ABSTRACT
Objective To investigate the clinical application of Huashi-Xingyu-Qingre decoction for the treatment of oral lichen planus (OLP).Methods According to the random table method,83 OLP patients were divided into control group (n=41) and the research group (n=42).The patients in the control group were treated with conventional medicine,while the patients in the research group were treated with Huashi-Xingyu-Qingre decoction on the basis of control group.Two groups of patients were treated for 6 weeks.The clinical total effective rate of two groups after treatment was counted.The serum haptoglobin,vitamin D binding protein,AT-Ⅲ,IL-6,IL-8,TNF-α and peripheral blood CD3+,CD4+,CD4+/CD8+ were determined respectively before and after treatment.The adverse reaction incidence and recurrence rate of two groups were observed.Results The total effective rate of research group was 92.9％ (39/42),while the total effective rate of control group was 63.4％ (26/41),and the difference between two groups was statistically significant (22=10.589,P=0.001).After treatment,the serum haptoglobin (6.11 ± 0.72 μg/ml vs.5.58 ± 0.69 μg/ml,t=3.423) of research group were significantly higher than the control group (P＜0.05),while the serum vitamin D binding protein (1.48 ± 0.25 μg/ml vs.2.06 ± 0.31 μg/ml,t=9.394),AT-Ⅲ,IL-6,1L-8,TNF-α of research group were significantly lower than the control group (t were 17.561,12.664,7.423,13.763,P＜0.01),and the peripheral blood CD3+,CD4+,CD4+/CD8+ of research group were significantly higher than the control group (t were 5.368,4.694,3.558,P＜0.01).The recurrence rate of control group was 30.8％ (8/26),which was significantly higher than the research group of 7.7％ (3/39) (x2=5.909,P=0.015).Conclusions The clinical curative effect of Huashi-Xingyu-Qingre decoction on the treatment for OLP is significantly,which can improve the patient's serum differential proteins and inflammatory cytokines,improve the body's immune function.It has the high security of the forward curative effect,which provides new thinking for clinical treatment of CFS.
ABSTRACT
Objective Oral lichen planus ( OLP) is a common complaint in the oral mucosa , for which there is no definite therapy hitherto.This article aimed to investigate the possible effect of Huashi Xingyu Qingre Decoction (HXQD) on OLP. Methods This study included 30 randomly selected cases of OLP treated with HXQD .Fasting venous blood and total serum protein were obtained before and after medication for screening and identification of OLP-related differential proteins by two-dimensional fluorescence differ-ence gel electrophoresis (2D DIGE) and liquid chromatography-mass spectrometry (LC-MS), followed by Western blot validation . Results Haptoglobin , antithrombin Ⅲ, C1 complement , and vitamin D binding protein were differentially expressed in the serum of the OLP patients before and after treated with HXQD .Compared with the baseline , the expression of haptoglobin was significantly in-creased (103.086 ±27.536 vs 159.704 ±24.228, P<0.05) while that of antithrombin Ⅲ remarkably decreased after treatment (150.00 ±54.04 vs 98.00 ±28.04, P<0.05). Conclusion Haptoglobin, antithrombin Ⅲ, C1 complement, and vitamin D binding protein are differentially expressed in the serum of OLP patients before and after HXQD medication , which may be associated with the development and progression of OLP .