Prevalence of Helicobacter pylori infection among asymptomatic children in southeastern Brazil: a cross-sectional study

ABSTRACT BACKGROUND: The prevalence of Helico bacter pylori (H. pylori) infection is decreasing worldwide, but is still high in developing countries. We previously observed an H. pylori infection rate of 52% among children and adolescents with chronic non-ulcer dyspepsia. OBJECTIVE: To investigate the prevalence of H. pylori infection among asymptomatic children living in a single region and to evaluate the risk factors for this infection. DESIGN AND SETTING: Cross-sectional study in which 161 children aged 5-13 years (mean age 7.8 years), at a public school in Botucatu, state of São Paulo, southeastern Brazil, were assessed. METHOD: The children's H. pylori infection status was determined through the urea breath test and the risk factors for acquisition of the infection were determined based on a sociodemographic questionnaire. RESULTS: The overall prevalence of H. pylori infection was 20.5%: 18.7% among females and 22.2% among males. The results from the sociodemographic survey did not differ between children with and without H. pylori infection. 30.9% of the children had previous records of upper gastrointestinal symptoms, which consisted of H. pylori infection in only 26.5% of these cases. Family histories of gastritis and peptic ulcer disease were found in relation to 50% and 32.3% of the children with H. pylori infection respectively. CONCLUSION: The prevalence of H. pylori infection among asymptomatic children in southeastern Brazil is lower than that recorded among symptomatic children in the same region and similar to the prevalence of H. pylori infection observed in developed countries.

Effect of H. Pylori on Fibrinogen Level among Sudanese Patients at Khsartoum State

Background: Helicobacter pylori (H. pylori) is a gram-negative, spiral shaped pathogenic bacterium that specifically colonizes the gastric epithelium causing chronic gastritis, peptic ulcer disease, and/or gastric malignancy.Aim: To assess the effect of H. pylorion fibrinogen level of Sudanese patients with diabetes millets (D.M) living in Khartoum state.Subjects and Methods: This was case control study conducted among Sudanese diabetic patients during the period from May 2018 to July 2021. A total of 181 diabetics patients are involved in this study. Citrated blood and stool samples were collected from each participant. All stool samples were tested for the presence of H. pylori antigen by using commercially available H. pylori antigen detection card. Based on the result examination for H. pylorithe study population are sub grouped in two groups one group 124 of cases with Positive H. pylori antigen and another group 57 of controls with negativeH. pylori infection. Both groups were assessed for the fibrinogen level. Data was gathered and analyzed by using SPSS version 20.Results: Our results revealed that cases have slightly higher values of fibrinogen levels 326.29± 89.99 mg/dl compared to 309.47± 90.82 mg/dl for control group. However, the statistical analysis indicate that the difference was remain insignificant with P-value of 0.246. Among cases group gender concerned the fibrinogen concentration mean in the plasma of male 336.2±98.5 mg/dl was higher than female 311.1±73.4 mg/dl.Conclusion:they were insignificant association between fibrinogen level and presence of H. pyloriinfection among Sudanese diabetic patients. gender concerned the fibrinogen concentration in the plasma in H. pylori among males showed higher levels than females.

Effect of H. pyloriamong Diabetic Patients Living in Khartoum State, Sudan

Background:Studies suggest an association between Helicobacter pylori (H. pylori)infection and extra gastrointestinal disease. Limited studies provided conflicting results on the association between H. pyloriinfection and diabetes. The present study was aimed at examining the association between H. pyloriinfection and diabetes among Sudanese patients.Methods:This was case control study conducted among Sudanese diabetic patients during the period fromMay 2018 to July 2021. A total of 181 diabetics patients are involved in this study. Blood, stool samples were collected from each participant. All stool samples were tested for the presence of H. pylori antigen by using commercially available H. pyloriantigen detection card. Based on the result examination for H. pylori the study population are sub grouped in two groups one group 124 of cases with Positive H. pyloriantigen and another group 57 of controls with negative H. pylori infection. Both groupswere assessed for the level of fasting blood glucose. Data was gathered and analyzed by using SPSS version 20.Results: among cases group the mean of fasting blood glucose was 201.94± 90.45 mg/dl compared to 199.40± 97.83 mg/dl among control group. The statistical analysis was showed insignificant difference between cases and controls in the level of fasting blood glucose with P-value of 0.864 . In addition, among cases group the mean of male’s blood glucose 213.0±91.52 mg/dl was higher than females blood glucose level 185.0 ±87.0 mg/dl.Conclusion: They were insignificant association between blood glucose level and H. pylori infection among diabetes patents, However, the subgroup analysis suggested that H. pylori infection was possibly associated with increased risk of diabetes among males. Future cohort studies are needed to verify this association in females and to address possible implication in the prevention of diabetes

