ABSTRACT
Haemorrhagic Septicaemia is one of the most common, fatal and acute bacterial diseases of livestock which causes mortality above 70% and is caused by Pasteurella multocida. The only satisfactory and practical method of control and prevention is timely vaccination of all the healthy and in contact animals. Different types of vaccines are being used for the immunity against this disease. In this project three oil based vaccines were produced. Two single emulsion vaccines were prepared by utilizing Montanide ISA-50 and liquid paraffin with lanolin where as one double emulsion with the help of Montanide ISA-206 was prepared. In house quality control testing and safety testing was performed on swiss albino mice. For immune titre IHA was performed by collecting serum from each and every animal including control animals. The comparison of IHA was done via statistical analysis by using GMT, Single emulsion vaccine prepared from liquid paraffin with lanolin gave maximum immune titre out of all the three vaccines in large animals and in young calves ISA 206 gave a significant titre.
ABSTRACT
Haemorrhagic Septicaemia (HS), an acute and fatal disease of cattle and buffalo is primarily caused by serotype B:2 or E:2 of Pasteurella multocida. The transferrin binding protein A (TbpA) has been found to act as immunogen and potent vaccine candidate in various Gram negative bacteria including P. multocida. The present study was carried out to evaluate the potential of this antigen as a DNA vaccine against HS in mice model. The tbpA gene of P. multocida serotype B:2 was cloned in a mammalian expression vector alone and along with murine IL2 gene as immunological adjuvant to produce monocistronic and bicistronic DNA vaccine constructs, respectively. The immune response to DNA vaccines was evaluated based on serum antibody titres and lymphocyte proliferation assay. A significant increase in humoral and cell mediated immune responses was observed in mice vaccinated with DNA vaccines as compared to non immunized group. Additionally, the bicistronic DNA vaccine provided superior immune response and protection level following challenge as compared to monocistronic construct. The study revealed that DNA vaccine presents a promising approach for the prevention of HS.