ABSTRACT
Objective: To study the treatment effect of Qingyan Droping Pills (QDP) for oral ulcer. Methods: SPF level 60 rats were randomly divided into control group, model group, positive control (Guilin Watermelon Frost 200 mg/kg) group, high-, mid-, and low-dose QDP groups (n = 10). The oral ulcer model of rat was established by injection of acetic acid on the oral mucosa of left cheek in rat, at 24 h immediately after the formation of ulcers. The first two days after the start of the administration modeling, continuous administration of 10 d was performed. The ulcer diameters before the administration and 4, 7, 10 d after administration were recorded by vernier caliper. Rats in each group after the last measurement were sacrificed and part of oral ulcers were removed, some of ulcers tissue were homogenized by TNF-α assay kit. The remaining ulcers were sliced and the ulcer morphology was observed. Results: Mouth ulcer areas of high-dose QDP group and Watermelon Frost group were significantly reduced (P < 0.05, 0.01), ulcer tissue lesions and TNF-α levels were significantly reduced (P < 0.01). Conclusion: QDP on acetic acid-induced oral ulcers has some therapeutic effect.
ABSTRACT
AIM: To establish an HPLC-MS method for simultaneously determining four effective components in Guilin Watermelon Frost(Mirabilitum Praeparatum,Rhizoma Coptidis,Radix Scutellariae,etc.). METHODS: The sample was extracted with methanol containing 20% chloroform under ultrasonication.The HPLC separation was performed on Zorbax SB C_(18)(3.0 mm?250 mm,5 ?m) column using water including 0.5% formic acid(A)-methanol(B) as mobile phase,with the gradient elution(0-2 min,60%B;2-5 min,60%→90%B;5 min to the end,90%B), at a flow rate of 0.40 mL/min.The compounds were analyzed by ESI-MS under ion monitoring mode(0-3 min,m/z 249;3-6 min,m/z 336;6-10 min,m/z 447;10-16 min,m/z 263). RESULTS: The linear ranges were 0.020-10.0 ?g/mL,0.010-40.0 ?g/mL,0.036-50.0 ?g/mL and 0.040-4.00 ?g/mL for matrine,berberine,baicalin and indirubin,with detection limits of 0.005,0.001,0.006 and 0.010 ?g/mL,respectively.The average recoveries ranged from 96% to 101% with all relative standard deviations less than 3%.CONCLUSION: (The method is rapid,accurate and suitable for the quality control of the four effective) components in Guilin Watermelon Frost.