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ABSTRACT:Objective To explore the effects of virus interleukin‐10 (vIL‐10 ) on different expressions of MHC‐I antigen processing “the operon” .Methods We collected nasopharyngeal carcinoma cells (CNE‐1 and CNE‐2) treated by vIL‐10 at different time points ,and detected the changes of MHC‐I antigen processing “the operons” (TAP‐1 ,TAP‐2 ,LMP‐2 ,LMP‐7 and HLA‐I) by RT‐PCR and Western blot .Results ① mRNA level :There was no difference in the expression of TAP‐1 in CNE‐1 and CNE‐2 cells at various time points .The expressions of TAP‐2 and LMP‐2 in CNE‐1 and CNE‐2 did not change at 1 ,4 ,6 ,12 h ,but downregulated and even disappeared at 24 h .The expression of LMP‐7 in CNE‐1 decreased 4 h after vIL‐10 was added ,and that in CNE‐2 decreased at 6 h .The expression of HLA‐I in CNE‐1 and CNE‐2 showed significant decrease at 24 h .② Protein expression :The expression of TAP‐1 in CNE‐1 and CNE‐2 showed significant decrease at 24 h .The expression of TAP‐2 in CNE‐1 and CNE‐2 was gradually downregulated at different time points .The expressions of LMP‐2 and LMP‐7 in CNE‐2 were gradually downregulated at different periods ,while that in CNE‐1 was only decreased at 12 h .The expression of HLA‐I in CNE‐1 and CNE‐2 was gradually downregulated ,but there was no significant difference at each period in CNE‐1 ,while the expression of HLA‐I in CNE‐2 at 24 h was significantly downregulated .Conclusion vIL‐10 can inhibit the expression of MHC‐I antigen processing “the operon” in NPC in the time‐dependent manner .
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Objective: To identify the differential expression of human leukocyte antigen I (HLA-I) between patients of Uighur and Han ethnic groups with cervical diseases. Methods: The expression levels of HLA-I protein in chronic cervicitis, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC) tissues from female patients of Uighur and Han ethnic groups were examined by immunohistochemistry method. Results: The expressions of HLA-I protein in chronic cervicitis, CIN and CSCC tissues were normal, partial-lost and negative, respectively. The total loss rates of HLA-I protein expression were 2% (1/49), 26% (29/113) and 49% (34/70) in chronic cervicitis, CIN and CSCC tissues, respectively (P<0.05). The increasing tendency of total loss rate of HLA-I protein expression accompanied by the enhancement of cervical disease severity in the Uighur ethnic group was similar to that of the Han ethnic group. The total loss rates of HLA-I protein expression in CIN and CSCC tissues were both higher in Uighur women (27% and 53%, respectively) than those in Han women (18% and 37%, respectively). Conclusion: The loss of HLA-I protein expression may predict the progression of cervical cancer. The differential expression of HLA I between Uighur and Han ethnic groups may reflect the different genetic pathways of carcinogenesis.