Subcellular location of HCAP1 by its expression and preparation of antiserum / 中国免疫学杂志

Objective:To obtain anti HCAP1 polyclonal antiserum and define subcellular localization of HCC associated protein(HCAP1).Methods:The HCAP1 was expressed in E.coli DE3 cells and purified.The antiserum was prepared by immunizing rabbit with purified HCAP1 protein.The expression of HCAP1 was analyzed by Western blot in the HCC cells lines.The subcellular localization was observed using fluoroimmunocytochemistry.Results:The rabbit antiserum was obtained and HCAP1 was localized in cytoplasm and nucleoli.Conclusion:The expressed HCAP1 and anti HCAP1 antiserum could be used for studying the HCAP1 role in the HCC;The expression pattern of HCAP1 was mainly in cytoplasm and it was also detected in the nucleoli.