Descompresión y posterior enucleación de un ameloblastoma uniquístico-variante de células granulares. Reporte de caso / Decompression and posterior enucleation of a unicystic ameloblastoma- granular cell variant. Case report

El ameloblastoma es uno de los tumores benignos más agresivos y destructivos localmente de la región maxilofacial, capaz de generar grandes deformaciones faciales en un corto periodo. Sin embargo, dentro de su clasificación, la variante uniquística es considerada la menos agresiva y permite un manejo conservador de esta patología. Este artículo describe un caso clínico de una mujer de veinte años de edad, a quien se le diagnosticó un ameloblastoma uniquístico de células granulares, variante histológica poco frecuente. La lesión fue manejada de manera conservadora, realizando una descompresión durante dos meses y una enucleación con resección de los márgenes óseos. Este tratamiento disminuye la morbilidad asociada a la resección quirúrgica y se evitan grandes defectos estéticos y funcionales.

The ameloblastoma is one of the most agressive and localy destructive benign tumors of the human body, capable of producing enormous deformations of the face in a short period of time. In its classification the unicystic variant is consider less agresive and allows a conservative treatment. We present a case of 20 years old female patient, with a unicystic ameloblastoma with a granular cells variant, which was mana - ged in a conservative way, with a two month decompression and a posterior enucleation, with a one year follow up. This treatment is less morbid than the surgical resection with safety margins, avoiding big stetical and functional defects

Characterization of miRNA-483-5p and targeted gene ERK1 in the regulation of proliferation-apoptosis balance of human granulosa cells / 安徽医科大学学报

Objective To demonstrate that miR-483-5p and its targeted gene ERK1 involved in the regulation of proliferation and apoptosis of human granulosa cells.Methods ① The ERK1 mRNA and protein level expression were detected by PCR and Western blot after miR-483-5p overexpression in vitro normal human granular cells.De-tected the relative luciferase density after cotransfecting miR-483-5p mimics and its control respectively with wild or mutant ERK1 mRNA 3`UTR cloned luciferase report vector in granular cells.② Granular cells proliferation and ap-optosis were detected by MTT and TUNEL after transfecting miR-483-5p mimics or ERK1 siRNAs alone or simulta-neously.Results ① It showed that both ERK1 mRNA and protein in granular cells were markedly downregulated after the transfection of miR-483-5p mimics.A significant relative luciferase activity decrease were detected in the granular cells co-transfected with ERK1 wild and miR-483-5p mimics comparison with the control mimics,but not in the cells co-transfected with the ERK1 mutant and miR-483-5p mimics.② When miR-483-5p mimics and ERK1 siRNAs were alone or co-transfected into granular cells,the proliferation was inhibited while apoptosis trend was monitored to be promoted in all cases.No obvious statistic difference was shown between each other.Conclusion MiR-483-5p is involved in the regulation of the proliferation and apoptosis of human granulosa cells by directly binding to the target gene ERK1.

Tumor de células granulares de esófago / Tumor of granular cells of esophagus

Los tumores de células granulares son lesiones raras y asintomáticas, generalmente un hallazgo incidental en la endoscopia alta o baja. Fueron descritos por primera vez en 1926 por Abrikossoff. Las localizaciones más frecuentes son la mucosa bucal, la dermis y el tejido celular subcutáneo, la mayor parte de estos tumores tienen una naturaleza benigna. Presentamos el caso clínico de una mujer, de 44 años con historia de pirosis desde hace aproximadamente 1 año, se observa por endoscopia superior lesión elevada de 8 mm en tercio distal de esófago y se confirma por estudio histológico el diagnóstico de tumor de celulas granulares. El tratamiento de elección de esta lesión es la polipectomía endoscópica. A pesar de que el potencial maligno es escaso se sugiere seguimiento estrecho clínico y endoscópico

Granular cells tumors are rare and asymptomatic lesions and by general, it is an incidental finding en high or low endoscopy. They were described for the first time by Abrikossoff in 1926. The more frequent locations are the buccal mucosa, dermis and subcutaneous cellular tissue, most of these tumors has a benign origin. This is the case of a woman aged 44 with a pyrosis history from a year ago; by high endoscopy it is noted a 8mm lesion distal to esophagus and confirmed by histological study of granular cells tumor. Elective treatment of this lesion is the endoscopic polypectomy. Despite that the malign potential is low; we suggested a close clinical and endoscopic follow-up

Tumor de células granulares da laringe na infância: relato de caso / Granular cell tumor of the larynx in children: a case report

O tumor de células granulares (TCG) é uma neoplasia incomum, de evolução lenta, na maioria dos casos de caráter benigno e que pode acometer qualquer órgão do corpo. Entre as hipóteses que tentam explicar sua origem, a teoria da gênese neural apresenta embasamento sólido e é a mais aceita atualmente. O TCG é mais comum na raça negra, entre a 4ª e 5ª décadas de vida, acometendo com maior freqüência a região da cabeça e pescoço. A localização laríngea é rara, e quando ocorre é mais comum na porção posterior. É muito raro em crianças em geral acomete a porção anterior da subglote, podendo estender-se para a glote. O sintoma predominante é a rouquidão, podendo ocorrer disfagia, dor, tosse, hemoptise, e estridor. Macroscopicamente o TCG se manifesta como nódulo de pequeno tamanho, firme, séssil ou pediculado, não-ulcerado, de coloração clara, e usualmente bem circunscrito, porém sem cápsula. À microscopia, as granulações citoplasmáticas são características, apresentando positividade para a imunoperoxidase S100 e para a enolase neurônio-específica. O tratamento do TCG laríngeo consiste na exérese cirúrgica. Neste trabalho descrevemos um caso pediátrico de TCG laríngeo e sua evolução clínica após a remoção cirúrgica, alertando para o diagnóstico do TCG na população pediátrica. Foi realizada revisão de literatura abrangendo as características clínicas e histopatológicas do TCG, assim como as formas atuais de tratamento.

The granular cell tumor (GCT) is an uncommon neoplasm, with slow progression, usually benign, that can be found in any organ. The most common region for GCT involvement is in the head and neck. Laryngeal involvement is uncommon and accounts for 6 to 10 percent of all cases reported. Among the major theories of origin and based on the strongest evidence, the most accepted one is that the tumor stems from neuronal tissue. The GCT has a higher incidence in African-descendent patients, and most commonly in their 4th and 6th decades of life. The posterior larynx is the most common laryngeal site. Pediatric laryngeal GCT is rare, anterior subglottis involvement has been described and extensive glottic involvement may occur. Affected patients typically present with hoarseness, dysphagia, cough, haemoptysis, stridor and pain. The GCT presents as a small, firm nodule, sessile or polypoid, with intact mucosa, well outlined but not encapsulated. Cytoplasm granules are typically seen under light microscopy, and the cells are positive for S100 immunoperoxidase and neuron-specific enolase. Treatment of laryngeal GCT is based on surgical excision. This paper describes a pediatric patient with GCT and its clinical course before and after surgical treatment, stressing the importance of GCT diagnosis in the pediatric population. We review clinical course, pathology characteristics and treatment.

Congenital Epulis: A Report of Two Cases

We present two cases of congenital epulis in female newborns. Congenital epulis is a very rare lesion of uncertain histogenesis. The present lesions were located on the gingiva of the anterior alveolar ridge of the maxilla and the mandible, respectively. Both tumors consisted mainly of large eosinophilic granular cells arranged in solid nests. The neoplastic granular cells showed positive reactions for neuron specific enolase and vimentin in their cytoplasms, while they were entirely negative for other antibodies used in this study.