Helicobacter pylori infection and its correlates among the patients of peptic perforation and gastric carcinoma

Background: Presence of H. pylori infection was found associated with peptic perforation and gastric carcinoma. Present study tried to estimate the prevalence of H. pylori infection in those patients and to find out the correlates of H. pylori infection.Methods: After matching the inclusion and exclusion criteria, all cases of diagnosed peptic perforation and gastric carcinoma were taken for this prospective, single center, observational study.Results: Among the study population, gastric carcinoma was found in higher age group; whereas peptic perforation was found in lower age group; male and female ratio was 2:1 in both groups of patient. Laborer and housewives were mostly affected in both cases. Gastric carcinoma was more prevalent in urban residents, opposite was seen in peptic perforation. Most patients in two groups had no previous co-morbid condition. Use of NSAIDs was found in high frequency in both groups. Most of the patients were chronic alcoholic and chronic smoker, and most of them had history of taking spicy foods more than twice in a week.Conclusions: H. pylori infection was found in high frequency in both group of patients, and it was higher in peptic perforation. The study establishes the association of H. pylori with the gastric carcinoma and peptic perforation.

Is Helicobacter pylori Infection Associated with Perforated Gastro-Duodenal Ulcer

About 95% of the patients suffering from duodenal ulcer and 70-80% of the gastric ulcer patients are found to be Helicobacter pylori (H. pylori) positive. Although the role of H. pylori is well known in peptic ulcer disease aetiology, its role in perforated peptic ulcer is not well established. There are conflicting results in the literature regarding its association, with some studies showing high prevalence of H. pylori infection in gastro-duodenal perforation patients and also its eradication prevented the relapse of ulcer while others showing low or lack of association, suggesting a different pathogenesis for perforated peptic ulcer.METHODSGroup-I patients were operated for gastro-duodenal perforation and group-II patients were with the upper gastrointestinal endoscopic diagnosis of peptic ulcer disease. The mucosal biopsy samples taken from antrum from both the groups were tested with rapid urease test and histopathological examination to detect H. pylori.RESULTSThe prevalence of H. pylori infection was significantly less in patients with perforated gastro-duodenal ulcer than with peptic ulcer disease (p-value-0.026; OR – 0.31).CONCLUSIONSThrough this study it was found that alcohol intake and smoking were significant risk factors associated with perforation of gastro-duodenal ulcer whereas NSAIDs use was not significantly associated. H. pylori infection was not significantly associated with perforated gastro-duodenal ulcer suggesting that some other factors were involved in perforation.

Risk Factors Analysis of Gastro- Intestinal Infections among the Patients in Tertiary Care Hospital in Dhaka, Bangladesh

