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Objective To make quantitative analysis on collected cell images combined with machine learning integrated clustering algorithm, so as to explore a method for fast recognition and classification of cells in mixed cultures based on morphology. Methods The morphometric properties of A549 and 3T3 cells in vitro were characterized by immunostaining, the fluorescent images were then analyzed with CellProfiler to extract the parameters of cell morphology. The parameters were loaded into CellProfiler Analyst to be trained with machine learning algorithm, and a rule was developed to form a generalization capability for cell classification in mixed cultures. Results The accuracy of the training classifier was 81-24%, and the binary classifications of A549 and 3T3 cells could be realized. Conclusions The method of machine learning is very effective in parameter clustering. The application of machine learning into cell image recognition can provide pre-judgment for rapid pathological examination of tissue sections, thereby reducing the workload of doctors and improving the accuracy of diagnosis.
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BACKGROUND@# Point-of-care ultrasound has gained widespread use in developing countries due to decreased cost and improved telemedicine capabilities. Ultrasound training, specifically image acquisition skills, is occurring with more frequency in non-medical personnel with varying educational levels in these underdeveloped areas. This study evaluates if students without a high school education can be trained to acquire useful FAST images, and to determine if an 8th grade student can teach peers these skills.@*METHODS@# The 8th grade students at a small middle school were divided into two groups. One group received training by a certified medical sonographer, while the other group received training by a peer 8th grade student trainer who had previously received training by the sonographer. After training, each student was independently tested by scanning the four FAST locations. A blinded ultrasound expert evaluated these images and deemed each image adequate or inadequate for clinical use.@*RESULTS@# Eighty video image clips were obtained. The overall image adequacy rate was 74%. The splenorenal window had the highest rate at 95%, followed by retrovesical at 90%, hepatorenal at 75%, and subxiphoid cardiac at 35%. The adequacy rate of the sonographer-trained group was 78%, while the adequacy rate of the student-trained group was 70%. The difference in image adequacy rate between the two groups was not significant (P-value 0.459).@*CONCLUSION@# The majority of 8th graders obtained clinically adequate FAST images after minimal training. Additionally, the student-trained group performed as well as the sonographer-trained group.
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Objective To analyze the blind area monitoring and independent image acquisition function of gastroscopic elves ( a real-time gastroscopic monitoring system) in gastroscopy. Methods A total of 38522 gastroscopic images from the database of Digestive Endoscopy Center of Renmin Hospital of Wuhan University were collected to train and validate the gastroscopic elves. Using computer to generate random numbers, 91 gastroscopic videos were selected to assess the position recognition accuracy of the gastroscopic elves, and 45 gastroscopic videos and matching gastroscopic images collected by endoscopists were selected to compare the coverage number and rate of gastroscopy sites between gastroscopic elves and endoscopists image acquisition. Two endoscopists entered the study to perform gastroscopies with or without gastroscopic elves. Forty-five gastroscopies respectively performed by the endoscopist A before and after usage of gastroscopic elves were collected, and 42 gastroscopies divided into 20 and 22 performed by the endoscopist B without use of gastroscopic elves in the same period were also collected. The coverage rate of gastroscopy sites was compared between the two endoscopists. Results The total position recognition accuracy of gastroscopic elves was 85. 125％ ( 1156/1358) . The coverage rate of gastroscopic sites for the endoscopist A was (76. 790±8. 848)％ and (87. 325±7. 065)％, respectively, before and after using gastroscopic elves, and the coverage rate in the same period for the endoscopist B was (75. 926 ±11. 565)％ and (75. 253 ± 14. 662)％, respectively. The coverage rate before using gastroscopic elves had no statistical difference between the two endoscopists (t=0. 324, P=0. 747). The coverage rate for the endoscopist A after using gastroscopic elves was higher than that before using gastroscopic elves ( t=6. 222, P=0. 001) , and that of the endoscopist B in the same period ( t'=3. 588, P=0. 002) . The coverage number and rate of gastroscopy sites for gastroscopic elves and endoscopists image acquisition were 20. 956 ± 3. 406 and ( 77. 613 ± 12. 613)％, and 15. 467 ± 2. 296 and ( 57. 284 ± 8. 503)％, respectively, with statistical differences ( t=11. 523, P=0. 000; t=11. 523, P=0. 000). Conclusion Gastroscopic elves can improve the coverage number and rate of gastroscopy sites, and is worthy of promotion in clinics.
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Objective To analyze the application status and problems of PACS so as to facilitate its future development.Methods The application of PACS in some hospital from June 2015 to May 2016was reviewed,and considerations were taken on the hospital conditions to explore the application status and problems of PACS in China.Results There were a number of problems found in Department of International Diagnosis and Treatment,including 13-a active time of image acquisition devices,6.8％ of the workstations and 14.2％ of the image acquisition devices having no interface with PACS,and 2.9％ of the images non-uploaded to PACS.Conclusion PACS in China has to emphasize on stability,high efficiency,safety and reliability to facilitate its future application to medicine,scientific research and teaching.
