de suplemento (CC, 181,6ª; HC, 97,7c; HM, 149,7b; HT, 167,2ab g d-1), de materia seca total (SP, 789,1b; CC, 976,8ª; HT, 941,8ª g d-1) y la mejor GDP (SP, 46,1b; CC, 81,6ª; HT, 77,1ª; g d-1). El CC propició la más alta (P < 0,05) digestibilidad aparente de la materia seca (DAMS, 60,97ª); en contraste la más baja (P < 0,05) DAMS se presentó con el uso de HC (SP, 49,27ab; CC, 54,00ª; HC, 48,52C; HM, 50,47ab; HT, 50,54ab). En las variables ruminales, no se observaron diferencias entre tratamientos para pH, nitrógeno amoniacal y AGV; solamente se registraron cambios (P < 0,05) en la concentración (1 × 10(8) mL-1) de bacterias totales (SP, 45,0ª; HM, 1,1b) y de protozoarios (SP, 20,73b; CC, 96,01ª; 1 × 10(4) mL-1). La harina de tulipán mostró una respuesta similar a la del CC. La menor respuesta se obtuvo con HC. Por lo anterior se recomienda HT como sustituto de CC para corderos en pastoreo en el trópico.
A study was carried out in order to determine the effect of a commercial feed and three different meals produced by tropical forage shrubs, on productive performance, digestibility and some rumen variables, when used as supplements for grazing lambs. Thirty crossbreed (Pelibuey X Black Belly) male lambs (18.29 ± 1.56 Kg BW), were used in a 90 d study. Animals were on continuous grazing of African star (Cynodon plestostachyus; (75.5% NDF, 6.1% CP) from 7:00 am to 4:00 pm. After grazing animals were housed in individual pens and fed (200 g animal-1 d-1)any of four supplements, according to treatment: T1 = grazing (G); T2 = T1 + commercial concentrate (CC); T3 = T1 + cocoite meal (Gliricidia sepium; CM); T4 = T1 + morera meal (Morus alba; MM), and T5 = T1 + tulipan meal (Hibiscus rosa-sinensis; TM). Supplement and forage intake, dry matter digestibility and daily weight gain, were measured. Volatile fatty acids (VFA), ammonia nitrogen concentration, total bacteria and protozoa were determined in rumen liquor. Differences in supplement intake (P < 0.05) (CC, 181.6ª; CM, 97.7c; MM, 149.7b; TM, 167.2ab g d-1), dry matter intake (OG, 789.1b; CC, 976.8ª; TM, 941.8ª g d-1) and daily weight gain (OG, 46.1b; CC, 81.6ª; TM, 77.1ª; g d-1) were found. The highest (P < 0.05) percentage of dry matter digestibility (DMD) was found for CC (CC, 60.97ª). Forage DMD was the lowest for CM (OG, 49.27ab; CC, 54.00ª; CM, 48.52C; MM, 50.47ab; TM, 50.54ab). No differences (P > 0.05) were found for rumen pH, ammonia nitrogen and VFA concentration among treatments. Compared with OG treatment, supplementation with MM decreased (P < 0.05) total bacteria concentration (OG, 45.0ª vs MM, 1.1b × 10(8) mL-1)whilst CC increased protozoa concentration (OG, 20.73b vs CC, 96.01ª × 10(4) mL-1). Supplementation with TM showed similar results that those observed with CC. The lowest response was observed for CM and OG. It is concluded that TM can be used as a supplement instead of CC in the tropic for grazing lambs.