Research progress of growth differentiation factor-15 in disease of cardiovascular system / 中华检验医学杂志

Growth differentiation factor-15 (GDF-15) is a distant branch of the transforming growth factor-β (TGF-β) superfamily. Recent studies indicate that GDF-15 plays important physiological roles and has clinical implication in the initiation and development of various cardiovascular diseases. GDF-15 can both be used as a biomarker for the evaluation of cardiovascular disease occurrence and progression and a predictor for the risk stratification and long-term prognosis of cardiovascular diseases. The purpose of this review is to illustrate the latest research progress of GDF-15 in atherosclerosis and acute coronary syndrome obtained through the clinical research and basic experiments in cardiovascular diseases, to provide evidence for the selection of GDF-15 as useful biomarkers in the diagnosis and treatment of cardiovascular diseases.

L-PRF y Ciclo Celular. Revisión Narrativa / L-PRF and cell cycle. Narrative Review

RESUMEN: Los concentrados plaquetarios han emergido como un potencial material regenerativo, utilizado de forma aislada o como andamiaje para otros materiales de injerto. Son extractos de sangre, obtenidos después de procesar una muestra de sangre completa, mediante centrifugación. El primer reporte data de 1970, con un CP utilizado como pegamento para mejorar cicatrización de heridas de piel. En 1998, se usaron en cirugía oral y maxilofacial. Desde entonces, se han desarrollado diferentes técnicas y una variedad de preparaciones. Entre ellas, cabe destacar el plasma rico en plaquetas, fibrina rica en plaquetas y leucocitos (L-PRF) y plasma rico en factores de crecimiento (PRGF). El desarrollo de estos biomateriales, se debe en parte, a la posibilidad de alterar la concentración de mediadores químicos liberados en una lesión que provoque la formación de un coágulo, que pueda madurar conforme transcurran las fases del proceso inflamatorio y concluya con la regeneración íntegra del tejido dañado. El objetivo de este manuscrito fue describir las principales vías de señalización intracelular que se activan en presencia del L-PRF en cirugía oral, y sus efectos en la regulación del ciclo celular.

ABSTRACT: Platelet concentrates (PC) have emerged as a potential regenerative material, used in isolation or as scaffolding for other graft materials. They are blood extracts, obtained after processing a sample of whole blood, by centrifugation. The first report dates from 1970, with a PC used glue to improve the healing of skin wounds. In 1998, they were used in oral and maxillofacial surgery. Since then, different techniques and a variety of preparations have been developed. These include platelet-rich plasma, fibrin rich in platelets and leukocytes (L-PRF) and plasma rich in growth factors (PRGF). The development of these biomaterials, is due in part to the possibility of altering the concentration of chemical mediators released in a lesion that causes the formation of a clot, which can mature as the phases of the inflammatory process pass and conclude with the complete regeneration of the damaged tissue. The aim of this manuscript was to describe the main intracellular signaling pathways that are activated in the presence of LPRF in oral surgery, and its effects on the regulation of the cell cycle.

Correlation of serum growth differentiation factor-15 level with degree of coronary artery lesion and prognosis in elderly patients with acute coronary syndrome / 中华老年医学杂志

Objective@#To analyze the correlation of growth differentiation factor-15(GDF-15)level with the severity of coronary artery disease and prognosis and in elderly patients with acute coronary syndrome.@*Methods@#A total of 168 elderly patients with acute coronary syndrome admitted into Hangzhou Xixi Hospital and Shaoyifu Hospital Affiliated to Medical College of Zhejiang University from March 2017 to April 2018 were enrolled in the study.Patients were divided into three groups: GDF-15>1 800 μmol/L(n=28), GDF-15 between 1 200-1 800 μmol/L(n=45), and GDF-15<1 200 μmol/L(n=95). Plasma levels of high-sensitivity C-reactive protein(hs-CRP)and GDF-15, and SYNTAX score, single-vessel-, double-vessel, multi-vessel coronary disease and major adverse cardiovascular events(MACE)were analyzed and compared between groups.@*Results@#Plasma levels of hs-CRP and GDF-15 had significant differences among the three groups(P<0.05). There were significant statistically differences in SYNTAX score, single-vessel, double-vessel and multi-vessel coronary disease and MACE among the three groups(P<0.05). Logistic regression analysis showed that the levels of GDF-15(OR=2.092, 95%CI: 1.585~2.687), SYNTAX score(OR=1.948, 95%CI: 1.097~2.098), double-vessel lesions(OR=1.124, 95%CI: 1.006~1.251)and multi-vessel lesions(OR=1.744, 95%CI: 1.149~2.642)(all P<0.05)were statistically significant influencing factors for the prognosis.@*Conclusions@#GDF-15 level is correlated with SYNTAX score, double-vessel lesions and multi-vessel lesions, and could predict the severity of coronary artery lesions and prognosis in patients with acute coronary syndrome.

