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The immune system as an important defense system of the body, bear the resistance to foreign pathogens invasion, removal of foreign heterogeneity, and protect the body's safety. Modern research shows that tonic Chinese medicine, including single herband compound formula, has the function of improving immune organ index, enhancing immune cellfunction and affecting the immune molecule production and secretion. This article will review the effects of traditional Chinese medicine on immune organs, immune cells and immune molecules, and provide reference for the clinical research of traditional Chinese medicine.
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Objective To investigate the allergens and study the clinical significance of detecting serum levels of TNF-α,IL-4, IL-6 and IL-8 in patients with bronchial asthma.Methods 62 cases of patients with bronchial asthma were selected from August 2011 to August 2013 in Baoji Central Hospital,enzyme-linked immunosorbent assay were carried out in patients u-sing standardized allergens.62 cases of healthy people were selected as control group,and detected serum levels of TNF-α, IL-4,IL-6 and IL-8 of the two groups.Results Dust mites were the most important and the strongest allergens.The levels of TNF-α,IL-4,IL-6 and IL-8 in observation group was significantly higher than those in control group (230.87±27.82 ng/ml vs 152.14± 23.27 ng/ml;25.32 ± 12.04 ng/L vs 8.57 ± 3.64 ng/L;152.45 ± 24.56 μg/L vs 98.56 ± 18.63 μg/L;218.79±32.91μg/L vs 142.68±23.76μg/L,P<0.05).Conclusion TNF-α,IL-4,IL-6 and IL-8 levels in patients with bronchial asthma j udgment can be used as an important predictor,and it is worthy of clinical application.
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Objective To observe the protective effect of the zinc gluconate combined with Salvia injection on noise induced serum immunoglobulin(IgG,IgM,IgA) and immune molecules (IL-2,IL-4) in rats.Methods 50 female SD rats were randomly divided into control group,noise group,zinc gluconate noise group(plus zinc group),Salvia injection noise group (plus Salvia group) and Zinc gluconate oral liquid and Salvia injection noise group(combined group),10 rats in each group.Except the control group,rats in the other groups were continuously exposed to high frequency steady noise for two weeks.The concentrations of serum immunoglobulin (IgG,IgM,IgA) and immune molecules (IL-2,IL-4) after the intervention of noise were compared.Results Serum levels of IgG,IgM,IL-2,IL-4of the control group were significantly higher than the noise group (all P ＜ 0.05),plus zinc group (all P ＜ 0.05),plus Salvia group(all P ＜0.05),the differences were statistically significant.Serum level of IgA of each group was not statistically different compared with control group (all P ＞ 0.05).Serum levels of IgG,IgM,IL-2,IL-4 in the noise group were significantly lower than those in the control group (all P ＜ 0.05),combined group (all P ＜ 0.05),the differences were statistically significant.Serum levels of IgG,IgM,IL-2 in plus zinc group were significantly higher than the noise group(all P ＜ 0.05).Serum IgA concentration of each group showed no significant difference compared with noise group(all P ＞ 0.05).Conclusion Zinc gluconate oral solution and Salvia injection have protective effect on immune system affected by noise in rats,the protective effect of combination of zinc gluconate oral solution and Salvia injection is stronger than the individual.
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Objective To investigate the effect of hepatitis B virus X (HBX) gene on apoptosis and immune molecules of human proximal renal tubular epithelial cell line (HK-2).Methods The eukaryotic vector pcDNA3.1-myc-HBX containing HBX gene was transiently transfected into HK-2 cells by lipofectamine mediation.Untransfected HK-2 cells and those transfected with empty vector were used as controls.The TLR4 expression was detected by real-time PCR and Western blotting.The apoptosis of cells and expression of MHC-Ⅱ and CD40 were detected by flow cytometry,and the contents of IL-4 and IFN-γ in the supernatant were detected by EIISA.Results Compared with control groups,the number of apoptotic cells was significantly increased in the HBX transfection group (P ＜ 0.05),and the expressions of TLR4,MHC-Ⅱ and CD40 were also significantly increased in the HBX transfection group (all P＜0.05).IFN-γ level in the supernatant of HBX transfection group was higher (P ＜ 0.05),but IL-4 level was lower as compared to control groups (all P ＜ 0.05).Conclusions Over-expression of HBX gene may induce apoptosis of HK-2 cells and upregulate the expression of immune molecules of renal tubular epithelial cells leading to injury of cells and dysfunction of immunomicroenviroment.
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@#Neurotrophins promote and modulate the repair and regeneration of central nervous system (CNS) on the levels of synapses, neurites and neural cells, and even of the auxiliary structures of CNS. As to immune molecules, recent studies denied the classical doctrine that CNS is an immune privilege organ. In the last decade, it is found that immune responses can be beneficial for CNS repair. What are more, some macromolecules that were previously thought to be the members of the family of immune system play essential roles in the development and regeneration of the CNS. Therefore, the authors postulate that there are crosstalks between the neurotrophins and the immune molecules in the CNS.