ABSTRACT
Background: Forkhead box A2 (Foxa2) plays an important role in the proliferation and distant metastasis of colorectal cancer, but the expression of Foxa2 in colorectal polyps has not been reported yet. Aims: To investigate the expression and significance of Foxa2 in colorectal polyps and colorectal cancer. Methods: A total of 45 patients with hyperplastic polyps, 135 patients with adenomatous polyps and 45 patients with colorectal cancer and corresponding adjacent tissue from January 2018 to May 2019 at Renmin Hospital of Wuhan University were collected. Immunohistochemical SP staining was used to detect the expression of Foxa2. Expressions of Foxa2 mRNA and protein were determined by real-time fluorescence quantitative PCR and Western blotting, respectively. The relationships between the expression of Foxa2 and colorectal polyps, colorectal cancer were analyzed. Results: The positive expression rate of Foxa2 in normal colorectal mucosal tissue, hyperplastic polyps, adenomatous polyps and colorectal cancer was gradually increased (13.3%, 31.1%, 62.2%, 86.7%, respectively), and the difference was statistically significant (P<0.05). The expressions of Foxa2 mRNA and protein were also increased, and the differences were statistically significant (P<0.05). The expression of Foxa2 in adenomatous polyps was related to size of polyps and presence of pedicle (P<0.05); the expression of Foxa2 in colorectal cancer was related to differentiation, lymph node metastasis and TNM staging (P<0.05). Conclusions: The expression of Foxa2 is high in adenomatous polyps and colorectal cancer, and increases with the increase of risk of canceration. Therefore, detecting the expression of Foxa2 in colorectal polyps is helpful for the early detection of colorectal cancer.
ABSTRACT
Objective To investigate the expression and clinical significance of FoxA 2(hepatocyte nuclear factor 3β)in human non-small cell lung cancer(NSCLC)tissues and adjacent normal lung tissue.Methods The expressions of FoxA 2 protein in 80 cases with NSCLC tissues and adjacent normal lung tissue were tested by immunohistochemistry,the expressions of mRNA was detected by RT-PCR.Results The expressions of FoxA 2 both on protein and mRNA in NSCLC lung tissue were significantly less than those of adjacent normal lung tissue(protein expression comparison t value was 187.51,186.43,178.24 respectively;mRNA expression comparison t value was 236.70,260.13,126.27,respectively,P ＜ 0.05).However there was no difference on histopathological variability.F value equal to 1.86 and 1.69,P ＞ 0.05).In low differentiated group,the group of Ⅲ stage,with lymph node metastasis,the expression of FoxA 2 protein and mRNA was less than that in the group of high differentiated,the group of Ⅰ-Ⅱ stage,the group of non lymph node metastasis(pretein expression t values were 4.31,3.92,341,mRNA expression t values were 5.44,3.28,5.07,P＜0.05).Conclusion FoxA 2 might inhibit progression of human NSCLC.It may participate in the process of tumor tissue differentiation and lymph node metastasis in NSCLC.