ABSTRACT
Ten seco-prezizaane sesquiterpenes were isolated from the water-soluble fraction of the fruit of Illicium lanceolatum using the combined methods of silica gel column chromatography,Sephadex LH-20 column chromatography,and RP-preparative HPLC. They were elucidated as majusanol E( 1),2α-hydroxycycloparviflorolide( 2),2β-hydroxy-3,6-dedioxypseudoanisatin( 3),majusanol A( 4),merrillianone( 5),cycloparvifloralone( 6),3α-hydroxycycloparvifloralone( 7),1,2-dehydrocycloparvifloralone( 8),henrylactone C( 9),and( 11) 7,14-ortholactone-3α-hydroxyfloridanolide( 10) according to the NMR data. All compounds were obtained from this plant for the first time. Neuroprotection activity,anti-Coxsackie B3 virus,and anti-H3 N2 virus experiments were carried out to test their bioactivities. The bioassay results showed that compounds 1,4,6,7,9 and 10 displayed weak protective effects of the damage of nerve SH-SY5 Y cell induced by monosodium glutamate.
Subject(s)
Fruit , Illicium , Magnetic Resonance Spectroscopy , Molecular Structure , Neuroprotection , SesquiterpenesABSTRACT
Illicium henryi Diels is a medicinal and aromatic plant native to China with Chinese name Mancao or Hondu-hui.The name of Mancao was first recorded in the"Classic of Mountains and Rivers"and the earliest documented medicinal function of this plant was found in"Shen Nong′s herbal classic".Its botanical origin includes Illicium lanceolatum A.C. Smith and Illicium henryi Diels while Miscanthus sinensis Anderss(Gramineae)and Tripterygium wilfordii Hook.f. (Celastraceae)were recorded as Mancao by mistake.Mancao is mainly distributed in Henan,Hebei,Sichuan,Zhejiang and Fu-jian Province.All those plants are toxic.In this paper,the name,habitats,origins,the characteristics and effects of Illicium lanceolatum A.C.Smith and Illicium henryi Diels were investigated to correct the origin of the textural records and provide a reference for future research,development and utilization of the medical resources.
ABSTRACT
OBJECTIVE: To study the chemical constituents from the fruits oi Illicium lanceolatum. METHODS: The fruits oil. lanceolatumwere extracted with 95% ethanol. The ethanol extract was subjected to silica column, Sephadex LH-20 and MCI chromatography. The isolated compounds were identified on the basis of spectral analyses including MS, H-NMR, and C-NMR. RESULTS: Twelve compounds were isolated, which were characterized as 2, 4-dihydroxy-allylbenzene-2-O-β-D-gluco-pyranoside(1), (+) -catechin (2), quercetin-3-O-β-L-rhamnopyranoside (3), rutin (4), isorhamnetin-3-O-β-D-rutinoside (5), 6-deoxy-1-hydroxy neoanisatin (6), anisatin(7), icariside E3 (8), (75, 8R)-urolignoside (9), 4-hydroxy acid benzoic acid(10), shikimic acid(11) and β-sitosterol(12). CONCLUSION: Compounds 1, 4-6, and 8-10 are obtained from I. lanceolatum for the first time.