One new coumarin from seeds of Herpetospermum pedunculosum / 中国中药杂志

This paper aims to investigate the chemical constituents of the seeds of Herpetospermum pedunculosum. One new coumarin and two known lignans were isolated from the ethanolic extract of the seeds of H. pedunculosum with thin layer chromatography(TLC), silica gel column chromatography, Sephedax LH-20 chromatography, Semi-preparative high performance liquid chromatography and recrystallization, etc. Their structures were elucidated as herpetolide H(1), phyllanglaucin B(2), and buddlenol E(3) by analysis of their physicochemical properties and spectral data. Among them, compound 1 was a new compound, and compounds 2 and 3 were isolated from this genus for the first time. In vitro anti-inflammatory activity test showed that herpetolide H had certain NO inhibitory activity for LPS-induced RAW 264.7 cells, with its IC_(50) value of(46.57±3.28) μmol·L~(-1).

Studies on chemical constituents of stems of Herpetospermum pedunculosum / 中国中药杂志

This project is to study chemical compositions from the stems of Herpetospermum pedunculosum. Twenty-two compounds were isolated from the 70% acetone extract of the stems of H. pedunculosum by column chromatography on Sephadex LH-20, semi-preparative HPLC and preparative TLC. Their structures were elucidated by their physicochemical properties and spectroscopic data as N-benzyltyramine(1), α-spinasterol(2),(2S)-1-O-heptatriacontanoyl glycerol(3), 5,7-dihydroxychromanone(4), methyl 2β,3β-dihydroxy-D:C-friedoolean-8-en-29-oate(5), p-hydroxy benzyl alcohol(6), p-hydroxybenzoate(7), p-hydroxy cinnamic acid(8), 1H-indol-3-carboxylic acid(9), rhodiocyanoside B(10), rhodiolgin(11), rhodiosin(12), 9,12,13-trihydroxy-10(E)-octadecenoic acid(13), cylo-(Tyr-Leu)(14), matteflavoside A(15), loliolide(16), 1H-indol-3-carboxaldehyde(17),(+)-dehydrovomifoliol(18), 3-hydroxy-5α,6α-epoxy-β-ionone(19), 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxy-1-propen-1-yl)-2-methoxyphenoxy]-1-propanone(20), 7-en-nonadecanoic acid monoglyceride(21), vanillic acid(22). Compound 1 is a new natural product, while compounds 3-15 were isolated from this plant for the first time.

Effects of total lignans from tibetan medicine herpetospermum pedunculosum seeds on proliferation and apoptosis of rat hepatic stellate cells / 中草药

Objective To observe the possible mechanism of total lignans from Herpetospermum pedunculosum seeds (TLHPS) in fighting against hepatic fibrosis of hepatic stellate cell-T6 (HSC-T6) by studying the effects of TLHPS on inhibiting proliferation and inducing apoptosis of HSC-T6. Methods The cells were divided into totally six groups such as control group, TLHPS groups (10, 20, 30, 40 μg/mL), and colchicine positive control group (0.1 μg/mL). HSC-T6 cells were cultivated for 24, 48, and 72 h by six groups of culture medium containing different drugs. The proliferation inhibitory rate of HSC-T6 cells was detected by CCK8 method at 24, 48, and 72 h respectively. The apoptotic rate and cell cycle were detected by flow cytometry. Protein expression levels of B cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax) and transcription factors-κB (NF-κB) were detected by Western blotting. Results Compared with control group, 24, 48, and 72 h proliferation inhibitory rate of HSC-T6 was obviously elevated in TLHPS 10, 20, 30, 40 μg/mL groups (P 0.05), cell numbers in S phase obviously decreased (P < 0.01). Compared with control group, Bal-2 protein expression obviously decreased in TLHPS 10, 20, 30, 40 μg/mL groups (P < 0.05, 0.01), Bax protein expression obviously increased in TLHPS 40 μg/mL group (P < 0.01) and NF-κB protein expression obviously decreased in TLHPS 20, 30, 40 μg/mL groups (P < 0.01). Conclusion The mechanism of total lignans from Tibetan medicine Herpetospermum fighting against hepatic fibrosis might be associated with inhibiting proliferation and inducing apoptosis of HSC-T6. The inhibition of hepatic stellate cells proliferation and induction of its apoptosis may be related to lowering the expression of Bcl-2 and NF-κB.

Plant Regeneration through Somatic Embryo in Herpetospermum pedunculosum,an Endangered Tibetan Medicinal Herb / 中草药·英文版

Objective An effective reproducible protocol for complete plant regeneration via somatic embryogenesis has beendeveloped for Herpetospermum pedunculosum,an endangered Tibetan medicinal herb.Methods The cotyledonexplants used in this study were excised from seedlings germinated in vitro.Callus was induced from cotyledonexplants on Murashige and Skoog's medium,supplemented with 2,4-dichlorophenoxyacetic acid(2,4-D,0.1-1.0mg/L)alone or in combination with 6-benzylaminopurine(BA,0.5,1.0,and 2.0 mg/L).Results The calli showeddifferentiation of globular embryos after three weeks of incubation on MS medium supplemented with variouscombinations of BA and NAA.Sixty-two percent of the embryogenic calli produced somatic embryos in MS basalmedium supplemented with BA(1.0 mg/L)+NAA(2.0 mg/L).The addition of KN(0.5 mg/L)to MS mediumcontaining both BA and NAA(2.0 mg/L each)significantly increased the frequency of somatic embryogenesis.Themaximum percentage of embryogenic calli formation was 83%,and globular embryos formed and germinatedsuccessfully in this medium.Then,transferring the regenerated plants from this medium to hormone-free MSmedium will further enhanced the development of the plants,and the healthy plantlets are formed successfullywithin four weeks.The plantlets were transferred to soil to acclimatize under greenhouse conditions and 75%survived.Conclusion Somatic embryogenesis protocol as reported here can play a key role in the propagation andconservation of this endangered species.

RP-HPLC fingerprint researches on the Tibetan crude drug Herpetospermum pedunculosum / 中成药

AIM: In order to evaluate and effectively control the quality of the Tibetan crude drug Herpetospermum pedunculosum,RP-HPLC fingerprint researches were conducted. METHODS: The gradient elution was applied in chromatographic separation,and 10 batches of samples from different producing areas were tested. RESULTS: Fingerprint chromatograms had a high similarity from different producing areas and batches showing 36 characteristic peaks in common. CONCLUSION: RP-HPLC is a repeatable method of controlling the fingerprint chromatograms,thus is practicable in the quality evaluation of Herpetospermum pedunculosum.Chemical components of samples from different producing areas and batches are similar,and the component ratios are stable.