ABSTRACT
RESUMEN Los tumores productores de esteroides ováricos constituyen entidades infrecuentes y son potencialmente malignos. La testosterona es la hormona que se eleva con más frecuencia y se asocia en su mayoría a signos de virilización. Se diagnosticó un caso de una paciente de 62 años con tumor gigante virilizante de células de la granulosa de ovario. Se realizo laparotomía programada con biopsia por congelación e histerectomía total abdominal con doble anixectomía más omentectomía. Evoluciono favorablemente sin complicaciones.El caso que aquí se reporta es el de una paciente con antecedentes de salud aparente, menstruaciones regulares, con antecedentes obstétricos de 4 gestaciones y de haber sido operada a los 24 años de un óbito fetal de mellizos, ante un cuadro de síndrome de virilización se utilizaron los datos provenientes de la historia clínica hospitalaria, exámenes complementarios, la entrevista y el examen físico de la paciente. A pesar del tiempo de evolución que presentaba la paciente con el tumor virilizante gigante de células de la granulosa de ovario izquierdo, la operación fue un éxito y la evolución ha sido satisfactoria, gracias a la labor del personal médico que estuvo a cargo del caso, demostrando una vez más las fortalezas del sistema cubano de salud ante situaciones como estas.
ABSTRACT Peripheral steroid-producing tumors are rare entities and are potentially malignant. Testosterone is the most commonly elevated hormone and is mostly associated with signs of virilization. A case of a 62-year-old patient with a giant virilizing ovarian granulose cell tumor was diagnosed. Scheduled laparotomy was performed with freezing biopsy and total abdominal hysterectomy with double anixectomy plus omentectomy. I evolve favorably without complications. The case reported here is that of a patient with a history of apparent health, regular menstruations, with an obstetric history of 4 gestations and having been operated on at 24 years of a fetal heart rate, before a table of virilization syndrome were used data from hospital medical history, complementary examinations, interview and physical examination of the patient. Despite the time of evolution presented by the patient with the giant virilizing tumor of cells of the left ovary granulose, the operation was a success and the evolution has been satisfactory, thanks to the work of the medical staff who were in charge of the case, demonstrating once again the strengths of the Cuban health system in situations like these.
RESUMO Tumores periféricos produtores de esteroides são entidades raras e são potencialmente malignos. A testosterona é o hormônio mais comumente elevado e está principalmente associado com sinais de virilização. Um caso de um paciente de 62 anos com um tumor gigante de células de granulose ovariana foi diagnosticado. A laparotomia programada foi realizada com biópsia congelante e histerectomia abdominal total com anixectomia dupla mais omentectomia. Eu evoluo favoravelmente sem complicações. O caso aqui relatado é o de um paciente com histórico de saúde aparente, menstruações regulares, com histórico obstétrico de 4 gestações e tendo sido operado aos 24 anos de frequência cardíaca fetal, antes de uma tabela de síndrome de virilização ser utilizado dados do histórico médico hospitalar, exames complementares, entrevista e exame físico do paciente. Apesar do tempo de evolução apresentado pelo paciente com o tumor virilizador gigante de células do ovário esquerdo granulose, a operação foi um sucesso e a evolução tem sido satisfatória, graças ao trabalho da equipe médica responsável pelo caso, demonstrando mais uma vez os pontos fortes do sistema de saúde cubano em situações como essas.
ABSTRACT
Objective To investigate the role of mitogen-activated protein kinase(MAPK)signaling pathway regulating synthesis function of steroid hormone of porcine ovarian granulose cells.Methods Porcine ovarian granulose cells were cultured in medium added with dimethyl sulfoxide(DMSO)at concentration of 50μmol/L or with MAPK inhibitor PD98059 (PD) for 48 hours.Then,granulose cells in DMSO medium were added with activator of adenylate cyclase(20 μmoL/L)or blank agent for next 48 hours incubation,which were defined as observation group 1 and control group 1,similarly,granulose cells in PD medium were also added with activator of adenylate cyclase(20 μmol/L)or blank agent for next 48 hours,which were defined as observation group 2 and control group 2.The level of estradiol(E_2),progesterone (P)and testosterone (T) were detected by chemoluminescence.The mRNA expression of 17alpha-hydroxylase/17,20-lyase (CYP17) and aromatase P450 (P450 arom) were assessed by RT-PCR.Results (1)Hormone expression:the level of T,P and E_2 were(29.5±2.5)nmol/L,(80±5)nmol/L,(49±4) pmol/L in control group 1 and(42.3±3.4)nmol/L,(170±15)nmol/L,(75±6)pmol/L in control group 2,which showed significant difference(P<0.05).In the mean time,the level of T, P and E_2 were (106.2±7.6)nmol/L,(210±16)nmol/L,(130±11)pmol/L in observation group 2 and(47.2±3.5)nmol/L,(130±6)nmol/L,(81±6)pmol/L in observation group 1,which also reach statistical difference(P<0.05).(2)The expression of enzyme:the expression of CYP17 mRNA was inereased by 50% and P450 arom mRNA was decreased by 20% between control group 1 and 2. However, the mRNA expression of P450 arom and CYPI7 were upregulated remarkably, especially, the expression of CYP17 mRNA were increased by 125%. Conclusion MAPK signaling pathway plays an inhibitory role in regulating synthesis of steroid hormone of ovarian granulose cell.
ABSTRACT
Aim To investigate the effect of cisplatin(CDDP) on toxicity and apoptosis of human luteinized granulose cells.Method Human luteinized granulose cells were prepared from oocyte retrieval of fertilization procedure in vitro and treated with various concentrations of cisplatin(0,0.5,1.0,2.5,5.0 mg?L-1).The inhibitory rate of cells was examined by MTT assay.The change of nucleolus stained with Hochest 33258 was observed under fluorescent microscope,and bcl-2 and bax protein expressions were detected by flow cytometry(FCM).Results The results showed that CDDP inhibited the growth of ovarian granulose cells at the concentration of no less than 1 mg?L-1,in which Hochest 33258 staining demonstrated cell apoptosis.The expression of bax protein increased as compared with that of the control(P