Huge keratocystic odontogenic tumour in medically compromised patient–a management dilemma

Keratocystic odontogenic tumour (KCOT) is a cystic lesion of the jaws with tumourbehaviour. Its high prevalence rate makes it one of the commonest cystic lesions especially involving the lower jaw. The characteristic histologic features and aggressive nature corresponds to the high recurrence rate associated with KCOT. Lesion expands mostly in an anteroposterior direction and can cause extensive bone destruction before the appearance of any clinical symptoms. The characteristic radiological picture is that of a multilocular cystic lesion with the common differential diagnosis being dentigerous cyst and ameloblastoma. Here we are presenting a case of KCOT of the left lower jaw of size 10.9×7.86×8.54 cm. It is a huge multilocular cystic lesion extending from the right canine region to the left side involving the body, ramus, coronoid and condyle. Various management options are there ranging from enucleation and chemical cauterization to resection and reconstruction depending upon the size of the lesion. In this case we were not able to perform the ideal treatment option for the case because of the multiple drug allergy the patient was having, including most of the general anesthetic agents. Also the patient was not willing for any extensive procedure under general anesthesia. So we had to follow a compromised treatment plan aiming to reducethe size of the lesion, to improve the aesthetics and frequent follow up

Spo0A positively regulates epr expression by negating the repressive effect of co-repressors, SinR and ScoC, in Bacillus subtilis.

Bacillus subtilis under nutritional deprivation exhibits several physiological responses such as synthesis of degradative enzymes, motility, competence, sporulation, etc. At the onset of post-exponential phase the global response regulator, Spo0A, directly or indirectly activates the expression of genes involved in the above processes. These genes are repressed during the exponential phase by a group of proteins called transition state regulators, e.g. AbrB, ScoC and SinR. One such post-exponentially expressed gene is epr, which encodes a minor extracellular serine protease and is involved in the swarming motility of B. subtilis. Deletion studies of the upstream region of epr promoter revealed that epr is co-repressed by transition state regulators, SinR and ScoC. Our study shows that Spo0A positively regulates epr expression by nullifying the repressive effect of co-repressors, SinR and ScoC. We demonstrate via in vitro mobility shift assays that Spo0A binds to the upstream region of epr promoter and in turn occludes the binding site of one of the co-repressor, SinR. This explains the mechanism behind the positive regulatory effect of Spo0A on epr expression.

Impact of 4HPR on the Expression of E-Cad in Human Bladder Transitional Epithelial Cancer Cells T24 / 华中科技大学学报（医学）（英德文版）

Previous researches showed that the expression level of E-Cad in most infiltrating cancer cells was reduced or negative.This study explored whether 4HPR restrained the infiltration of bladder cancer cells through regulating the expression of E-Cad.The infiltrating bladder cancer cells T24 were cultured,and then treated by a proper dosage of drug.Their viability was a determined by MTT method.Western blotting and RT-PCR were adopted to detect the changes of E-Cad gene expression at both protein and mRNA levels.Moreover,immunofluorescent staining and confocal fluorescence microscopy were employed for the observation of the expression of E-Cad.The result showed that,at both mRNA and protein levels,the expression level of E-Cad in T24 cells treated by 4HPR was significantly higher than that of control group,while the β-Cat expression was also relocated from the cell nucleus to cytoplasm.Our findings suggested that the regulatory function of 4HPR on infiltration of bladder cancer cells T24 is at least partly achieved by regulating the expression of E-Cad.

Inhibitory effect of 4-HPR combined with DDP on xenografted ovarian carcinoma in nude mice and its relationship with VEGF and u-PA expression / 肿瘤

Objective: To explore the inhibitory effect of N4-hydroxyphenyl retinode (4-HPR) used alone or in combination with DDP on xenografted human ovarian carcinoma in nude mice and its relationship with the expression of vascular endothelial growth factor (VEGF) and urokinase type plasminogen activator (u-PA). Methods: Human SKOV3 ovarian carcinoma cells were inoculated into BALB/c nude mice. The tumor-bearing nude mice were randomly divided into four groups: control group, DDP group, 4-HPR group, and 4-HPR plus DDP group. The growth of xenografted tumors was observed every 3 days. The VEGF expression in xenograft tumors was analyzed by immunohistochemical staining. The mRNA and protein expressions of VEGF and u-PA were analyzed by RT-PCR and Western blotting, respectively. Results: 4-HPR combined with DDP significantly inhibited the growth of xenografted ovarian carcinoma and down-regulated the mRNA and protein expressions of VEGF and u-PA. The inhibitory effect of combination therapy was superior to those treated with 4-HPR and DDP alone. Conclusion: 4-HPR plus DDP significantly inhibited the proliferation of xenografted ovarian carcinoma in nude mice. The effect was associated with the expressions of VEGF and u-PA.

RECIPROCAL PROJECTION BETWEEN THE HYFOTHALAMUS AND THE SPINAL CORD INNERVATING PELVIC ORGANS OF THE RABBIT——HRP ANTEROGRADE AND RETROGRADE METHODS / 解剖学报

46 rabbits were divided into two groups. HRP was injected into one of the 7 segments of spinal cord (T_(11,12)、L_(1,2)、S(2～4)) unilaterally in 42 adult rabbits in the experimental group. HRP was also injected into the vein of the ear (2 cases) and cerebrospinal fluid (2 cases) as the control group. The hypothalamus were cut at 40 ?m. The sections were incubated with BDHC and OD by Mesulam's 8th and Edward's procedures. Labeled cell were found in the medial basal hypothalamus (MBH), ventromedial nucleus (VM), paraventricular nucleus (PV), dorsomedial nucleus (DM), posterior nucleus (PN), supraoptic nucleus (SN), lateral nucleus (LN), anterior nucleus (AN), perifornical nucleus and zona incerta in all cases. The PV was most heavily labeled. The LN, PN and DM were less so. Some labeled cells were found in the MBH, VM and AN. A few labeled cells were present in the SN. Labeled terminal and preterminal axons were found in the dorsolateral margin of the PV and in the LN、PN and MBH.Labeled terminals of the hypothalamus, which were discovered in this study, has not mentioned in the literature available to us. This study demonstrated reciprocal projection between the hypothalamus and the segments of the spinal cord innervating pelvic organs.