Interleukin-10 Gene -592 Promoter Polymorphism in Patients with Endometriosis / 대한산부인과학회지

OBJECTIVE: To explore the association of the interleukin-10 (IL-10) gene -592 promoter polymorphism with endometriosis in a Korean population. METHODS: This study comprised 254 women with surgically or histologically diagnosed endometriosis, 236 control women with no evidence of endometriosis by laparoscopy or laparotomy. Following extraction of genomic DNA, genotyping of the IL-10 gene -592 polymorphism was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. RESULTS: The distribution of genotypes and allele frequencies of IL-10 gene -592 polymorphism in the endometriosis group did not differ from those in the control group (A/A: A/C: C/C, 41.3%: 51.2%: 7.5% vs. 44.9%: 50.8%: 4.2%). And when classified by stage, there was also no significant difference in the distribution of IL-10 gene -592 polymorphism between patients with stage I-II (ASRM, 1997) endometriosis or patients with stage III-IV endometriosis and controls. CONCLUSION: These results suggest that IL-10 gene -592 promoter polymorphism is not associated with the risk for endometriosis in the Korean women.

The Construction of Lentiviral-delivery Human Interleukin-10 Expression Vector and the Abirritative Effect of Pain on CCI Rats / 中国生物工程杂志

Objective:To construct the lentiviral-vector encoding human interleukin-10 protein(LV-hIL-10) and to observe the effect of LV-hIL-10 on controlling neuropathic pain via intrathecal administration in CCI rats. Methods:hIL-10 gene fragment was isolated and amplified from pCYIL-10 plasmid by PCR, and was cloned into pWPXL. The recombinant plasmid pWPXL-IL-10,envelope plasmid pMD2.G and packaging plasmid psPAX2 were cotransfected into 293T cells, LV-hIL-10 is prepared by concentrating the collected supernatant .At the same time, the empty plasmid pWPXL-GFP,pMD2.G and psPAX2 were cotransfected into 293T cells, LV-GFP is prepared for contrast.135 sheer breed pathogen-free adult male Sprague-Dawley rats divided into 9 arrays at random: CCI models 4 arrays (C0,C1,C2,C3), sham operatived rats 4 arrays (S0,S1,S2,S3) and a normal contrast array (N), each respectively intrathecal injection LV-hIL-10 (C1,S1)、LV-GFP (C2, S2),isotonic Nachloride (C3,S3) and control (no implanted catheters and no administration, C0,S0), the pain threshold of each array and the expression of mRNA and protein of IL-10 in spinal cord,pallium and hippocampus on different time were observed after intrathecal administration LV-hIL-10 in successful CCI model rats . Results:The hIL-10 gene fragment was obtained from pCYIL-10 plasmid, pWPXL-hIL-10 was recombinated successfully. the cloned gene segment was validated by DNA sequencing .High titer(2?1010)and highly purified LV-hIL-10 particles were obtained by three plasmids were cotransfected into 293T cells. The mechanical allodynia and thermal hyperalgesia were alleviated via intrathecal injection LV-hIL-10 in CCI rats. The overexpression of IL-10 were detected in spinal cord,pallium and hippocampus , especially in the spinal cord .Conclusions:The mechanical allodynia and thermal hyperalgesia can be relieved by intrathecal injection LV-hIL-10 in CCI rats.

Interleukin-10 Gene Polymorphism in patients with Schizophrenia / 신경정신의학

OBJECT: This study was aimed at comparing the Interleukin-10 gene polymorphic variants of patients with schizophrenia with those of normal controls, to investigate its contribution to the development of schizophrenia. METHOD: Two hundred and thirty-three patients with schizophrenia in accordance with DSM-IV criteria and 181 healthy individuals participated in this study. DNA was extracted from whole blood using proteinase K, and the interleukin(IL)-10 gene promoter region was amplified by polymerase chain reaction(PCR). Gene typing was analyzed by restriction fragment length polymorphism(RFLP) and single strand conformation polymorphism(SSCP). RESULTS: Distributions of the alleles and haplotypes of IL-10 gene in patients with schizophrenia were not significantly different from those of controls. There was no difference in the frequencies of alleles and haplotypes between patients with paranoid subtype and with non-paranoid subtype. CONCLUSION: In this study, we found no relataionship between IL-10 gene polymorphic variants and schizophrenia. Further systemic studies including adjacent genes and diverse clinical variables may reveal the effects of IL-10 gene on the susceptibility to schizophrenia.

Restriction Fragment Length Polymorphism of Interleukin-10 Gene in Major Depression

OBJECTIVE: Major depression is known to have immunologic dysfunctions, the recent studies revealed that cytokines including Il-6 and IL-1beta were increased in patients with major depression. Since molecular genetic methods have been progressed, this study was to investigate the relationship between major depression and immunologic aspects by analyzing polymorphism of IL-10 gene. METHOD: 92 patients with major depression were included and data of 146 normal controls obtained from the Catholic Hemopoietic Stem Cell Information Bank of Korea were used in this study. DNA was extracted from whole blood, thereafter amplified by polymerase chain reaction, and digested by Mae III. After that procedure, we obtained and assessed RFLP of two alleles, IL-10T and IL-10C. All data were analyzed by chi2 test. RESULTS: 1) There were no significant difference in genotype frequencies of IL-10*T/T, IL-10*T/C, and IL-10*C/C between major depression patients group and control group. 2) There were no significant difference in allelic frequencies of IL-10*T and IL-10*C between major depression patients group and control group. CONCLUSION: We did not verified the differences in frequencies of IL-10*T/IL-10*C gene between the major depression patients group and control group, respectively. But the results of this study do not declare that the IL-10 gene has no association with major depression. We do suggest that further systematic studies including various clinical variables should be conducted.

Restriction Fragment Length Polymorphism of Interleukin-10 Gene in Schizophrenia / 신경정신의학

OBJECTIVE: Recently, molecular genetic methods have been progressed, this study was to investigate the relationship between schizophrenia and immunologic aspects by analyzing polymorphism of IL-10 gene, which is involved in interaction of immunologic system and CNS. METHOD: 141 schizophrenic patients diagnosed by DSM-IV were included and data of 146 normal controls obtained from the Catholic Hemopoietic Stem Cell Information Bank of Korea were used in this study. DNA was extracted from whole blood, thereafter amplified by polymerase chain reaction, and digested by Mae III After that procedure, we obtained and assessed RFLP of two alleles, IL-10T and IL-10C. All data were analyzed by x2 test with two-tailed Fisher's exact test. RESULTS: 1) There were no significant differences genotype frequencies of IL-10*T/T, IL-10*T/C, and IL-10*C/C in between schizophrenic patients group and control group. 2) There were no significant differences gene frequencies of IL-10*T and IL-10*C in between schizophrenic patients group and control group. CONCLUSION: We did not verified the frequency differences of IL-10*T/*IL-10*C gene between schizophrenic patients and normal controls, respectively. We do suggest that further systematic studies including various clinical variables should be conducted.