Background: Viral hepatitis is one of the major public healthconcerns around the world. Every year millions of people diefrom viral hepatitis-related cirrhosis and liver cancer. Howeverthe fact is the majority of the infected populations are unawareof their condition.Objective: The objective of the study was to find out theproportion of the risk factors associated with gastrointestinalinfections among the inpatients attending in tertiary carehospital in Dhaka city.Materials & Methods: A cross-sectional study was carried outamong the hospital admitted patients at gastroenterologydepartment from January to April, 2019.A total of 193patientswere purposively selected who were above 18 years anddiagnosed case of hepatitis, diarrhoeal diseases, enteric fever,H. pylori infection.Results: The outcome of the study was that, majority82(42.5%) of the patients age was between 21-40 years.Maximum participants were male (65.3%). Study found thatamong 193 patients, 45 (23.3%) were diagnosed as hepatitisB, 31 (16.1%) were H. pylori infection, 29 (15.0%) diarrhoea,hepatitis C 19 (9.8%), hepatitis E 23 (11.9%) and hepatitis A 21(10.9%). It also found that mean duration of Gastro-Intestinal(GI) infection was 2.59 (± 3.22) years. Water borne diseaseslike hepatitis A, E, diarrhoea, typhoid and H. pylori infectionmostly presents due to 44 (34.4%) patients drunk water fromsupply water (Dhaka WASA), 73.4% eaten street juice and79.7% had eaten street food before diagnosed diseases.Commonest risk factors were hepatitis B and C. 37 (56.9%)patients had received blood previously, 11 (17.2%) had theirprevious history of operation, 42 (64.6%) had visited dentist fordental issues, 29 (44.6%) patients attended endoscopy orcolonoscopy test, 28 (43.1%) patients informed that they hadfaced minor skin injury when shaving in salon and 6 (9.2%)patients mentioned taken dialysis. According to the findings,20.3% patients having liver diseases, 5.7% heart diseases,4.1% respiratory diseases, 7.3% renal diseases, 26.4%diabetic, 22.8% hypertensive, 15.0% anemia and 26.9%smoker Street juice [OR: 95% CI, 4.9%] and Street food [OR:95% CI, 6.1%] risk of hepatitis A infection. The proportion ofrisk factors of hepatitis B infection revealed that the patientswho received blood previously [OR: 95% CI, 3.14%] has therisk of hepatitis, second hand razor use [OR: 95% CI, 7.76%],Dialysis [OR: 95% CI, 2.47%].Conclusion: Street foods & juice, unpurified water was thecommonest factors of hepatitis A &E, typhoid and diarrhea. Onthe other side, unscreened blood, dental procedure, skin injuryin saloon, dialysis and endoscopy/colonoscopy test were thecommonest risk of hepatitis B& C virus. H. pylori infection

Efficacy and safety of rifabutin and furazolidone included triple regimen and minocycline plus furazolidone included quadruple regimen in eradicating refractory Helicobacter pylori infection / 中华消化杂志

Objective To observe the efficacy and safety of rifabutin and furazolidone included triple regimen and minocycline plus furazolidone included quadruple regimen in eradicating refractory H.pylori infection.Methods A total of 146 patients who failed (≥ two times) to treat H.pylori with standard antiH.pylori therapy were selected and divided into esomeprazole,rifabutin and furazolidone treatment group (ERF group,n =74) and esomeprazole,minocycline,furazolidone and bismuth potassium citrate group (EMFB group,n =72).The duration of treatment were both 10 days.Liver and renal functions were examined within three days after therapy.13C or 14 C-urease breath test was performed one month after the medication withdrawal.The patients were followed up once every two weeks during the treatment period.The medication and adverse reactions were recorded in detail.The compliance and rates of adverse events in two groups were compared and analyzed.The eradication rates of the two groups and subgroups were compared by intention-to-treat (ITT) and per-protocol (PP) analyses.The cost-effectiveness of the two groups was evaluated with cost effectiveness analysis (CEA).The cost/effectiveness (C/E) ratio was calculated by PP.Chi-square test and t test were used for statistical analysis.Results There was no significant difference in complicance between ERF group and EMFB group (90.5％ vs.90.3％,P ＞0.05).There was no significant difference in H.pylori eradication rate between ERF group and EMFB group analyzed by ITT (82.4％ vs.84.7％,P ＞0.05).Analyzed by PP,there were no significant differences in H.pylori eradication rate between ERF group and EMFB group (91.0％ vs.93.8％),between male subgroup and female subgroup of ERF group (87.9％ vs.94.1％),between subgroup with age less than 60 years and subgroup with age over 60 years of ERF group (89.7％ vs.92.9％),between male subgroup and female subgroup of EMFB group (89.7％ vs.97.2％) and between subgroup with age less than 60 years and subgroup with age over 60 years of EMFB group (93.6％ vs.94.4％) (all P ＞ 0.05).There were no statistically significant differences in the rate of adverse events between ERF group and EMFB group (20.3％ vs.22.2％),between male subgroup and female subgroup of ERF group (25.0％ vs.15.8％),between subgroup with age less than 60 years and subgroup with age over 60 years of ERF group (26.2％ vs.12.5％),between male subgroup and female subgroup of EMFB group (19.4％ vs.24.4％) and between subgroup with age less than 60 years and subgroup with age over 60 years of EMFB group (24.5％ vs.15.8％)(all P ＞ 0.05).Fixed daily cost,total cost and C/E of ERF group was RMB 59.0 yuan,RMB 590.5 yuan and 648.9,respectively;and the eradication rate was 91.0％ (61/67).Fixed daily cost,total cost and C/E of EMFB group was RMB 32.9 yuan,RMB 329.1 yuan and 350.9,respectively;and the eradication rate was 93.8％ (61/65).Conclusions Rifabutin and furazolidone included triple regimen and minocycline plus furazolidone included quadruple regimen both have good efficacy,safety and compliance in the eradication of refractory H.pylori infection,and the latter is better.

