ABSTRACT
Objective: To investigate the chemical constitutes of Bombyx Batryticatus. Methods: The compounds were isolated by means of solvent extraction, repeated silica gel column chromatography, ODS, and preparative RP-HPLC. The structures were identified by spectral analysis and chemical evidence. Results: Four compounds of steroids were isolated from the Bombyx Batryticatus and were identified as (3α,6β)-3-benzyl-6-isopropyl-4-methyl-2,5-morpholinedione (1), (3α,6α)-3-benzyl-6-isopropyl- 4-methyl-2,5-morpholinedione (2), 5α,8α-epidioxyergosta-6,22-dien-3β-ol (3), 22E-3β-hydroxy-5α,6α-epoxyergosta-22-en-7-one (4), 5α,6α-epoxy-(22E,24R)-ergosta-8(14),22-diene-3β,7α-diol (5), 7α-methoxy-(22E,24R)-5α,6α-epoxyergosta-8(14),22-diene-3β-ol (6), (22E,24R)-ergosta-5,7,22-trien-3β-ol (7), stigmasta-7,22-diene-3β,5α,6α-triol (8), 3β,5α-dihydroxy-(22E,24R)-ergosta-7,22-diene-6-one (9), (22E,24S)-5α,8α-epidioxy-24-methylcholesta-6,9(11),22-trien-3β-ol (10), homoarginine (11), and β-sitosterol (12). Conclusion: Compounds 1-11 are isolated from Bombyx Batryticatus for the first time.
ABSTRACT
Neurolathyrism is past history in India since Lathyrus sativus (khesari dal) is no longer used as a staple. A consensus has evolved that khesari dal is harmless as part of a normal diet. L-ODAP (β-N-oxalyl-l-α-diamino propionic acid) the neurotoxic amino acid, from this pulse, is detoxified in humans but not in animals but still no laboratory animal is susceptible to it under acceptable feeding regimens. L-ODAP is an activator of protein kinase C and consequential crucial downstream effects such as stabilization of hypoxia inducible factor-1 (HIF-1) could be extremely conducive to humans under a variety of situations. ODAP is gradually finding a place in several patents for this reason. Homoarginine the second amino acid from L. sativus can be a better substrate for endogenous generation of nitric oxide, a crucial signaling molecule associated with the cardiovasculature and control of hypertension. These features could make L. sativus a prized commodity as a functional food for the general cardiovasculature and overcome hypoxic events and is set to change the entire perception of this pulse and neurolathyrism.