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Objective To analyze the application status and problems of PACS so as to facilitate its future development.Methods The application of PACS in some hospital from June 2015 to May 2016was reviewed,and considerations were taken on the hospital conditions to explore the application status and problems of PACS in China.Results There were a number of problems found in Department of International Diagnosis and Treatment,including 13-a active time of image acquisition devices,6.8％ of the workstations and 14.2％ of the image acquisition devices having no interface with PACS,and 2.9％ of the images non-uploaded to PACS.Conclusion PACS in China has to emphasize on stability,high efficiency,safety and reliability to facilitate its future application to medicine,scientific research and teaching.
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Objective To design an endoscopic image acquisition system.Methods High resolution CCD image sensor was used as the image acquisition equipment to converse the optical signals into electrical ones. Data were stored with FPGA controller after ADC, and then transmitted to the computer with PCI bus,Results Trials proved that the system could acquire clear and stable image with simple structure and easy operation.Conclusion The system can acquire high-quality images for the diagnosis and treatment of endoscope.
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OBJECTIVES: Aluminum step wedge (ASW) equivalent radiodensity (eRD) has been used to quantify restorative material's radiodensity. The aim of this study was to evaluate the effects of image acquisition control (IAC) of a digital X-ray system on the radiodensity quantification under different exposure time settings. MATERIALS AND METHODS: Three 1-mm thick restorative material samples with various opacities were prepared. Samples were radiographed alongside an ASW using one of three digital radiographic modes (linear mapping (L), nonlinear mapping (N), and nonlinear mapping and automatic exposure control activated (E)) under 3 exposure time settings (underexposure, normal-exposure, and overexposure). The ASW eRD of restorative materials, attenuation coefficients and contrasts of ASW, and the correlation coefficient of linear relationship between logarithms of gray-scale value and thicknesses of ASW were compared under 9 conditions. RESULTS: The ASW eRD measurements of restorative materials by three digital radiographic modes were statistically different (p = 0.049) but clinically similar. The relationship between logarithms of background corrected grey scale value and thickness of ASW was highly linear but attenuation coefficients and contrasts varied significantly among 3 radiographic modes. Varying exposure times did not affect ASW eRD significantly. CONCLUSIONS: Even though different digital radiographic modes induced large variation on attenuation of coefficient and contrast of ASW, E mode improved diagnostic quality of the image significantly under the under-exposure condition by improving contrasts, while maintaining ASW eRDs of restorative materials similar. Under the condition of this study, underexposure time may be acceptable clinically with digital X-ray system using automatic gain control that reduces radiation exposure for patient.
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Humans , Aluminum , Radiographic Image EnhancementABSTRACT
Objective To explore the mechanical testing technique suitable for biological materials under water environment. Methods Based on digital image correlation (DIC) method, the unique lens sleeves which can avoid the distortion caused by underwater photography was designed, and this technique was applied to determining mechanical properties of the fish scales. Results The experiment on translation of the water sink indicated that the use of the designed lens sleeves could effectively reduce errors for underwater measurement with high precision; the mechanical testing on fish scales showed that different regions of the fish scales had obviously different mechanical properties, but the differences induced by regions of the dehydrated fish scales were significantly reduced. Conclusions The designed lens sleeves in this study can be applied to image acquisition effectively, and determination of mechanical properties of the biological materials under water environment was achieved using DIC method.
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Objective To develop a graphic reporting system,which should have good scalability,and be able to connect with the hospital information system,to restore the operation of colposcopy.Methods The system adopted multi-layer C/S structure.Microsoft SQL Server database platform was used in data layer.Application service layer and client application were developed with Delphi.ADO components were integrated into application service layer to connect the database.Client applications and application service layers communicated with each other through the SOCKET protocol.Image processing module was based on the acquisition hardware design,using the development kits provided by the hardware merchant to achieve acquisition and processing functions.Results The graphic reporting system achieved real-time image acquisition,and possessed the function of flexibly generating reports.Besides dynamic setting of the menu,the system was even innovative in achieving the report's custom settings,which could be easily carried out in different departments because of its capability of setting up totally different kinds of report contents and output format according to different demands.Conclusion The graphic reporting system meets the design requirements for restoring the operation of the original colposcopy equipment.Furthermore,it is innovative among the similar systems in achieving a dynamic interface design,which has a good scalability to meet the demand for similar departments.It resolves the problem of developing the hardware-based personalized image acquisition software,so that it can effectively reduce the operational costs.
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This paper introduces a multi-function DSA image workstation, the main structure is described. The structure of software system are emphasized on. The image workstation has a variety of functions, such as image acquisition, data transferring, archiving and processing. The client/server software modules is used for digital image processing and communication in medicine.