Correlation of serum growth differentiation factor-15 level with degree of coronary artery lesion and prognosis in elderly patients with acute coronary syndrome / 中华老年医学杂志

Objective To analyze the correlation of growth differentiation factor-15(GDF-15) level with the severity of coronary artery disease and prognosis and in elderly patients with acute coronary syndrome.Methods A total of 168 elderly patients with acute coronary syndrome admitted into Hangzhou Xixi Hospital and Shaoyifu Hospital Affiliated to Medical College of Zhejiang University from March 2017 to April 2018 were enrolled in the study.Patients were divided into three groups:GDF-15＞1 800 μmol/L(n=28),GDF-15 between 1 200-1 800 μmol/L(n=45),and GDF-15 ＜1 200 μmol/L(n =95).Plasma levels of high-sensitivity C-reactive protein (hs-CRP) and GDF-15,and SYNTAX score,single-vessel-,double-vessel,multi-vessel coronary disease and major adverse cardiovascular events(MACE)were analyzed and compared between groups.Results Plasma levels of hs-CRP and GDF-15 had significant differences among the three groups (P ＜ 0.05).There were significant statistically differences in SYNTAX score,single-vessel,double-vessel and multi-vessel coronary disease and MACE among the three groups(P＜0.05).Logistic regression analysis showed that the levels of GDF-15 (OR =2.092,95 ％ CI:1.585 ～ 2.687),SYNTAX score (OR =1.948,95 ％ CI:1.097 ～ 2.098),double-vessel lesions (OR =1.124,95％ CI:1.006 ～ 1.251) and multi-vessel lesions (OR =1.744,95 ％ CI:1.149 ～ 2.642) (all P ＜ 0.05) were statistically significant influencing factors for the prognosis.Conclusions GDF-15 level is correlated with SYNTAX score,doublevessel lesions and multi-vessel lesions,and could predict the severity of coronary artery lesions and prognosis in patients with acute coronary syndrome.

Complex of growth differentiation factor-5 and fibrin gel for repair of lumbar disc injury in rabbits / 中华创伤杂志

Objective To investigate the result of local injection of growth differentiation factor-5 (GDF-5) and fibrin gel for treatment of lumbar disc injury in rabbits.Methods Lumbar puncture with a 20-gauge needle was performed at L3/4,L4/5,and L5/6 discs of 40 New Zealand white rabbits.After needle puncture,L3/4 discs were injected with GDF-5 and fibrin gel (compound group) ; L4/5 discs fibrin gel (fibrin gel group) ; L5/6 discs nothing (blank control group).Two weeks later,intervertebral disc degeneration in each group was observed via radiography,MRI and nucleus proteoglycan content detection and histological examination.Results At postoperative 2-,4-,8-,and 12-weeks,X-ray films revealed a gradual decrease in disc height index (DHI) among the three groups,but the decreasing velocity was lower in compound group than in other two groups (P ＜ 0.05).On MRI,the signal of intervebral discs among the three groups diminished progressively with time,but a relatively lower decreasing was observed in compound group (P ＜ 0.05).At postoperative 4-week,proteoglycan content of the nucleus pulposus was (6.3-± 0.4) in compound group,higher than (5.9-0.4) in blank control group and (5.8-± 0.3)in fibrin gel group (P ＜0.05).At postoperative 2-week,histological evaluation showed (5.28 ±0.41)points in compound group,lower than (7.54 ± 0.53) points in blank control group and (7.21 ± 0.44)points in fibrin gel group (P ＜ 0.05).Conclusion Local injection of GDF-5 and fibrin gel facilitates the restoration of the injured discs and delays further disc degeneration.