ABSTRACT
AIM To study the chemical constituents from Illicium brevistylum A.C.Smith and to evaluate their anti-inflammatory activities.METHODS The n-BuOH fraction of 80％ ethanol extract from I.brevistylum was isolated and purified by silica,ODS and Sephadex LH-20,the structures of obtained compounds were identified by spectral data.Then their anti-inflammatory activities were screened.RESULTS Nine compounds were isolated and identified as (7S,8R)-3,3',5-trimethoxy-4',7-epoxy-8,5'-neolignan-4,9,9'-triol (1),methylabieta-8,11,13,15-tetraen-18-oate (2),majusanin A (3),pubeside C (4),(+)-lyoniresinol-3a-O-α-L-rhamnopyranoside (5),junipercomnoside D (6),lyoniside (7),nudiposide (8),rhyncoside A (9).The inhibition rates of compounds 2,6,9 on NF-κB were 47.81％,37.33％,33.37％,respectively.CONCLUSION Compounds 1,5,7-9 are isolated from genus Illicium for the first time,and compounds 2,6,9 exhibit good anti-inflammatory activities.
ABSTRACT
AIM To study the chemical constituents from Illicium brevistylum A.C.Smith and to evaluate their anti-inflammatory activities.METHODS The n-BuOH fraction of 80％ ethanol extract from I.brevistylum was isolated and purified by silica,ODS and Sephadex LH-20,the structures of obtained compounds were identified by spectral data.Then their anti-inflammatory activities were screened.RESULTS Nine compounds were isolated and identified as (7S,8R)-3,3',5-trimethoxy-4',7-epoxy-8,5'-neolignan-4,9,9'-triol (1),methylabieta-8,11,13,15-tetraen-18-oate (2),majusanin A (3),pubeside C (4),(+)-lyoniresinol-3a-O-α-L-rhamnopyranoside (5),junipercomnoside D (6),lyoniside (7),nudiposide (8),rhyncoside A (9).The inhibition rates of compounds 2,6,9 on NF-κB were 47.81％,37.33％,33.37％,respectively.CONCLUSION Compounds 1,5,7-9 are isolated from genus Illicium for the first time,and compounds 2,6,9 exhibit good anti-inflammatory activities.
ABSTRACT
Objective: To isolate and identify the chemical constituents in the ethanol extract from the barks of Illicium brevistylum. Methods: The barks of I. brevistylum were extracted by 80% ethanol then partitioned by system solvents with different polarity. The ethyl acetate extract was separated on silica gel, Sephadex LH-20, and ODS columns. The isolated compounds were identified by physicochemical properties and spectral analyses. Results: Twelve compounds were isolated and purified from the ethyl acetate extract of I. brevistylum and the structures were identified as quercetin 3-O-β-xylopyranoside (1), quercetin (2), dihydro kaempferol (3), kaempferol (4), wulingzhic acid V (5), catechin (6), 3, 5, 7-trihydroxylchromone-3-O-α-L-arabinopyranoside (7), majucin (8), anislactone B (9), 7-O-acetylanislactone B (10), dunnianolide C (11), and taxifolin-3-O-β-xylopyranoside (12). Conclusion: Compounds 5 and 7 are obtained from the plants in Illiciaceae for the first time and compounds 1, 6, and 8-11 are firstly isolated from I. brevistylum.