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The present study attempts to study alcohol metabolizing and antioxidant properties of Gynostemma pentaphyllum(Thunb.) Makino distillate (GPD) and combination effects with Hovenia dulcis Thunb. extract (HDE) on these activities.The alcohol-metabolizing activity of GPD with/without HDE was determined by assessing alcohol dehydrogenase(ADH) and acetaldehyde dehydrogenase (ALDH) activities. To define the effect of GPD with/without HDE on alcoholmetabolism, antioxidant activities and total phenolic content of GPD with/without HD extract were evaluated using2-diphenyl-1-picrylhydrazyl free radical scavenging, ferrous chelating assays, and the Folin–Ciocalteu method.Cytotoxicity against human normal liver CHANG cells was also evaluated using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. GPD treatment alone or in combination with HDE significantly increased ADHand ALDH activities; combined treatment was most effective. Total phenolic and flavonoid contents were greater incombination than the level found in GPD alone. GPD revealed a synergistic antioxidant effect when combined withHDE. GPD and/or HDE had no antiproliferative activity against the normal liver cell line. These results suggest thatGPD-HDE combination is the possible natural resource for the management of alcohol-induced liver injury.
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Objective To study the anti-alcoholism effect of Puerariae lobatae radix, Puerariae lobatae flowers, Hovenia dulcis thunb mixture on acute alcohol poisoning in mice.Methods A model of acute alcoholism in mice was established by intragastrical administration of alcohol.The best ratio of each component in the mixture was identified by observing the sobering effect of the mixtures on mice.The best ratio mixture was divided into low dose group and high dose group.The mice tolerance time, drunken time, the ethanol concentration in the blood and the alcohol dehydrogenase activity in liver were measured to study the anti-alcoholism effect of the mixture.Results The Puerariae lobatae radix, Puerariae lobatae flowers, Hovenia dulcis thunb mixture not only increased the tolerance time and decreased the drunken time of mice, but also significantly reduced the ethanol concentration in blood and improved the activity of alcohol dehydrogenase in liver.Conclusion This study found that Puerariae lobatae radix, Puerariae lobatae flowers, Hovenia dulcis thunb mixture has anti-alcoholism effect on acute alcoholism in mice.
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Objective: To investigate the nootropic components of Hovenia dulcis. Methods: Six saponins were isolated from Hovenia dulcis and Step-down test was used to examine the memory ability of mice. The escape latency and the times of wrong performance within 3 min were used to evaluate the memory ability of mice. To study the effects of saponins on learning and memory in mice, we divided the mice into 6 groups: youth group, aged group, aged plus piracetam(0.3 g/kg) group, aged plus saponins (0.6 g/kg) group, aged plus saponins (0.3 g/kg) group, and aged plus saponins(0.15 g/kg) group. To study the influence of saponins on impairment of memory acquirement, consolidation, and reoccurrence (induced by scopolamine, sodium nitrite and 40% ethanol, respectively), mice were also divided into the following 7 groups: control group, untreated group, Piracetam group (0.3 g/kg), compound 3 group(0.3 g/kg), compound 4 group(0.3 g/kg), compound 5 group(0.3 g/kg), and compound 6 group (0.3 g/kg). Results: The chemical structures of six saponins were elucidated as 3-O-stigmasterol-(6-O- palmitoyl)-β-D-glucopyranoside (1), β-daucosterin (2), hovenidulcioside A1 (3), hoduloside I (4), hoduloside IV (5), and saponins C2 (6). Among them, compounds 1 and 2 were isolated from this plant for the first time. Compounds 5 and 6 had enhancing effect on the learning and memory ability of natural senile mice, and they could improve the impairment of memory acquirement, consolidation and recurrence in mice induced by scopolamine, sodium nitrite and 40% ethanol, respectively. Conclusion: The aglycone of jujubogenin might be the main saponins contributing to the nootropic effect of total saponins from Hovenia dulcis.
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OBJECTIVE:To study the active fraction of the seeds of Hovenia Dulcis Thunb.to treat acute alcoholism.METHODES:The seeds of Hovenia Dulcis Thunb.were divided into five parts:superitical fluid extraction(SFE)part,chloroform extraction part,ethyl acetate extraction part,n-butanol extraction part and water extraction part.The effects of the active fraction of the seeds of Hovenia Dulcis Thunb.on ebriety latency,ebriety rate,mortality,and ethanol concentration-time curve of the alcoholism mice were investigated.RESULTS:The ethyl acetate extract could prolong the ebriety latency of the mice,decrease ebriety rate and mortality of the alcoholism mice,and significantly decrease serum ethanol concentrations at 0.5,1,and 1.5h after alcohol administration(P
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Objective To establish a quality standard of Hovenia dulics Thunb. Method The presence of quercetin was identified and assayed by TLC and HPLC, respectively. Result Linearity of marker was obtained over the range of 1.32 ～ 5.28 ?g (r =0.9999). The average recovery rate was 98.78% (RSD=1.08%). Conclusion TLC is specific. The method of quality is accurate, reappearance, simple, rapid, and suitable for the quality control of Hovenia dulcis Thunb.
ABSTRACT
Objective:To investigate the nootropic components of Hovenia dulcis.Methods: Six saponins were isolated from Hovenia dulcis and Step-down test was used to examine the memory ability of mice.The escape latency and the times of wrong performance within 3 min were used to evaluate the memory ability of mice.To study the effects of saponins on learning and memory in mice,we divided the mice into 6 groups:youth group,aged group,aged plus piracetam(0.3 g/kg) group,aged plus saponins(0.6 g/kg) group,aged plus saponins(0.3 g/kg) group,and aged plus saponins(0.15 g/kg) group.To study the influence of saponins on impairment of memory acquirement,consolidation,and reoccurrence(induced by scopolamine,sodium nitrite and 40% ethanol,respectively),mice were also divided into the following 7 groups:control group,untreated group,piracetam group(0.3 g/kg),compound 3 group(0.3 g/kg),compound 4 group(0.3 g/kg),compound 5 group(0.3 g/kg),and compound 6 group(0.3 g/kg).Results: The chemical structures of six saponins were elucidated as 3-O-stigmasterol-(6-O-palmitoyl)-?-D-glucopyranoside(1),?-daucosterin(2),hovenidulcioside A1(3),hoduloside Ⅰ(4),hoduloside Ⅳ(5),and saponins C2(6).Among them,compounds 1 and 2 were isolated from this plant for the first time.Compounds 5 and 6 had enhancing effect on the learning and memory ability of natural senile mice,and they could improve the impairment of memory acquirement,consolidation and recurrence in mice induced by scopolamine,sodium nitrite and 40% ethanol,respectively.Conclusion: The aglycone of jujubogenin might be the main saponins contributing to the nootropic effect of total saponins from Hovenia dulcis.