ABSTRACT
Rakta Dhatu is the main source for life longevity and survival of human beings. Formation of Rakta Dhatu is a continuous process. The heterologous food after the action of bio-energies (Agni) in digestion phase is converting into homologous form. Agni-bala (strength of bio-energies) are main helping hand for 2nd Dhatu i.e. Rakta Dhatu (blood cells) in the metabolic action of the body. Further the bio-energies works with changing need of the essence part as absorption and metabolism are started so that generation of tissues (Dhatus) occurs smoothly in the body. The Dhatu Siddhanta (tissue forming laws) in which theories of tissue formation and tissue differentiation were mentioned. The Ahara Rasa (Productive essence part) with the help of different bio-energies in different areas in body helps to produce tissues (Dhatus). To understand how the tissues are formed it is necessary here to understand the logics of Ayurvedic concepts or Siddhanta that proves the formation of Dhatu from Ahara Rasa. The modern science explains the formation of blood cells in embryonic stage by the help mesenchymal cells. The applied aspect of this work is to prevent disorders before their manifestations occurs in the human body. Various diseases that occur due to many causative agents are like- food/Ahara or Agni-Bala (strength of bio-energies), getting poor food digestion or metabolism. On the Ahara ground many different type of etio-pathogenis are aroused due to malfunctioned food or due to malabsorption (Dhatuagnivikriti) that may leads to Dhatuagninasana (metabolic dysfunction). To understand deeply we have to go step by step the physiological ground of food digestion, its laws and how the blood tissues are formed from the sciences, applied aspects of Dhatu Nirmana and its vitiated factors.
ABSTRACT
Objective To study the radioprotective potential of CBLB502 protein against hemopoiesis injury by irradiat-ion. Methods C57BL/6J mice were assigned to normal irradiation, and CBLB502 0.2mg/kg 30min pre-irradiation group at random before 6.5 Gy 60Co-γray total body irradiation (TBI). The peripheral blood was obtained to assay hemogram pre and post-irradiation. The bone marrow nucleated cell counts were evaluated in mice at 2 h and 24 h after irradiation. Colony-forming unit (CFU) assay was used to analyze the alteration of hemopoietic progenitor cells in bone marrow. Competitive repopulation assay was conducted to observe the implantation ability of bone marrow cells. The percentage of each lineage hemopoietic cells was measured by flow cytometry analysis. Results CBLB502 significantly alleviated the sharp decrease of peripheral blood counts including leukocyte (WBC), erythrocyte (RBC), and platelet, and accelerated recovery. Counts of various hematopoietic progenitor cell colonies of mouse bone marrow in CBLB502 group were significant higher than those of irradiation group (P<0.01). CBLB502 significantly improved the implantation ability of bone marrow cells. Conclusion CBLB502 showed obvious radioprotective effects on haemopoiesis injury by irradiation.
ABSTRACT
Objective To study the radioprotective potential of CBLB502 protein against hemopoiesis injury by irradiation. Methods C57BL/6J mice were assigned to normal irradiation, and CBLB502 0.2mg/kg 30min pre-irradiation group at random before 6.5 Gy 60Co-! ray total body irradiation (TBI). The peripheral blood was obtained to assay hemogram pre and post-irradiation. The bone marrow nucleated cell counts were evaluated in mice at 2 h and 24 h after irradiation. Colony-forming unit (CFU) assay was used to analyze the alteration of hemopoietic progenitor cells in bone marrow. Competitive repopulation assay was conducted to observe the implantation ability of bone marrow cells. The percentage of each lineage hemopoietic cells was measured by flow cytometry analysis.
ABSTRACT
Involvement of pineal and its major hormone, melatonin, in the process of erythropoiesis in a freshwater catfish, Clarias batrachus has been investigated. The study was conducted during four phases, namely preparatory phase, spawning phase, postspawning phase and late postspawning phase of its annual reproductive cycle. During each phase a fish received either melatonin injections or subjected to pinealectomy. In addition, each fish in all the groups, received either iopanoic acid or cyproterone acetate or vehicle in the morning or late afternoon. Results clearly indicate that melatonin stimulates the rate of erythropoiesis in Clarias batrachus. It appears that the extent of stimulation depends upon the phase of the annual reproductive cycle. However, in general, the pineal- or melatonin-induced modulation of blood variables is gonad dependent and thyroid seems to play a time of the day dependent subtle role.
ABSTRACT
40 human fetuses were used in this study. The range of fetal age varied from 3 months to full terms. All fetuses used were normal and their parents were healthy. The CFU-C from bone marrow, liver, spleen and peripheral blood were studied in vitro with agar culture technique modified by Metcalf.Experiments proved that the CFU-C of bone marrow may be observed in the fetuses in 3rd month, however, it increased rapidly in 4th month and maintained higher level until the end of fetal life. The progenitor cell population was detected at very high level in liver from 4th month to 6th month fetuses. The number of CFU-C of fetal liver were low in 7—10th month. The circulating progenitor cells in peripheral blood of 4, 5 and 6-month fetuses Were on high level but it was lower than that of liver and bone marrow.