ABSTRACT
OBJECTIVE@#To analyze the dynamic expression of Wnt family member 5A (Wingless-type MMTV integration Wnt site family, member 5a) in murine hair cycle and its inhibitory effects on follicle in vivo.@*METHODS@#Situ hybridization in full-thickness skin was used to observe the change of mouse protein expression in different growth stages, and Ad-Wnt5a was injected after defeathering to observe the hair follicle growth in vivo.@*RESULTS@#The Wnt5a mRNA was expressed at birth, and was firstly increased then decreased along with the progress of the hair cycle. It reached the peak in advanced stage of growth cycle (P<0.05). Rhoa and β-catenin expression levels were significantly decreased in three groups. Rac2 expression was significantly up-regulated, and the expression level of Wnt5a, Shh and Frizzled2 was increased, but less significantly than group 2.@*CONCLUSIONS@#The expression of Wnt5a mRNA is consistent with change of murine follicle cycle, and has obvious inhibitory effects on the growth of hair follicle in vivo, indicating that it is antagonistic to Wnts pathway and interferes the growth of follicle together.
Subject(s)
Animals , Mice , Chi-Square Distribution , Gene Expression Profiling , Hair Follicle , Chemistry , Metabolism , RNA, Messenger , Genetics , Metabolism , Real-Time Polymerase Chain Reaction , Skin , Metabolism , Wnt Proteins , Genetics , Metabolism , Wnt-5a ProteinABSTRACT
Objective To investigate the expression and significance of androgen receptor(AR) of rat vibrissa follicle stem cells in culture and vibrissa follicle stem cells in vivo at three different phases of a hair follicle cycle,including anagen,catagen and telogen.Methods AR was detected using immunohistochemistry.The expression of AR mRNA was examined by total RNA extraction and RT-PCR.Results In vivo the vibrissa follicle stem cells expressed AR in the whole hair follicle cycle.AR mRNA was strongly expressed in anagen,weakly in catagen and telogen.AR expressed in the cultured vibrissa follicle stem cells.Conclusion The expression of AR mRNA was altered regularly in rat vibrissa follicle stem cells in vivo throughout the hair follicle cycle,which suggested that the interaction between androgen and AR may facilitate the differentiation of vibrissa follicle stem cells.