ABSTRACT
Objective: To investigate the effect of total glycosides from Ranunculus japonicus (TGRJ) on vasculogenic mimicry of human gastric cancer MGC803 cell line in vitro and its mechanisms. Methods: The effect of TGRJ on the cell adhesion of MGC803 was observed by cell attachment assay. The effect of TGRJ on the cell migration of MGC803 was studied by cell wound healing assay. The function of TGRJ on the tube-like structures formation of MGC803 in vitro was verified by tube formation assay. The effect of TGRJ on the expression of vasculogenic mimicry related genes was assessed by semi-quantitative RT-PCR assay. Results: TGRJ effectively inhibited the cell adhesion, cell migration of MGC803, and the MGC803-mediated vasculogenic mimicry in vitro in a dosage-dependent manner. Furthermore, the semi-quantitative RT-PCR showed that TGRJ markedly inhibited the expression of MGC803 vasculogenic mimicry-related genes VE-cadherin, sema 4D, and integrin β5. Conclusion: TGRJ could effectively inhibit the cell attachment and cell migration of MGC803, and the MGC803-mediated vasculogenic mimicry by down regulating vasculogenic mimicry-related genes VE-cadherin, sema 4D, and integrin β5.