ABSTRACT
Cervical cancer is one of the most common gynecological malignancies which seriously threatens the health of women. How to further expand the scope of screening and improve the sensitivity and specificity of screening and optimize screening programs are the key points of cancer prevention and treatment. At present,the screening methods that are commonly used at home and abroad include cervical smear cytology, human papillomavirus ( HPV) detection,molecular biology detection and genetic genetic testing. Cervical smear cytology has high specificity and low sensitivity,the sensitivity of HPV detection is high,but the specificity is low,and the molecular biological detection and genetic testing has high specificity and high sensitivity,but due to the lack of large quantities of clinical samples,and the higher detection cost,they are still in the test study stage.
ABSTRACT
Objective To investigate the correlation between the human telomerase gene (hTERC) and the generation and progression of cervical cancer.Methods A total of 173 patients with abnormal results of cervical biopsy by colposcopy were selected,meanwhile the 40 normal cases were selected as control group.The expression of hTERC gene was detected by fluorescence in situ hybridization (FISH),and the relationship between the HPV infection and the positive rate of hTERC was analyzed.Results The positive rate of hTERC in the control group was 7.50％,which was respectively 15.79％,46.15％,68.63％,and 91.11％ in the CIN Ⅰ group,CIN Ⅱ group,CIN Ⅲ group,and cervical cancer group.The positive rate of the hTERC was significantly increased during the progression of cervical exfoliative cell lesions (P ＜ 0.05).The precancerous lesion and cancer rate in patients with high-risk HPV infection and positive hTERC was 100％,significantly higher than that of patients with high-risk HPV infection as well as positive hTERC and also non high-risk HPV infected patients (P ＜ 0.05).Conclusion hTERC gene amplification is involved in the development of cervical cancer,and increase with cervical lesions rising,and the positive expression of hTERC gene is valuable to the early diagnosis of cervical cancer.