Efficacy and safety of rifabutin and furazolidone included triple regimen and minocycline plus furazolidone included quadruple regimen in eradicating refractory Helicobacter pylori infection / 中华消化杂志

Objective@#To observe the efficacy and safety of rifabutin and furazolidone included triple regimen and minocycline plus furazolidone included quadruple regimen in eradicating refractory H. pylori infection.@*Methods@#A total of 146 patients who failed (≥two times) to treat H. pylori with standard anti-H.pylori therapy were selected and divided into esomeprazole, rifabutin and furazolidone treatment group (ERF group, n=74) and esomeprazole, minocycline, furazolidone and bismuth potassium citrate group (EMFB group, n=72). The duration of treatment were both 10 days. Liver and renal functions were examined within three days after therapy. 13C or 14C-urease breath test was performed one month after the medication withdrawal. The patients were followed up once every two weeks during the treatment period. The medication and adverse reactions were recorded in detail. The compliance and rates of adverse events in two groups were compared and analyzed. The eradication rates of the two groups and subgroups were compared by intention-to-treat (ITT) and per-protocol (PP) analyses. The cost-effectiveness of the two groups was evaluated with cost effectiveness analysis (CEA). The cost/effectiveness (C/E) ratio was calculated by PP. Chi-square test and t test were used for statistical analysis.@*Results@#There was no significant difference in complicance between ERF group and EMFB group (90.5% vs. 90.3%, P>0.05). There was no significant difference in H. pylori eradication rate between ERF group and EMFB group analyzed by ITT (82.4% vs. 84.7%, P>0.05). Analyzed by PP, there were no significant differences in H. pylori eradication rate between ERF group and EMFB group (91.0% vs. 93.8%), between male subgroup and female subgroup of ERF group (87.9% vs. 94.1%), between subgroup with age less than 60 years and subgroup with age over 60 years of ERF group (89.7% vs. 92.9%), between male subgroup and female subgroup of EMFB group (89.7% vs. 97.2%) and between subgroup with age less than 60 years and subgroup with age over 60 years of EMFB group (93.6% vs. 94.4%) (all P>0.05). There were no statistically significant differences in the rate of adverse events between ERF group and EMFB group (20.3% vs. 22.2%), between male subgroup and female subgroup of ERF group (25.0% vs. 15.8%), between subgroup with age less than 60 years and subgroup with age over 60 years of ERF group (26.2% vs. 12.5%), between male subgroup and female subgroup of EMFB group (19.4% vs. 24.4%) and between subgroup with age less than 60 years and subgroup with age over 60 years of EMFB group (24.5% vs. 15.8%) (all P>0.05). Fixed daily cost, total cost and C/E of ERF group was RMB 59.0 yuan, RMB 590.5 yuan and 648.9, respectively; and the eradication rate was 91.0%(61/67). Fixed daily cost, total cost and C/E of EMFB group was RMB 32.9 yuan, RMB 329.1 yuan and 350.9, respectively; and the eradication rate was 93.8%(61/65).@*Conclusions@#Rifabutin and furazolidone included triple regimen and minocycline plus furazolidone included quadruple regimen both have good efficacy, safety and compliance in the eradication of refractory H. pylori infection, and the latter is better.

