ABSTRACT
Aim To study the neuroprotective effects of Herba siegesbeckiae extract on cerebral ischemia/ reperfusion rats and its mechanism. Methods Sixty SD rats were randomly divided into model group, low, middle and high dose groups of Herba siegesbeckiae, and Sham operation group, and the drug was given continuously for seven days. The degree of neurologic impairment was evaluated by mNSS, and the infarct volume was measured by MRI. The number of Nissl-posi- tive cells was detected by Nissl staining, and the apop- tosis was accessed by Tunel staining. Furthermore, the expression of Bax, Bcl-2 and NeuN was observed by Western blot, and the expression of NeuN was detected by immunofluorescence staining. The expression of IL- 1β, TNF-α and IL-6 mRNA was performed by RT- qPCR. Results The mNSS score and the volume of ischemic cerebral infarction in the model group were significantly increased, and Herba siegesbeckiae extract treatment significantly decreased the mNSS score and infarct volume (P<0.05, P<0.01). Herba siegesbeckiae extract could increase the number of Nissl-pos- itive cells and the expression of NeuN (P<0.01), and reduce the number of Tunel-positive cells (P<0.01). Western blot showed that Herba siegesbeckiae extract inhibited the expression of Bax, increased Bcl-2 and NeuN in ischemic brain tissue (P<0.01). RT-qPCR showed that Herba siegesbeckiae extract inhibited the expression of IL-1 β, TNF-α and IL-6 mRNA in the is-chemic brain tissue (P<0.01). Conclusions Herba siegesbeckiae extract can reduce the cerebral infarction volume, improve the neurological function damage, inhibit the apoptosis of nerve cells and the expression of inflammatory factors and promote the expression of NeuN, there by exerting protective effects on MCAO rats.
ABSTRACT
The study established a LC-MS/MS method for the simultaneous determination of two active diterpenoids:kirenol and ent-16β, 17-dihydroxy-kauran-19-oic acid (DHKA) from Herba Siegesbeckiae in rat plasma using osthole as an internal standard (IS). Plasma sample pretreatment involved a one-step liquid-liquid extraction with ethyl acetate. Chromatographic separation was performed on a Waters Symmetry C18 column (2.1 mm×100 mm, 3.5 μm) with isocratic elution using methanol-5 mmol·L-1 aqueous ammonium acetate (80:20) as mobile phase at a flow rate of 0.2 mL·min-1. The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode under positive and negative electrospray ionization. Quantification was performed using SRM of the transitions m/z 356.4→321.4 for kirenol, m/z 335.3→335.3 for DHKA, and m/z 245.1→188.9 for the IS, respectively. The calibration curves were linear over the range of 50.0-25000 ng·mL-1 for kirenol, 25.0-12500 ng·mL-1 for DHKA. The extraction recoveries of two analytes and IS were more than 85%. The intra- and inter-day precision (relative standard deviation) values were less than 13.9% and accuracy (relative error) was from -10.7% to 10.3% at four quality control levels. The pharmacokinetic parameters of different medication administration teams were analyzed with SPSS statistics 13.0 software. The validated method was successfully applied to a comparative pharmacokinetic study of the two diterpenoids in rat plasma after intragastric administration of kirenol, DHKA and Herba Siegesbeckiae extract. Kirenol appeared to be both absorbed and eliminated fast in vivo, and DHKA absorbed fast but eliminated slowly in vivo. And there were obvious differences between the pharmacokinetic behaviors after oral administration of Herba Siegesbeckiae extract compared with single substances. Compared with the value after oral administration of kirenol, the extract might inhibit the absorption and postpone the elimination of kirenol in rats after administration of the extract. For DHKA, the absorption rate of DHKA increased rapidly after administration of the extract. This work can provide some experimental basis for the clinical use of Herba Siegesbeckiae. The method is simple, rapid and sensitive, which is suitable for pharmacokinetics study of the two diterpenoids from Herba Siegesbeckiae in rats.
ABSTRACT
OBJECTIVE:To study endothelial NO-dependent vasodilation effect of the extracts of Herba Siegesbeckiae. METHODS:SD rat thoracic aorta rings were used to observe contraction of blood vessel, and the effects of the extracts of Herba Siegesbeckiae on the contraction induced by phenylphrine (PE). RESULTS:The extract of Herba Siegesbeckiae could inhibit the contraction of blood vessel induced by PE. After pretreatment of blood vessel by L-NNA, vasodilation effect of the extracts of Herba Siegesbeckiae was significantly attenuated at low concentration condition(P
ABSTRACT
OBJECTIVE:To conduct a textual research on the medicinal parts of Herba Siegesbeckiae for references of scientific and effective clinical use of Herba Siegesbeckiae. METHODS:Textual research was conducted in a chronological order and which was subjected to analysis based on marketing research. RESULTS:In ancient times, the tender stems and leaves of Herba Siegesbeckiae were used as medical herbs, but lots of thick stems are presented in Herba Siegesbeckiae sold on current market, which is not in line with the medication experience of ancients. CONCLUSION:Great importance should be attached to the medicinal parts in Herba Siegesbeckiae.To make sure its medicinal parts, further research remains to be done.
ABSTRACT
OBJECTIVE:To study the reasonable parts used as medicinal herbs in Herba Siegesbeckiae from the point of chemical contents.METHODS:Using the methods of Ch.P 2005 to determine the percentage contents of ethanol-soluble extractives and Kirenol(an effective antirheumatic constituent) in the tender stems and leaves and thick stems of Herba Siegesbeckiae. RESULTS:The contents of the ethanol-soluble extractives and Kirenol in the tender stems and leaves were much higher than in the thick stems.CONCLUSION:The above results suggest that tender stems and leaves of Herba Siegesbeckiae should be used as the medicinal parts.
ABSTRACT
OBJECTIVE:To study the effect of Herba Siegesbeckiae capsule on cerebral blood flow (CBF), cerebral vessel resistance (CVR), blood pressure (BP), electrocardiogram (ECG) and heart rate (HR)in anesthetized dogs. METHODS: The external carotid arteries on one side and the vertebral arteries of dogs were ligated, then the CBF of carotid arteries was measured by electromagnetic flowmeter, and the BP, EGG and HR were measured by polygraph. RESULTS: Herba Siegesbeckiae capusle could significantly increase CBF and decrease CVR; however, it has no effect on BP, ECG and HR. CONCLUSION: Herba Siegesbeckiae capsule can improve cerebral blood circulation through increasing CBF and decreasing CVR.