ABSTRACT
Objective To observe the association of vesicular glutamate transporter of typeⅠ (VGluT1 ) like immunoreactive(LI) ,the differentiation-associated Na+ dependent inorganic phosphate cotransporter(DNPI) LI and glutami cacid decarboxy lase(GAD) LI terminals with GABAA receptor ?3 subunit(GABAAR ?3) LI neurons in mesencephalic trige minal nucleus of the rat.Methods Triple immun of luorescencehis to chemical staining technique and confo call aser scanning micros copy were used.Results Alargenumber of neuronal cell bodies showed GABAA R?3 LI immunoreactivity atallrostrocaudal level softhe Vme ,and most of GABAAR ?3 LI cells were large (2 5 5 0 ?m)pseudouni polarneurons.The dense VGluT1 LI,DNPL LI and GAD LIterminal sdistri buted widelyin Vme ,some VGlu T1 DNPI LI and GAD LIterminals surrounded the somata of the GABAAR ?3 LI Vmeneurons ,and made close contacts with them .Conclusion Proprioceptive sensory signals from the or of acialregionmight be modulated at the level of the primary afferent cell bodies in the Vme both by glutamatergic and GABAergic axonal terminals from other brain areas,and the effect of GABAergic terminals might be mediated by post synaptical GABAA receptors .
ABSTRACT
Objective To observe the connections between the glutamic acid decarboxylase\|like immunoreactive(GAD\|LI) axonal terminals and the positive neurons showing phosphate actived glutaminase(PAG)\|LI or GABA\-A\|receptor ?3 subunit (GABA\-AR?3)\|LI and parvalbumin(PV)\|LI in the mesencephalic trigeminal nucleus(Vme) of the rat. Methods The triple\|immunofluoresence histochemical staining techniques were used in the present study,and stained sections were observed under a confocal laser\|scanning microscope. Results Many neuronal cell bodies through the whole rostrocaudal extent of Vme were showed dense GABA\-AR?3\|,PAG\| and PV\|like immunoreactivities,respectively.The majority of them were large pseudounipolar neurons(diameter 25\|50?m).About 90% of PAG\|LI neurons expressed both GABA\-AR?3\|LI and PV\|LI.Confocal laser\|scanning microscope further revealed that dense GAD\|LI axonal varicosities were found to surround the somata of Vme neurons showing both GABA\-AR?3\| and PAG\|like immunoreactivities,and formed the close contact each other.Conclusion\ The present results suggested that the GABAergic axonal terminals projecting to Vme might exert inhibitory regulation and control effect through GABA\-AR?3 subunit localizated in somata of Vme neurons on the transmission of orofacial region proprioceptive sensory information mediated by glutamate.