Association of Helicobacter pylori with Parkinson's Disease

BACKGROUND AND PURPOSE: Parkinson's disease (PD) is a major neurological disorder that requires lifelong treatment, and the combined presence of Helicobacter pylori (H. pylori) infection can increase the required anti-PD medications. We aim to investigate the effect of H. pylori infection in Indian PD patients. METHODS: We prospectively recruited 36 PD patients from December 2007 to January 2011. All patients underwent a detailed neurological evaluation and serological examination for H. pylori infection. Seropositive and seronegative patients were considered to be the cases and controls, respectively. All patients who were seropositive received triple therapy for 2 weeks. Outcome measures of the mean ‘off’ Unified Parkinson's Disease Rating Scale (UPDRS)-III score, mean ‘on’ UPDRS-III score, mean onset time, mean ‘on’ duration, and mean daily ‘on’ time were measured at baseline and at a 3-week follow-up. RESULTS: H. pylori-IgG positivity was present in 18 (50%) PD patients. The prevalence of men (72.2% vs. 33.3%), mean duration of disease (13.8 vs. 12.5) and mean levodopa equivalent daily dose (824 mg vs. 707 mg) were significantly higher among H. pylori positive patients than in controls (p<0.0001). Controls had a significantly longer ‘on’ duration and daily ‘on’ time, and better ‘on’ UPDRS-III scores. Seropositive patients took a significantly longer time to turn ‘on’ after a levodopa challenge. At the 3-week follow-up, H. pylori eradication significantly improved the mean ‘on’ UPDRS-III score, onset time, ‘on’ duration, and daily ‘on’ time. CONCLUSIONS: H. pylori infection was present in 50% of Indian PD patients. H. pylori seropositivity was associated with a poor response to levodopa and increased medication usage, while eradication therapy was associated with better patient outcomes.

Preliminary analysis of association between stomach cracks under gastroscopy and h.pylori infection and its pathological significance / 中国综合临床

Objective To investigate the clinical significance of gastric cracks by investigating the relationship between stomach cracks under gastroscopy and H.pylori infection as well as its pathological manifestation,in order to improve the detection rate of H.pylori.Methods Gastroscopy patients were enrolled as our subjects excluding those with tumors,major diseases and under 18 years old.Mucosal biopsies were obtained from the lesser curvature of antrum and the greater curvature of body in each patient,then by HE staining,pathological and HP examination.The information including the pathological diagnosis,HP antibody,rapid urease test,endoscopic diagnosis and endoscopic gastric body cracking phenomenon were collected from all patient.H.pylori positivity was defined as a patient who showed two or more positive results in the Rapid Urea's Test,pathohistological examination and H.pylori antibody detection.Results (1) 437 patients with gastroscopy were included between December 2009 and December 2011,among whom 210 were male and 227 were female.There were 32 cases with gastric ulcer,59 cases with duodenal ulcer,51 cases with duodenitis,62 cases with reflux esophagitis,and 276 cases with chronic gastritis.(2) H.pylori distribution in stoma of all patients:160 of 437 patients(36.61％) are H.pylori positive,of which 68.75％ (110/160) was found H.pylori positive in both of gastric antrum and body,25.63％ (41/160) was H.pylori positive only in antrum,and 3.13％ (5/160) was H.pylori positive only in the body of stomach.(3)Relationship between the presence of cracks in gastric body and H.pylori infection:the H.pylori positivity was 62.58％ (97/155) and 22.34％ (63/282) respectively in the group with or without the presence of cracks in body.Cracks in gastric body examined by gastroscopy was related to H.pylori infection(x2 =69.788,P =0.000).(4) Relationship between the presence of cracks in gastric body and patho-histology:the presence of cracks in gastric body was related with severity of gastric inflammation by Wilcoxon rank sum test (P ＜ 0.0001),while there was no relationship between gastric body cracks and atrophy,intestinal metaplasia and atypical hyperplasia(P ＞ 0.05).(5) Relationship between the presence of cracks in gastric body and diseases:the presence of cracks in body was related with duodenitis,bile reflux under gastroscopy while there was no difference among in gastric body and gastric ulcer,esophagitis and chronic gastritis in terms of cracking appearance (P ＞ 0.05).Conclusion Our finding showed that cracking appearance in the gastric body is related with severity of inflammation in gastric mucosa bile reflux and duodenitis,suggesting that it can be one of the gastroscopic manifestations of more severe gastritis.

