ABSTRACT
Objective:To analyze the chemical constituents in Radix astragali by high-performance-liquid chromatography-time of flight mass spectrometry(HPLC-TOF/MS). Methods:An Agilent poroshell 120 SB-C18 column(100 mm × 3 mm,2. 7 μm)was adopt-ed. The mobile phase was composed of acetonitrile-0. 1% formic acid with nonlinear gradient elution. The flow rate was 0. 4 ml· min-1 . The UV detection wavelength was set at 254nm, the column temperature was 25℃ and the injection volume was 10μl. Electron spray ionization and positive mode was adopted, the flow and temperature of the carrier gas( N2 ) was 10 L·min-1 and 350℃, respec-tively. The capillary voltage was 4 kV, the bombardment voltage was 165 V, the spectra were recorded within the range of m/z 100~1 100. Results:A total of 24 chemical constituents were identified from Radix astragali by HPLC-TOF/MS simultaneously. Conclu-sion:An efficient and fast HPLC-TOF/MS approach has been established for studying the chemical constituents in Radix astragali, which lays the foundation for the study on pharmacodynamic material basis and quality control of Radix astragali.
ABSTRACT
Objective: To identify the main active compounds in the aqueous extract from the aerial parts of Lycopus lucidus by high performance liquid chromatography (HPLC)-electrospray (ESI)-time of flight tandem mass spectrometry (TOF-MS/MS). Methods: The aqueous extract from the aerial parts of L. lucidus was prepared using ultrasonic method; Chromatographic separation of the main active compounds was performed on a TC-C18 (250 mm × 4.6 mm, 5 μm) reverse phase column through gradient elution; All the compounds eluted from the column were detected under both positive and negative ionization modes. Each chromatographic peak was analyzed by quadrupole tandem mass spectrometry coupled with TOF. Results: Twenty two compounds were identified through the analysis of tandem mass spectrum and the information from reference substances, including amino acids, phenolic acids, terpenoids, flavonoids and flavonoid glycosides, sterols, and fatty acid. Conclusion: HPLC-ESI-TOF-MS/MS is capable of analyzing the main compounds in the aerial parts of L. lucidus using retention time, ultraviolet spectrum, current molecular weight, formula, and fragmenting information of daughter ions. It will probably become a reliable alternative for the rapid analyzing substantial foundation of Chinese materia medica.