Significance of combined determination of three autoantibodies in predictive of type 1 diabetes mellitus / 中华检验医学杂志

Objective To evaluate the significance of combined determination of glutamic acid decarboxylase antibodies(GADA),islet cell antibodies(ICA) and insulin autoantibodies (IAA) in predictive of type 1 diabetes mellitus Methods GADA, ICA and IAA were determined by enzyme linked immunosorbent assay and C peptide by radioimmunoassay Diagnose efficiency were calculated by matrix decision method Results The positive rate of GADA,ICA and IAA(68 8%,43 0% and 39 8%) were significantly elevated in the patients with type 1 diabetes mellitus compared with type 2 diabetes mellitus or normal subjects ( P