ABSTRACT
Objective To study the influence of total flavonoids of hemerocallis fulva(TFHF) on hepatocyte apoptosis and related protein expression in mice with alcoholic hepatic injury.Methods A total of 40 mice were randomly divided into four groups:blank control,model control andsmall and high dose TFHF groups,10 cases in each group.The mice were given the continuous gavage administration for 7 d.Then the model group was given once gavage by 50％ ethanol 12.0 mL/kg after 1 h of the last administration.The blank control group was given the equal volume of distilled water.The activity levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum as well as the superoxide dismutase(SOD) activity and malondialdehyde (MDA) content in liver tissue hemogenate were detected.Hematoxylin and Eosin(HE) staining was performed for observing the pathological changes of the liver tissue.The flow cytometer was used to test the apoptosis ratio in hepatocyte suspension.The expressions of caspase-3,Bcl-2 and Bax protein were detected by Western blot.Results The various TFHF groups could decrease the activities of ALT and AST in serum (P＜0.05),while could decrease the MDA content in liver tissue hemogenate (P＜0.01) and increased the SOD activity;the liver tissue pathological examination showed that the high dose TFHF group could make the liver cell degeneration,alleviated the necrosis degree and relieved the pathological change of hepatic tissue;compared with the model group,the hepatocyte apoptosis rate in each TFHF group was decreased significantly;Western blotting results showed that the caspase-3 protein level in each TFHF group was decreased,expression of Bcl-2 protein was increased,whereas which of Bax protein was decreased and Bax/Bcl-2 ratio was reduced.Conclnsion TFHF has obvious protective effect on mice acute hepatic injury induced by ethanol,and can inhibit the hepatocyte apptosis,its action mechanism may be related to its antioxidation and regulation of caspase-3,Bcl-2 and Bax expression.
ABSTRACT
Objective: To revise the structure of hemerocallin, an active and toxic compound isolated from the roots of Hemerocallis fulva, which was first reported in 1974 based on the chemical and spectral data. Methods: The structure of acetyl hemerocallin was determined through the acetyl derivative preparing and X-ray diffraction. Results: The title compound acetyl hemerocallin (1) has been prepared and structurally characterized by X-ray single-crystal diffraction method. X-ray analysis revealed that the two naphthalene rings in compound 1 were significantly twisted with an average dihedral angle of 44.1°. The two acetate groups in each naphthalene ring adopted anti-parallel conformation. In addition, the π-π stacking interactions were found in the crystal structure, the molecules were linked into a supramolecular network, and the crystal structure was stabilized. Conclusion: The structure of hemerocallin is identified as 2,2'-bi(1,8-dihydroxy-6-methyl-7-acetylnaphthalene) (3). © 2013 Tianjin Press of Chinese Herbal Medicines.
ABSTRACT
In July 2002, a destructive stem rot of tawny daylily(Hemerocallis fulva) was occurred sporadically in exhibition farm of Gyeongsangnam-do Agricultural Research and Extension Services located in Hamyang-gun, Korea. The fungus also caused collar and crown rot, and systemic wilt or blight of whole plant. White mycelium spread over stems and petioles of infected plants and sclerotia were formed on the old lesions and near the soil surface. The optimum temperature for mycelial growth and scierotial formations was 30degrees C on PDA. The mycelial width ranged 4.2~10.4microm and the color was white, usually many narrow mycelial strand grew in the aerial mycelium and formed clamp connection. The shape of sclerotia was spherical and 1.0~3.2 mm in diameter. The fungus was isolated repeatedly from the infected tissues and confirmed its pathogenicity to Hemerocallis fulva and identified as Sclerotium rolfsii. This is the first report on the stem rot of H. fulva caused by S. rolfsii in Korea.
Subject(s)
Crowns , Fungi , Hemerocallis , Korea , Mycelium , Plants , Soil , VirulenceABSTRACT
Objective To study the chemical constituents in the roots of Hemerocallis fulva. MethodsCompounds were isolated from the CHCl3and n-butanol fraction of the EtOH extract by repeated column chromatography and their structures were identified by spectral analysis and physicochemical properties. Results From the CHCl3 and n-butanol fraction of the EtOH extract in the roots of H. fulva,ten compounds were isolated and identified as terresoxazine (Ⅰ),11-keto-?-boswellic acid (Ⅱ),hopane-6?,22-diol (Ⅲ),hecogenin (Ⅳ),stigmast-4-en-3?-ol (Ⅴ),stigmast-4-en-3-one (Ⅵ),?-feruloyloxy acid (Ⅶ),(E)-3,4-dihydroxyl cinnamic acid (Ⅷ),(E)-p-methyl cinnamic acid (Ⅸ),and vanillic acid (Ⅹ). Conclusion Compounds Ⅰ-Ⅳ,and Ⅶ are isolated and identified from the plants of Hemerocallis L. for the first time.