Isolation and Identification of Bioactive Constituents from Stem Barks of Illicium difengpi / 中草药·英文版

Objective: To isolate and identify bioactive constituents from the stem barks of Illicium difengpi. Methods: The chemical constituents were isolated and purified by repeated silica gel, Sephadex LH-20, recrystallization, and preparative HPLC techniques. The structures of the compounds were identified on the basis of spectral data including NMR, MS, and IR. Results: Two sesquiterpene lactones, majucin (1) and anisatin (2), two steroids, β-sitosterol (3) and daucosterol (4), three carboxylic acids, 2-ethyldecanoic acid (5), shikimic acid (6), and 3,4-dihydrobenzoic acid (7), and a flavonoid, quercetin (8), were successively isolated from the stem barks of I. difengpi. Conclusion: Apart from compound 3, other seven compounds are reported in this plant for the first time. The results suggested that the current studies on I. difengpi is still far from being well known and therefore more studies need to be done for better understanding of this plant. © 2014 Tianjin Press of Chinese Herbal Medicines.

Chemical constituents of Illicium brevistylum / 中草药

Objective: To isolate and identify the chemical constituents in the ethanol extract from the barks of Illicium brevistylum. Methods: The barks of I. brevistylum were extracted by 80% ethanol then partitioned by system solvents with different polarity. The ethyl acetate extract was separated on silica gel, Sephadex LH-20, and ODS columns. The isolated compounds were identified by physicochemical properties and spectral analyses. Results: Twelve compounds were isolated and purified from the ethyl acetate extract of I. brevistylum and the structures were identified as quercetin 3-O-β-xylopyranoside (1), quercetin (2), dihydro kaempferol (3), kaempferol (4), wulingzhic acid V (5), catechin (6), 3, 5, 7-trihydroxylchromone-3-O-α-L-arabinopyranoside (7), majucin (8), anislactone B (9), 7-O-acetylanislactone B (10), dunnianolide C (11), and taxifolin-3-O-β-xylopyranoside (12). Conclusion: Compounds 5 and 7 are obtained from the plants in Illiciaceae for the first time and compounds 1, 6, and 8-11 are firstly isolated from I. brevistylum.