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Objective: To assess the infection status of Cryptosporidium spp. in the four common species of farmed animal (pigs, cattle, goats and geese) in the Hainan Island of China, followed by molecular characterization of the parasite. Methods: During March to July 2019, 864 DNA were extracted from the fecal samples of 188 pigs, 341 goats, 130 cattle, and 169 geese which were collectd from four, five, six, and three cities of the Hainan Province, China, respectively. The species/genotypes and subtypes of Cryptosporidium were identified by employing nested-PCR amplification of the SSU rRNA and the 60 kDa glycoprotein (gp60) genes of the parasite, respectively. Results: Cryptosporidium was identified in 68 (7.9%) out of 864 animals, with 45 (13.2%) goats, 13 (6.9%) pigs, 4 (3.1%) cattle, and 6 (3.6%) geese. Cryptosporidium (C.) xiaoi (n=24) and C. ubiqutium (n=21) were identified in goats; C. suis (n=7) and C. scrofarum (n=6) in pigs; C. parvum (n=3) and C. occultus (n=1) in cattle; and C. baileyi (n=1) and Cryptosporidium goose genotype I(n=3) in geese. The 21 C. ubiqutium isolates shared a same subtype XII a, and the subtype of three C. parvum was II dA15G1. Conclusions: We present for the prevalence of the Cryptosporidium in cattle, goats, pigs and geese from the Hainan Island of China for the first time. The molecular characterization of the Cryptosporidium isolates obtained here propose that the goats, cattle and pigs are potential sources of C. ubiqutium, C. parvum, and C. suis infections, in humans.
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Objective: To analyze the distribution of hepatitis B virus (HBV) genotypes in Hainan island, which is one of the most endemic areas of HBV infection in China. Methods: A total of 52 patients with chronic HBV infection were enrolled. HBV genotypes were determined by nested polymerase chain reaction using genotype-specific primer pairs. Results: All patients were positive for nested polymerase chain reaction and the genotype B was detected in all patient serum samples. Moreover, 23 of 52 patients (44.2%) have been detected with genotype B and C coinfection. Conclusions: HBV genotype B is mostly prevalent in Hainan island. Intriguingly, co-infection of genotype B and C was frequently detected in Hainan island, but it may need to be further verified by HBV full genome sequencing analysis.
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Objective To investigate the allele polymorphism and characteristic of HPA-1-17 genotyping of Li ethnic on Hainan island,and to evaluate the HPA mismatched ratio between donor and recipient in randomly chosen cases of blood transfusion,in order to investigate the platelet transfusion refractoriness in Li population.Methods Genotyping tests of A-1—17 allele of the blood sample from 180 Lis were conducted with PCR-SSP.Results A total of 180 blood samples were genotyped successfully in HPA genetic system,among which 0.9972 were HPA-2a,0.0028 were HPA-2b,0.4889 were HPA-3a,0.5111 were HPA-3b,0.9667 were HPA-5a,0.0333were HPA-5b,0.9972 were HPA-6a,00028 were HPA-6b,0.4250 were HPA-15a,0.05750 were HPA-15b,and they all showed polymorphism. But polymorphism was not detected out in other allelic genes,such as HPA-1,-4,-7—14,-16,and -17. The bb homozygous were detected in two alleles,among which 0.2834 was HPA-3 and 0.3667 was HPA-15,but it wasn't detected in the others systems. In the randomly chosen cases of blood transfusion,the mismatch rate of HPA-3 ,HPA-15 and HPA-5 was 37.49%,36.93% and 6.23%,respectively.Conclusion This research revealed the distribution of HPA gene in Li population on Hainan island,and found the allelic frequency and thier characteristics. According to our findings,HPA-2,HPA-3,HPA-5 and HPA-15 genes between donor and recipient should be matched in order to avoid platelet transfusion refractoriness.