Gastric Helicobacter Pylori Infection / Peptic Ulcers And Periodontal Disease: A Literary Overview.

H.Pylori is an important factor in pathogenesis of numerous diseases including metabolic, gastrointestinal and vascular disorders.The oral cavity has been proposed as a reservoir for Helicobacter pylori that could be responsible for refractoriness of gastric infection to triple therapy (antibiotics, antimicrobials, proton pump inhibitors). Analysis of data suggests that periodontal treatment in combination with systemic therapy could be a promising approach to increasing the therapy’s efficacy and decreasing the risk of infection recurrence. Some authors have given conflicting evidences, to date there has been no clear cut evidence / study which directly correlates H. Pylori infection of periodontal pockets / plaque leading to peptic ulcer, duodenal ulcers and stomach cancers.

Organic Upper Digestive Diseases in Children with Chronic Abdominal Pain / 대한소아소화기영양학회지

Organic diseases are prevalent in about 5 to 10% of children with chronic abdominal pain. The most common diseases of the upper digestive tract include gastroesophageal reflux disease (GERD), chronic gastritis with or without Helicobacter pylori (H. pylori), and peptic ulcer. The H. pylori infections acquired during childhood persist lifelong without eradication. Although the majority of H. pylori infected children remain asymptomatic, H. pylori infection may cause various digestive and extra-digestive diseases. There are still debates about a causal relationship between H. pylori-gastritis and abdominal symptoms in the absence of peptic ulcer disease. The number of Korean children infected with antibiotic resistant H. pylori is increasing even though the prevalence decreases after eradication. The choices of rescue therapy are limited in children after eradication failure. Antioxidant supplements with regimens against H. pylori have been tried with limited effects. Here I wanted to review the findings of recent reports on common upper digestive diseases such as GERD, peptic ulcer, and H. pylori infection in children with chronic abdominal pain.

The assessment of probable relationship between lung cancer and Helicobacter pylori infection.

Background: Many sero-epidemiological and case-control studies suggest that H.pylori infection may be associated with the development of lung cancer. Aim: The objective of this study was to investigate the relation between lung cancer and Helicobacter pylori infection. Methods: 66 consecutive patients with histologically confirmed, primary lung cancer and 66 controls were enrolled in this study. All enrolled subject underwent an enzyme-linked immunosorbent assay (ELISA) IgG serologic test for H. pylori diagnosis. Results: The study included 66 histologically verified lung carcinoma (53 men and 13 women) with the median age of 59 years (range 30 to 87 years) and 66 controls (50 men and 16 women) with the median age of 58 years (range 27 to 88 years). The prevalence of H.pylori seropositivity was 73% (48/66) in lung cancer but only 51% (34/66) in controls. The odds ratio for the association of H.pylori and lung cancer was 2.51 (95% CI 1.14 – 5.54, P<0.05). Conclusion: The results of our study show that the population of patients with lung cancer has a significantly higher rate of seropositivity for antibodies against H.pylori than the population of subjects without lung cancer.

