ABSTRACT
Objective To compare the cardioprotective efficacy of Cariporide as an adjunct in different pH cardioplegia. Methods Sixty-four male Sprague-Dawley rat hearts were randomly divided into 8 groups (8 rats in each group): 4 control groups and 4 treated groups with Cariporide as an adjunct to cardioplegia. After control perfusion in Langendorff mode with Krebs-Henseleit bicarbonate buffer (KHB) for 30 minutes, the isolated rat hearts were infused with different pH (pH=6.2 or 7.0 or 7.4 or 7.8, respectively) cardioplegia (without or with Cariporide) for 2 minutes to produce cardiac arrest and subjected to 60 minutes of 15?C arrest. In addition, during global ischemia, cardioplegia was reinfused for 2 minutes every 30 minutes, Sixty minutes after the ischemic arrest, cardioplegia was infused as terminal cardioplegia for 2 minutes, then the hearts were reperfused with Krebs-Henseleit bicarbonate buffer for 30 minutes. The hemodynamic parameters and the level of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) of coronary venous sinus drainage were measured before ischemia and during reperfusion. Myocardial and mitochondrial ultrastructures were observed under electronmicroscope. Results Application of Cariporide as an adjunct to cardioplegia improved significantly recovery of cardiac function and decreased the level of creatine kinase-MB(CK-MB), lactate dehydrogenase (LDH) of coronary venous sinus drainage (P
ABSTRACT
Objective To set up a system in vitro to rapidly recover plasma proteins lost during artificial-liver treatment.Methods The polyprotein precipitation was obtained by all proteins whose isoelectric point pH value were between 7.3 and 5.1,which collided with each other and aggregated using gradient pH co-precipitation(adding 1 mol/L citric acid slowly in the plasma solution to change the pH values gradually from 7.3 to 5.1 in 5 h)combined with salting out(degree of saturation of NaCl is 33%,reacted for 5.5 h at 4℃)or low-temperature ethanol precipitation(40% ethanol, reacted for 5.5 h at -7℃)so that to get rid of toxicants by discarding the supernatant.Results In the range of pH 5.1-7.3,50%(29g/57g)of the total plasma proteins had been recovered by the gradient pH salting out and 41%(25 g/61g)by the gradient pH low-temperature ethanol co-precipi- tation.The protein remained in the supernatant was mostly albumin and its combined bilirubin.The levels of total bilirubin decreased to 0.07% and 0.06% of the original levels by these two methods respectively and the serum HBV DNA level decreased to be undetected(quantitative PCR).Conclu- sions The proteins with close isoelectric point can co-precipitated with the presence of high concen- tration of NaCl or low-temperature ethanol and by changing the pH value gradually.The total protein in the discarded plasma during artificial-liver treatment can be recovered rapidly using the gradient pH coprecipitation.