A new compound from roots and rhizomes of Helleborus thibetanus / 中草药

Objective: To study the chemical constituents in the ethanol extract from the roots and rhizomes of Helleborus thibetanus. Methods: The compounds were isolated and purified by silica gel column chromatography and recrystallization. The structures of the compounds were identified on the basis of chemical and spectral methods. Results: A new compound was isolated from the ethanol extract in the roots and rhizomes of H. thibetanus and identified as 14β-hydroxy-3β - [(β-D-glucopyranosyl)oxy]-5α-bufa-20, 22-dienolide (1). Conclusion: Compound 1 is a new compound named as hellebocoside A, which belongs to bufadienolide of cardiac glycosides.