ABSTRACT
This study was carried out to investigate the in vitro antimicrobial properties of crude methanolic extract ofChromolaena odorata and its interactions with some standard antibiotics (ofloxacin, ciprofloxacin, and gentamicin)on Pseudomonas aeruginosa isolated from wound samples. P. aeruginosa was isolated from wound samplesfrom hospital patients in Enugu State, Nigeria, using standard bacteriological methods. Methanolic extraction ofC. odorata was carried out using Soxhlet extractor. The antimicrobial activity and in vitro interactions were evaluatedusing a combination of agar well diffusion and broth dilution techniques. The findings of this study showed that allthe P. aeruginosa isolates were susceptible to the C. odorata methanolic crude extract at high concentrations. Therewas an enhancement of the potency of the methanolic crude extract when combined with low concentrations ofstandard antibiotics compared to its potency when tested alone. Our findings give credence to the folkloric use ofC. odorata for the treatment of wounds, especially P. aeruginosa-infected wounds. There could be beneficial clinicalapplication of the coadministration of standard antibiotics and the crude extract of C. odorata in the treatment ofwound infections caused by P. aeruginosa.
ABSTRACT
The herb-drug combination is becoming more and more common. The safety evaluation method is a complex evaluation system. This paper analyzed Pubmed database from 2008 to 2018, and summarized the characteristics of safety evaluation method, and the characteristics are listed as below: (1) Exposure safety issues in real world; (2) The combination use is complex and difficult to evaluate. There are the existing safety assessment methods including literature induction, prospective test, questionnaire survey, etc. Although safety evaluation has been carried out from different angles, there is still a lack of comprehensive and reasonable safety evaluation method. The team tried to combine clinical phenotype and mechanism characteristics to provide more reasonable methodological support for clinical safety evaluation.