ABSTRACT
Chinese materia medica (CMM) fingerprint technology is an effective method to evaluate pros and cons, identify the authenticity, distinguish species, and ensure consistency and stability of CMM. With the development of modern analytical technology, CMM fingerprint technology is widely used and approved in the study of effective component, quality control, and identification of Chinese medicine. The related references of biological fingerprint of CMM and chemical fingerprint of CMM (including IR, UV, NMR, electro-chemical method, TLC, HPLC, GC, and CEP) and data calculation methods in the three years has been summarized in this article. Meanwhile, the direction and prospect of the CMM fingerprint technology are discussed.
ABSTRACT
Accurate identification of varieties is the most important part of quality control of Chinese materia medica (CMM). To find an efficient, convenient, and accurate identification method is the development trend of identification technology of CMM. Compared with the traditional identification method based on phenotypic markers, DNA molecular marker technology is more accurate and reliable, suitable for the identification of closely related species and sample with confusion and multiple sources, but unable to realize the rapid identification due to the limits of the PCR technology, such as high cost, complex procedures, and the drawback of long time. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technology, with the advantages of high specificity, high sensitivity, simple, rapid, low cost, etc., become another new technology after PCR to realize the rapid molecular identification of CMM successfully. In this paper, the common isothermal amplification of nucleic acid technology and its application in the study of molecular identification of Chinese herbal medicines were reviewed analysis, to provide a reference for the study of rapid molecular identification system of Chinese herbal medicines.