ABSTRACT
Objective To explore the clinical value of related indicators of blood analysis in the differential diagnosis of acute and chronic leukemia .Methods From January 2015 to June 2017 ,15 patients with acute leukemia ( A group ) and 10 patients with chronic leukemia ( B group ) , and 20 healthy volunteers ( C group ) , 20 cases of nosocomial infection(D group) in Jiaocheng People's Hospital of Lyuliang were selected .The differences of WBC, RBC,PLT,HB in four groups were analyzed,and the differences of WBC,immature granulocyte percentage (IG％), immature granulocyte(IG),the percentage of naive cells ,immature cells count between A group and B group were further analyzed.Results The count of WBC from high to low was B group [(120.3 ±39.2) ×109/L],A group [(81.4 ±29.2) ×109/L],D group[(26.3 ±10.3) ×109/L],C group [(5.4 ±1.9) ×109/L],there was statistically significant difference among the four groups (P <0.05).The count of RBC in C group [(4.6 ± 1.3) ×109/L] ≈D group [(4.0 ±0.9) ×109/L] >B group [(1.4 ±0.7) ×109/L] >A group [(0.6 ± 0.1) ×109/L](P<0.05).The count of PLT in D group [(361.5 ±103.3) ×109/L] >C group [(264.3 ± 84.2) ×109/L] >B group [(12.3 ±6.5) ×109/L] ≈A group [(5.9 ±1.7) ×109/L](P<0.05).Through the analysis of bone marrow AML 11 cases,54.55％was M2,followed by M1,M3,M5.ALL in 4 cases,CML in 10 cases. The IG％,IG count,WBC,percentage of naive cells ,immature cells count in A group and B group were increased ,but in the different types of leukemia increased in amplitude had significant difference , AML was characterized by the increase of IG％and percentage of naive cells ,ALL was characterized by the significant increase of WBC ,CML was characterized by the significant increase of WBC ,IG％,IG count.Conclusion Blood analysis is of high value in the differential diagnosis of acute and chronic leukemia .It can provide a reliable basis for the initial diagnosis and classification of leukemia ,and can be used to guide clinical treatment .
ABSTRACT
INTRODUCTION: The ABX Pentra DX 120 (Pentra DX 120, ABX Diagnostics, Montpellier, France) adopted new technologies to perform differential leukocyte and erythroblast counts. The double matrix can discriminate Large Immature Cell (LIC), Immature Granulocyte (IMG), Immature Monocyte (IMM), Immature Lymphocyte (IML), and Atypical lymphocyte (ALY) in addition to a routine 5-differential count. For erythroblast (ERB), a fluorescence method is employed. In this study, we evaluated the performance of the Pentra DX 120 in the performance of differential leukocyte and erythroblast counts. METHODS: Precision was evaluated using 3-level control materials. Comparison analysis was performed on 200 samples: 100 normal and 100 abnormal samples. We evaluated the 5 part differential count, LIC, IMG, IMM, IML, ALY, and ERB. These parameters were analyzed in comparison with the results from the reference method, manual differential count. RESULTS: The coefficients of variation (CVs) of precision were 0.9 except monocytes and basophils. IMG, ALY and erythroblasts were also well correlated with manual count (r=0.8315, 0.5602, 0.8144, respectively). The efficiency of flagging system was 84% for LIC, 80% for ALY, and 78.0% for increased ERB (>2/100WBCs). CONCLUSIONS: The Pentra DX 120 performed reliable differential leukocyte and IMG, ALY, and ERB results demonstrated comparable performance to manual count. And, the flagging system was efficient for detecting each abnormal cell population. We expect the Pentra DX 120 double matrix and erythroblast count can reduce microscopic review rate in routine laboratory and promote laboratory efficiency.