ABSTRACT
Objective: To optimize the extracting methods and technology for total alkaloids from the seeds of Crinum asiaticum. Methods: Ultrasonic extraction and reflux extraction were used to extract the total alkaloids from the seeds of C. asiaticum. Results: The two results showed that the extracting rate of alkaloids by ultrasonic extraction was 0.4357% under ultrasonic temperature of 58°C, ultrasonic time of 47 min, ultrasonic power of 600 W, percentage of ethanol volume of 95%, solid-liquid ratio of 1:6, and extraction for three times. Additionally, the extracting yield of alkaloids by reflux extraction was 0.4494% under extraction temperature of 84°C, extraction time of 2.5 h, solid-liquid ratio of 1:9, percentage of ethanol volume of 95%, and extraction for three times. Conclusion: Considering the extracting rate of alkaloids, operating methods, and the difficulty of removing impurity, the heating reflux extraction is an ideal method for total alkaloids extracting from the seeds of Crinum asiaticum.
ABSTRACT
OBJECTIVE: To study extraction method of anthraquinones components of Radix et Rhizoma Rhei and optimize extraction technology.METHODS: Ultrasonic extraction,heating reflux extraction and soxhlet extraction were adopted to extract Radix et Rhizoma Rhei respectively.3 kinds of extraction technology were compared with the content of total anthraquinone,aloe-emodin,rhein,emodin,chrysophanol,physcione as index.The extraction technologies of UV and 5 kinds of anthraquinone compounds were optimized by uniform design method on the basis of previous study.RESULTS: Heating reflux extraction which was optimal extraction technology was as follows: extracting time of 90 min,extracting for 1 time,methanol concentrations of 95%,medicinal mesh of 2.000 7.Comprehensive score of anthraquinones could reach 6.556.CONCLUSION: The extraction technology is reasonable,available in quality control.