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Objective@#To investigate the change in expression of anti-senescence marker protein calmodulin (RGN) in liver tissues of rats with immune hepatic fibrosis, and to observe the effect of compound glutathione inosine injection (CGII) on it.@*Methods@#Rat liver fibrosis model was induced by intraperitoneal injection of porcine serum, and CGII intervention was administered at the appropriate time. Rat liver tissues were stained with HE and Masson. RGN and protein expression at mRNA in liver tissues was detected by fluorescence quantitative PCR and immunohistochemistry. One-way Anova was used for measurement data. LDS test was used for two-way comparison, and pathological semi-quantitative results were analyzed by rank-sum test.@*Results@#The relative expression of RGN mRNA and protein in liver tissue of fibrotic rats was 82.23 ± 15.21 and 12.52 ± 3.23, respectively, which were significantly lower than that of normal rats 176.39 ± 11.35 and 59.23 ± 9.13 (P < 0.01). The degree of liver fibrosis in fibrotic rats after CGII intervention was significantly lower than fibrotic rats. The relative expression of RGN mRNA and protein in the intervention group was 168.78 ± 21.31 and 46.42 ± 4.71, respectively, which were significantly higher than fibrosis and spontaneous recovery group. The difference was statistically significant (P < 0.01). The relative expression of RGN mRNA and protein in the spontaneous recovery group was 86.23 ± 17.16 and 14.34 ± 5.16, which was higher than model group. The difference was not statistically significant (P > 0.05).@*Conclusion@#The expression of RGN in liver tissue of rats with hepatic fibrosis induced by porcine serum is decreased, while the expression of RGN increases with the decrease of fibrosis after CGII intervention, suggesting that the protein may play an important role in the development of liver fibrosis.
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OBJECTIVE: To research the protective effects of Acanthus ilicifolius alkaloids A and its two derivatives in the rats with immune hepatic fibrosis. METHODS: Immune hepatic fibrosis model was established by intraperitoneal administration porcine serum. SD rats were treated intragastric administration with [4-hydroxy-2 (3H)-benzoxazolone, HBOA], [4-acetoxy-2 (3H)-benzox-azolone, AcO-BOA], (3-acetyl-4-acetoxy-2-benzoxazolone, TC-3). The activities of ALT, AST in serum and the levels of HA and LN in serum were measured. RESULTS: Compared with the normal control group, the activities of ALT, AST and the levels of HA and LN in the model group were significantly increased. Compared with the model group, animals treated with the HBOA, AcO-BOA or TC-3 could decrease the activities of ALT and AST and the levels of HA and LN markedly. Histopathological results showed that treatment groups could relieve the porcine serum-induced liver damage. CONCLUSION: HBOA and its derivatives have protective effect on the rats with immune-mediated hepatic fibrosis.
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Objective To research the effects of Xiongshao Decoction (XSD) on immune liver fibrosis in rats. Methods Eighty-four SD rats were randomly divided into normal control group, model group, Fuzheng Huayu (FZHY) Capsule group, XSD prevention group, XSD high-dose and low-dose groups (15.8 and 7.9 g/kg). The hepatic fibrosis model was induced by injecting porcine serum to the abdominal cavity of rats. To observe the effects of XSD on the serum hepatic function of rats: ALT, AST, ALB, and AJG, the serum marker of hepatic fibrosis: hyaluronic acid (HA), laminin (LN), procollagen III (PCIII), and collogen IV (CIV), and hepatic tissue pathomorphology as well. Results Compared with the model group, the levels of ALT, AST, HA, LN, and PCIII in every XSD treatment group were obviously decreased (P<0.05, 0.01), the levels of ALB and A/G were obviously increased (PO.01). The CIV level of serum in XSD prevention group was significantly decreased (P<0.05) and the degrees of hepatocyte degeneration and necrosis in all treatment groups were lower, with less liver fibrosis, especially those in XSD prevention group and high-dose group. Conclusion XSD could not only relieve the degree of liver cells damage of immune hepatic fibrosis in rats, improve hepatic function of them, and regulate the extracellular matrix metabolism, but also arrest the hepatic fibrosis or make it reversed.
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Objective To explore the fluctuation of cytokine mRNA expression level in a novel T-cell-mediated immune hepatic fibrosis model induced by repeatedly injections of Concanavalin A in BALB/c mice. Methods BALB/c mice were divided into different groups. Model group mice were injected weekly up to 20 weeks with Concanavalin A (15mg/kg), via retro-orbital venous plexus under ether anesthesia. Normal control group mice were treated in the same manner weekly with normal saline. Twenty-four hours after Concanavalin A challenge at 1, 5, 12 and 20 week, 8 mice from each time were killed by cervical dislocation, repectively. The livers of different group were excised and fixed in 10% formalin for HE staining and Gomori Ag staining or frozen in optimal cutting temperature (O.C.T.) media in liquid nitrogen for immunohistochemical staining for CD4 +T or CD8 +T cell. After extracting total RNA from liver tissues, IL-2, IL-4, IL-10 and transforming factor ?1 messenger RNA were amplified by reverse transcription polymerase chain reaction. PCR products were electrophoresed on agrose containing ethidium bromide and visualized under ultraviolet light. Densitometric RT-PCR data were standardized with ?-actin signals. Results The histological change of HE staining and Gomori Ag staining indicated the fibrogenesis in model group mice. Immunohistochemical staining for CD4 + or CD8 + T cell indicated that the infiltrating lymphocytes in liver parenchyma were mainly CD4 +T lymphocytes. IL-2 mRNA expression level only increased after the first injection of Concanavalin A. The expression levels of IL-4, IL-10 and transforming growth factor ?1 mRNA significantly increased over the whole experiment period as compared with control group. Conclusions Repeated administration of Concanavalin A can induce T-cell-mediated immune hepatic fibrosis model in BALB/c mice. The expression levels of IL-4, 10 and TGF-?1 increase over the whole experiment period and may play an important role in creating mouse fibrotic model.