ABO blood groups and Helicobacter pylori cagA infection: evidence of an association

Diseases resulting from Helicobacter pylori infection appear to be dependent on a host of genetic traits and virulence factors possessed by this microorganism. This paper aimed to investigate the association between the ABO histo-blood groups and H. pylori cagA infections. Genomic DNA samples (n = 110) of gastric biopsies obtained from patients with endoscopic diagnosis of peptic ulcers (n = 25) and chronic active gastritis (n = 85) were analyzed by PCR using specific primers for the cagA gene. Of the samples, 66.4 percent (n = 73) tested positive and 33.6 percent (n = 37) negative for the gene. The cagA strain was predominant in peptic ulcers (n = 21; 84.0 percent) compared with chronic active gastritis (n = 52; 61.2 percent) (p = 0.05; OR 3.332; 95 percent CI: 1.050-10.576). Additionally, the cagA strain was prevalent in the type O blood (48/63; 76.2 percent) compared with other ABO phenotypes (25/47; 53.2 percent) (p = 0.01; OR 2.816; 95 percent CI: 1.246-6.364). These results suggest that H. pylori cagA infection is associated with the O blood group in Brazilian patients suffering from chronic active gastritis and peptic ulcers.

Validity of 38 mg Low Dose 13C-Urea Breath Test for the Diagnosis of Helicobacter pylori Infection

BACKGROUND: To assess the diagnostic value of 13C-UBT using capsulated 38 mg low dose for H. pylori infection, we investigated the sensitivity and specificity of 13C-UBT and to suggest the optimal positive cut-off value of DOB20 in 13C-UBT using ROC analysis. METHODS: The study subjects were 76 healthy individuals (males; 52, females; 24) who visited a health promotion center at a university hospital between July 2005 and June 2007. We tested for H. pylori infection by 38 mg low dose 13C-UBT and histology. We measured the expiratory 13C-urea concentration of basal and 20 minutes value after oral ingestion of 38 mg 13C- labelled urea with capsulated. The breath samples were analysed by gas chromatograph isotope ratio mass spectrometer and expressed as units of delta. RESULTS: Fifty subjects (65.8%) were H. pylori positive as judged from histology. ROC analysis showed an area under the curve (AUC) of 0.943 (95% confidence interval: 0.891~0.995), indicating an excellent classification performance of the model. The sensitivity of 96%, specificity of 85% were achieved at the optimal cut-off value of DOB20 which was 1.39. The 38 mg low dose 13C-UBT was a non-invasive, simple, short-time required and highly accurate method. CONCLUSION: The results suggested that capsulated 38 mg low dose 13C-UBT is considered more in term of accuracy, costeffectiveness and convenient method for the diagnosis of H. pylori infection. Further long-term research and meta analysis based on large-scale investigations is needed to establish a standardized testing method in creating protocol of 13C-UBT.

Treatment of Helicobacter pylori infection / 대한내과학회지

No abstract available.

Helicobacter pylori infection and abdominal pain in children / 소아과

The relationship between H. pylori(Hp) infection and recurrent abdominal pain(RAP) in children is not clear. Current data in the literature regarding a causal relationship between Hp infection and childhood RAP are conflicting. However, meta-analysis and most of the recently published studies have not supported an association between Hp infection and an increased prevalence of abdominal pain. Most published studies have some problems and have been criticized on methodological grounds. Most of the existing studies have not been designed to include double-blind placebo controls. And, because of the low incidence of Hp infection in children, they have a problem of obtaining sufficient numbers of the Hp infected children. There is also a concern about the heterogeneity of testing methodology in the studies and diagnostic accuracy problems of the non-invasive tests such as low sensitivity of the serology tests and false positive results of the UBT in children. And conflicting results may be explained, in part, by the poor definition of RAP or dyspepsia for the study subjects. To date, no consensus exists for the treatment of Hp-infected children with RAP. Some authors suggest the clinical efficacy of Hp. But others suggest that eradication of Hp is not associated with improvement of RAP. The problem of the most treatment studies is that they had usually no control groups and the number of the subjects was small. In order to make reasonable decisions about treatment of Hp infection in children with RAP, long-term, randomized, double blind, well controlled studies in large numbers of children will be required.

