ABSTRACT
Objective@#To investigate the cellular and humoral immune responses induced by combined immunization with the fusion protein of human papillomavirus type 18 (HPV18) and the recombinant vaccinia virus.@*Methods@#Purified HPV18L231-600E7E6 fusion protein, expressed by prokaryotic expression system, were immunized in combination with the recombinant vaccinia virus vaccine expressing HPV18E7E6 fusion protein (rVV18E7E6) by using various prime-boost regiments in C57BL/6 mice. Cellular and humoral immune responses were analyzed by enzyme-linked immunospot assay (ELISPOT), enzyme-linked immunosorbent assay (ELISA), and pseudovirus neutralization assay.@*Results@#Higher levels of cellular immune responses were induced in mice primed with the HPV18L231-600E7E6 fusion protein/adjuvant CpG and boosted with the recombinant vaccinia virus rVV18E7E6, than in other immunized mice. Higher binding antibody level was induced, and low level neutralizing antibody against pseudovirus was detected simultaneously.@*Conclusions@#Priming with HPV18L231-600E7E6 fusion protein/CpG and boosting with the recombinant vaccinia virus rVV18E7E6 could induce higher cellular and humoral immune response in immunized mice, which might be taken as vaccine candidate for treatment of HPV18 chronic infection and postoperative adjuvant treatment for cervical cancer.
ABSTRACT
The incidence of cervical cancer is the first place in gynecological malignant tumors in China. About 500,000 new cases accounts for 5% of all new cancer patients appeared per year all over the world.How to prolong the progression free survival and overall survival of patients with cervical carcinoma is a very important issue in the field of gynecology.So it is very important to take effective treatment.This paper reviewed the research progress of cervical carcinoma treatment in recent years,including surgical treatment,chemotherapy drugs thera?py,angiogenesis inhibitors targeted therapy,immunization therapy.
ABSTRACT
There are some tre at ments described in this paper for neonates hypoxic-ischemic encephalopathy (HIE ), such as, inhibitors of the ion passway of calcium, blockers of the excitation amino acid, the body remover of the free radicals of oxygen, immunization thera py, complex compound of iron, and inhibitor of NO enzyme.