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Objective: To study the toxicity alleviation effect of Bovis Calculus Artificialis, Gypsum Fibrosum, and Borneolum Synthcticum to Realgar in Niuhuang Jiedu Tablet based on 1H-NMR metabonomics. Methods: Forty-two male Wistar rats were divided into six groups: group A (control group), group B (treated with realgar), group C (treated with Niuhuang Jiedu Tablet), group D (treated with Niuhuang Jiedu Tablet without Bovis Calculus Artificialis), group E (treated with Niuhuang Jiedu Tablet without Gypsum Fibrosum), and group F (treated with Niuhuang Jiedu Tablet without Borneolum Synthcticum). Based on 1H-NMR spectra of urine and serum from rats, PLS-DA was performed to identify different metabolic profiles. Results: The metabolic profiles of group B were different from control group, while the metabolic profiles of all other groups were almost in line with those of control group. Conclusion: The toxicity alleviation effects of Bovis Calculus Artificialis, Gypsum Fibrosum, and Borneolum Synthcticum to Realgar in Niuhuang Jiedu Tablet are not obvious.
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Gypsum Fibrosum is one of mineral medicines, which is widely used in clinics for a long time. It is mainly used for treating epidemic febrile diseases and excess-heat syndrome. The studies on the heat-clearing effects and mechanisms of Gypsum Fibro-sum in recent years were summarized in the paper,which can provide scientific reference for its further research, application and the heat-clearing mechanism exploration.
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Objective:To analyze the characteristic peaks of gypsum fibrosum from authentic product area Hubei Yingcheng via X-ray diffraction. Methods:The fingerprint and common peaks of gypsum fibrosum from different areas were analyzed via X-ray diffrac-tion, and the characteristic peaks of gypsum fibrosum from authentic product area Hubei Yingcheng were identified Results:The finger-prints of X-ray diffraction of gypsum fibrosum from different areas were obtalned. Sixteen common peaks were determined, and two characteristic peaks of gypsum fibrosum from authentic product area Yingcheng were determined via X-ray diffraction with interplanar spacing of 1. 68 and 1. 51, respectively. Conclusion:The determination of two characteristic peaks of gypsum fibrosum from authentic product area Hubei Yingcheng can distinguish gypsum fibrosum from the other areas and provide basis for the quality evaluation of gyp-sum fibrosum.
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Objective:To investigate gypsum fibrosum from different habitats by scanning electron microscopy ( SEM) . Methods:Gypsum fibrosum samples from different habitats were scanned by an electronic microscope. Results: The results of SEM showed that gypsum fibrosum (CaSO4·2H2O) from different habitats was with the crystal structure of fibrous,tabular or flaky aggregation. The sur-face amplification of gypsum fibrosum from Hubei showed obvious black spots,while that from the other habitats was without the feature. Conclusion:The method is simple and accurate, which can be used to investigate gypsum fibrosum from different habitats.
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Objective To establish a method for the determination of the dissolution rate of CaSO4 ·2H2 O in Gypsum Fibrosum. Methods The dissolution rate was determined by EDTA-2Na titration method,with a paddle,800 mL of purified water as dissolution medium,at rotation speed of 75 r·min-1,and temperature of(37. 0±0. 5 ) ℃,at 5,10,20,30,60,90, 120 min for the sampling. Results There was a linear regression relationship between dissolution time and dissolution of CaSO4 ·2H2 O,when the particle size of Gypsum Fibrosum were 2. 000, 0. 150 mm. There was a logarithmic regression relationship between dissolution time and dissolution of CaSO4 ·2H2 O,when the particle size of Gypsum Fibrosum was 0. 075 mm. Conclusion The method is simple and accurate,which can be used for determination of the dissolution rate of CaSO4 · 2H2 O in Gypsum Fibrosum.
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Objective To study the effects of Chinese herb extracts on cell proliferation and glucose absorption in intestinal epithelial cell line(IEC-6 cells) of rats.Methods The extracts of four Chinese herbs,including Herba Agastaches(HA),Rhizoma Atractylodis(RA), Cortex Phellodendri(CP),and Gypsum Fibrosum(GF),were made.Their suitable concentration on the cell proliferation in IEC-6 cells was determined by MTT method,and glucose absorption and the activity of Na+,K+-ATPase in IEC-6 cells were assayed.A method of real time PCR was applied to the determination of SGLT1 and GLUT2 mRNA expression in the cells.Results Chinese herb extracts treatment altered the cell proliferation in a dose-dependent manner.Moreover,RA volatile oil(50 ?g/mL) and CP alkaloid(10 ?g/mL) treatment increased glucose absorption and the activity of Na+,K+-ATPase genes(P0.05).Conclusion The three extracts treatment,including RA volatile oil,CP aklaloid,and HA volatile oil,could increase glucose absorption and the expression of glucose transport carrier genes,but their regulative mechanism are not totally the same.
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Objective: To provide experiment data for ultra fine prepared mineral drugs Methods: Electron microscope scannin,X ray diffraction were used in identification, atomic emit sectrum was used in determination. Results: The dissolution rate of ca 2+ composition could be high.The ultra fine prepared of minaral drugs could be prepared with powder diameter of K 4。 Conclusion: Ultra fine Prepared fluoritum and gypsum fibrosum may Save clinically dose.