ABSTRACT
Objective To investigate the infection of Coxiella burnetii (C. burnetii) in Hyalomma asiaticum (Hy. asiaticum) from Northern Xinjiang, China, and to provide basis for reducing the incidence of Q fever in humans and livestock. Methods Questing ticks were collected via the dragging-flag method and directly sampled from animals in Fuhai County, Alataw Pass, Shihezi City, Hutubi County and Karamay City between April and July in 2016. Genomic DNA was extracted after morphological identification. PCR was used to identify tick species and Coxiella pathogen. BLAST and Mega 6.0 were used in the sequence analysis and the construction of phylogenetic trees. Results A total of 822 ticks were collected from 5 counties (cities) in Northern Xinjiang, among which the dominant ticks were Hy. asiaticum, and 580 were collected. Hy. asiaticum was sequenced, and BLAST results showed that the sequence had a high similarity with that of Yuli County from Xinjiang (accession no. KF527439). The nucleotide of C. burnetii was amplified via the PCR method, and its positive rate was 19.48% (113/580). Blast analysis showed that positive sequences had a high similarity with that of C . burnetii (99.66%, 296/297). Phylogenetic tree showed that the sequences of C. burnetii had genetic diversity. Conclusions C. burnetii extensively exists in Hy. asiaticum from 5 counties (cities) in Northern Xinjiang. These results suggest Q fever survey should be strengthened in different regions of Xinjiang.
ABSTRACT
We conducted the detection the Francisella spp.nucle acid from Hyalomma asiaticum asiaticum that main distribution is on railway line area from China-Kazakhstan border.The free-living ticks were collected and then identified by morphological and molecular methods.After species identification,they were detected by PCR targeting 16S rRNA and sdhA of Francisella spp.The amplified products were sequenced and the sequences was analyzed by using the Blast.A phylogenetic tree was constructed using MEGA 6 software.A total of 243 fleas were identified as H.asiaticum asiaticum.Only 35 samples were detected for Francisella spp.positive and the positive rate was 14.4％.Sequence analysis showed that two different sequences (seql and seq2) and all belong to Francisella-like endosymbionts (FLEs).Phylogenetic analyses showed that two FLEs were belong to the same cladd.This is first detection of FLEs nucleic acid from H.asiaticum Railway line area of China-Kazakhstan border.
ABSTRACT
Objective To investigate the infection state and genotype of Babesia from tick at Alataw port,the China-Kazakhstan border.Methods Drag-flag method and animal body surface method were used to collect ticks at Ebinur Lake wetland,Alataw port.18s rRNA gene of Babesia was tested by polymerase chain reaction (PCR),the sequence analysis was conducted with Blast and phylogenetic analysis was conducted with Mega 6.0.Results The positive rate of Babesia gene in ticks was 12.65％ (32/253) at Alataw port.By sequencing,32 sequences were divided in ALSK174,ALSK191,ALSK019 three groups.Analysis of Blast showed that ALSK174 had the highest homology with Babesia caballi (EU888904,South Africa),it was 99.72％ (356/357);ALSK191 had the highest homology with Babesia occultans (KP745626,Turkey),it was 99.72％ (350/351);ALSK019 had 95.76％ (339/354) homology with Babesia odocoilei (KC460321,Canada).Conclusion In this study,we have first reported that the Babesia is infected from ticks at Alataw port,the China-Kazakhstan border.
ABSTRACT
Gynandromorphic ticks are extremely rare, and often attract parasitologists' attention. During our examination of tick specimens, an engorged gynandromorph of Hyalomma asiaticum was noticed. This is the first record of gynandromorphic ticks from China. In this study, several important morphological structures of normal and gynandromorphic H. asiaticum were analyzed. Comparing to the normal H. asiaticum, the gynandromorphic specimen was a typical bipartite protogynander. Its right side showed normal female characteristics, whereas the left side had normal male traits. Different from other gynandromorphic ticks containing 1 anus, this tick reported here had 2 complete anuses, and the anus of the male part had a single adanal plate.
Subject(s)
Animals , Female , Male , Chimera/anatomy & histology , China , Ixodidae/anatomy & histology , Sheep , Sheep Diseases/parasitology , Tick Infestations/parasitologyABSTRACT
The paper reports the isoenzyme patterns of Malic dehydrogenase, Esterase and Lactic dehydrogenase of the ixodid tick Haemaphysalis longicornis, Hyalomma asiaticum asiaticum and H. detrilum.H. longicornis:Malic dehydrogenase isoenzyme of the male, female,nymph and larva consists of 5, 6, 5 and 4 bands respectively.Esterase isoenzyme of the male, female, nymph and larva consists of 5, 5, 5 and 8 bands respectively.Lactic dehydrogenase isoenzyme of the male, female, nymph and larva consists of 3, 2, 2 and 2 bands respectively.H. asiaticum asiaticum:Malic dehydrogenase isoenzyme of the male, female, larva and egg consists of 3, 3,5 and 9 bands respectively.Esterase isoenzyme of the male, female, larva and egg consists of 6, 6, 6 and 3 bands respectively.Lactic dehydrogenase isoenzyme of the male, female, larva, and egg consists of 2, 2, 2 and 2 bands respectively.H. detritum:Malic dehydrogenase isoenzyme of the egg consists of 8 bands. Esterase isoenzyme of the egg consists of 5 bands. Lactic dehydrogenase isoenzyme of the egg consists of 2 bands