ABSTRACT
Background: In this work, the xylanase production by Penicillium chrysogenum F-15 strain was investigated using agroindustrial biomass as substrate. The xylanase was purified, characterized and applied in hemicellulose hydrolysis. Results: The highest xylanase production was obtained when cultivation was carried out with sugar cane bagasse as carbon source, at pH 6.0 and 20°C, under static condition for 8 d. The enzyme was purified by a sequence of ion exchange and size exclusion chromatography, presenting final specific activity of 834.2 U·mg·prot-1. T he molecular mass of the purified enzyme estimated by SDS-PAGE was 22.1 kDa. The optimum activity was at pH 6.5 and 45°C. The enzyme was stable at 40°C with half-life of 35 min, and in the pH range from 4.5 to 10.0. The activity was increased in the presence of Mg+2 and Mn+2 and reducing agents such as DTT and ßmercaptoethanol, but it was reduced by Cu+2 and Pb+2 . The xylanase presented Km of 2.3 mM and Vmax of 731.8 U·mg·prot-1 with birchwood xylan as substrate. This xylanase presented differences in its properties when it was compared to the xylanases from other P. chrysogenum strains. Conclusion: The xylanase from P. chrysogenum F-15 showed lower enzymatic activity on commercial xylan than on hemicellulose from agroindustry biomass and its biochemistry characteristics, such as stability at 40°C and pH from 4.0 to 10.0, shows the potential of this enzyme for application in food, feed, pulp and paper industries and for bioethanol production.
Subject(s)
Penicillium chrysogenum/metabolism , Polysaccharides/metabolism , Endo-1,4-beta Xylanases/biosynthesis , Temperature , Enzyme Stability , Biomass , Endo-1,4-beta Xylanases/isolation & purification , Electrophoresis, Polyacrylamide Gel , Hydrogen-Ion Concentration , HydrolysisABSTRACT
O objetivo deste estudo foi avaliar as propriedades de polpas kraft marrons de madeiras de
The objective of this study was to evaluate properties of brown pulp from
ABSTRACT
Xylanases are in the focus of research due to their potential to replace many current polluting chemical technologies by biochemical conversion. The field of application for xylanases is vast; it comprises industrial applications like wood pulp bio-bleaching, papermaking and bioethanol production. In addition, these enzymes can be applied as additives in food and beverage industry, and animal nutrition. However, considering the potential applications for these enzymes, the market share of xylanases is still low. Thus, the search for promising xylanases which tolerate relevant processing conditions and therefore can be used in industrial settings is an ongoing task. This review provides an overview of the enzymes reported from 2012 to mid 2014. Further, legal restrictions for the use of (genetically modified) organisms and enzymes are considered. This review provides an integrated perspective on the potential of specific xylanases for industrial applications.