ABSTRACT
Objectives:To investigate the relationship between calcitonin gene-related peptide(CGRP) in rat dental pulp and tooth movement.Methods:The first maxillary molars in 36 SD rats were moved mesially by orthodontic force,18 were examined 3,7 and 14 day after application of the appliance whereas another 18 rats were observed 7,14 and 28 days after removal of the appliance respectively.6 control rats were without treatment.Then,CGRP immunoreactivity was demonstrated by indirect immunoflurescence on frozen sections of dental pulp samples.Results:3,7 and 14 days after application of orthodontic force, the CGRP containing nurve fiber counts in each pulp were 17.57?4.42,24.04?3.55 and 21?4.11 respectively,those in the control pulps were 8.03?4.49. 7,14 and 28 days after removal of the appliance, the counts were 19.23?5.23,18.23?5.08 and 8.12?5.01 respectively.Conclusions:CGRP immunoreactive nerve fibres may take an active part in tissue responses in pulp tissues during experiment tooth movement.
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Objective To evaluate the sensitivity and speciality of protein chip technology, and discuss its value in diagnosis or classification of autoimmune diseases, and to make its methodological evaluation. Methods The anti-dsDNA was detected with gold-colloid assay, indirect immunoflurescence(IIF) assay and protein chip technology, respectively; the other seven autoantibodies including anti-SSA, anti-SSB, anti-Sm, anti-u1RNP, anti-Rib-P, anti-Scl-70 and anti-Jo-1 were simultaneously detected with immunoblotting(IBT) assay and protein chip technology, and then all the results were delt with statistical method. Results For anti-dsDNA, the sensitivity of protein chip technology was better than that of gold-colloid assay; there was significant difference between protein chip technology and IBT assay in detecting anti-Jo-1 in DM/PM(P
ABSTRACT
The distribution of microtubules (MTs)in the Sertoli cell of rat testis was studied with indirect immunofluorescence and electron microscopy. We have found that MTs are mainly located in the cytoplasm apical to the nucleus and oriented parallel to the long axis of the Sertoli cell. MTs may extend into the stalks and processes of the cell which embrace different germ cells. With the changes of the architecture of the seminiferous epithelium and the shape of Sertoli cells, there are some regular changes in MTs distribution during the seminifeous epithelium cycle. In stage Ⅰ-Ⅴ, many MTs aggregate around the elongated spermatids and are parallel to their long axis. During stage Ⅶ maturing spermatids are suspended into the lumen by the Sertoli cell processes containing numerous MTs. Some of the MTs conform to the contour of the hook-shaped spermatids heads. After spermiation (stage Ⅶ-Ⅸ), MTs retract from the lumen with the Sertoli cell processes and gather around the spermatids which just start their elongation. These results indicate that the distribution of the MTs in Sertoli cell has close relations with the architecture of the seminiferous epithelium, and the changes of the Sertoli cell shape and the movement of the spermatids.