ABSTRACT
Objective: To establish the evaluation of the chemial quality standard of Radix Astragali according to their contents of saponin, flavonoid and polyscaccharide. Methods: HPLC ELS was used to determine astragaloside Ⅳ content. RP HPLC was used to determine 10 hydroxy 3,9 dimethoxypterocarpan content. Sulfuric acid phenol method was used to determine total polysaccharide content. Results: Astragaloside Ⅳ, total flavonoid, 10 hydroxy 3,9 dimethoxypterocarpan and total polysaccharide content of Astragalus mongmolicus Fisch. were 0.710? 0.030mg/g, 45?4.243mg/g, 19.955?0.813?g/g, 73.310?0.962mg/g, respectively; Astragaloside Ⅳ, total flavonoid, 10 hydroxy 3,9 dimethoxypeterocarpan and total polysaccharide content of Astragalus membranacens Fisch. were 1.186?0.024mg/g, 46?1.414mg/g,84.640?3.069?g/g, 48.800?1.245mg/g, respectively. Conclusion: The method was practical and provided a new idea to study the chemical quality standard of traditional Chinese drug.