The Seroprevalence of IgM and IgG Antibodies to Helicobacter pylori Among Maternal, Neonatal, and Cord Bloods in Korea / 임상검사와정도관리

BACKGROUND: Helicobacter pylori (H. pylori) infection has been known closely related with gastritis, duodenal ulcer and gastric cancer and is prevalent among Koreans. However, the infection route and the time are unclear, especially during perinatal period. The aim of this study is to investigate the relationship of H. pylori IgG and IgM antibody prevalences and titers between maternal, neonatal, and cord blood. METHODS: We collected 45 simultaneous maternal, neonatal, and cord bloods and 150 single cord bloods during delivery. The specific H. pylori IgG and IgM antibody levels were measured by enzyme-linked immunosorbent assay (ELISA) method. RESULTS: The H. pylori IgG antibody-positive rate for maternal, neonatal, and cord bloods were equal as 35.6% (16/45). The H. pylori IgG antibody levels of neonatal and cord bloods were 52.7% and 70.7% of maternal blood level. The H. pylori IgG antibody levels between maternal and cord bloods (r2 = 0.9725, p<0.05), maternal and neonatal bloods (r2 = 0.8569, p<0.05), and neonatal and cord bloods (r2 = 0.9437, p<0.05) were well correlated. Only one case of maternal blood was H. pylori IgM antibody positive and it's antibody level was 52.3 U/mL. CONCLUSIONS: In this study, we provided the sero-prevalence of H. pylori IgG and IgM antibodies and the relationship of antibody level of H. pylori IgG in maternal, neonatal and cord bloods. To elucidate the exact route and time of H. pylori infection, further studies including serial measurement of H. pylori IgG and IgM level in neonates will be needed.

Enhancing the value of biposy urease test by two biposy specimen collection in the diagnosis of H. pylori infection on peptic ulcer disease patient

200 patients over 18 years old were diagnosed with peptic ulcer. Two specimens were taken on each patient: from the corpus and 1/3 andral, which were put into each urease test hole. The results were diagnosed with H. pylori infection with urease test by reading biopsyspecimen after 1 hour and 24 hours. With 83.5% patient having used drugs before endoscopy, the percentage of H. pylori (+) from the corpus and the antral specimen is significantly different (65% vs 68.5%) but using specimens from both sites increases significantly the H. pylori positivity from 65% to 78%

Variable number tandem repeat polymorphism of MUC5AC was not associated with H.pylori infection

Background: Some researchers indicated that investigating MUC5AC is necessary for H.pylori infection because H.pylori is easy to locate in MUC5AC. However, there is no publication about the effect of variable number tandem repeat polymorphism of MUC5AC on H.pylori infection. Objective: To investigate the relationship between mucin 5AC variable number tandem repeated polymorphism and H.pylori infection. Subject and Method: 170 patients were studied, mean age: 43, male: 35%. Blood samples were collected from patients, who visited Can Tho General Hospital for upper gastrointestinal endoscopy. DNA was isolated from whole blood; the repeated section of the MUC5AC alleles was not cut out with a restriction enzyme (SacI). H.pylori infection was diagnosed with 14 C urea breath test. Distributions of allele fragment length were compared to H.pylori positive and negative patients. Results: 52% of patients were H.pylori infected. MUC5AC SacI-restricted allele fragment lengths ranged from 6.2 to 11.2 kbp (mean 7.9), the calculated number of repeated ranged from 170 to 380 (mean 243). Mean allele length was identical for H.pylori positive and negative patients were 7.9 kbp, and the distribution of allele fragment lengths was also similar for both groups (p=0.7). Conclusion: The number of repeats in the MUC5AC gen was highly polymorphic, but the variation in allele length was limited. The variable number tandem repeat polymorphism of MUC5AC was not associated with H.pylori infection.