ABSTRACT
Objective:To investigate the protective effect of ginkgetin from oxidative stress on immature testis induced by cyclophosphamide(CP) metabolite acrolein(ACR) in vitro.Methods:The sertoli cells were isolated from 7-days old SD rats' testes,culture and passage to F2 generations.Experiment groups were divided into: 1)Ginkgetin incubated for 12 hours and then treat with ACR 2) Ginkgetin and ACR add to cells at the same time 3) Ginkgetin and ACR were incubated for 12 hours,then add to cells.Control group include: 1) Vitamin E were incubated cells for 12 hours,then add ACR respectively 2) Added ACR only as the negative control group 3) Not add any factors as the positive control group.3 h later the viability of sertoli cell were determined by MTT.Protein concentration was determinate by Coomassie Brilliant Blue.Malondialdehyde(MDA),hydroxyl radical(OH ?),total antioxidant capacity(T-ACO),Superoxide dismutase(SOD) and glutathione reductase(GR) activity were tested.Results:1) Ginkgetin(add before ACR) can significantly raise the viability of the cells(P