ABSTRACT
Since the first session of Chinese anti-inflammatory immunopharmacological society in 1982,many breakthroughs of immunological research have been made in western countries,such as identification of pathogens by natural immune cells,regulation of immune response,lymphocyte differ?entiation and development. At the same time,much progress has been achieved in anti-inflammatory and immunological research in China,especially in the field of basic scientific issues in immunology, frontier and hot topics,immunological mechanisms of major diseases and related drugs. The course of development of Chinese anti- inflammatory immunopharmacological research has experiences various stages,such as ″follow ″ in the past to ″work together ″ and to ″lead ″ in some research areas in today′s international immunology.
ABSTRACT
Objetivo: para determinar la utilidad de herramientas inmunoinformáticas para detectar péptidos que puedan ser inmunodominantes, y evaluar las diferencias entre las respuestas inmunes de los modelos animales empleados en los estudios preclínicos y en los humanos. Métodos: se modeló la respuesta frente a dos proteínas exógenas: la estreptocinasa recombinante y el antígeno de superficie de la hepatitis B. A partir de sus secuencias primarias se emplearon algoritmos para identificar epítopes B y T frente a moléculas HLA de clase I y II (HLA-A*0201, HLA-DRB1*0301 y HLA-DRB1*0701) y los haplotipos murinos H2-Kd y H2-Kk. Se seleccionaron los péptidos de más alta puntuación. Resultados: el algoritmo ABCPred mostró una mejor capacidad de predicción de epítopes B, mientras fue mayor la coincidencia para los programas de modelación de la respuesta T. Los epítopes generados para el haplotipo H2-Kk tuvieron una similitud mayor con los presentados por las moléculas HLA seleccionadas. Conclusiones: se presenta una metodología aplicable al desarrollo de vacunas de subunidades y multiepitópicas, así como para otros fármacos biotecnológicos de naturaleza peptídica, que permite optimizar las etapas preclínicas y clínicas, a muy bajo costo, mínimos requerimientos tecnológicos, utilización óptima de medios, recursos y capital humano disponibles en cualquier institución del sistema nacional de salud
Objective: determine the usefulness of immunoinformatics tools to detect potentially immunodominant peptides, and evaluate the differences between the immune responses provided by the animal models used in preclinical and human studies. Methods: modeling was conducted of the response to two exogenous proteins: recombinant streptokinase and hepatitis B surface antigen. Based on their primary sequences, algorithms were used to identify B and T epitopes against HLA class I and II molecules (HLA-A*0201, HLA-DRB1*0301 and HLA-DRB1*0701), and murine haplotypes H2-Kd and H2-Kk. The highest scoring peptides were chosen. Results: ABCPred algorithm showed a better prediction capacity for B epitopes, whereas coincidence was greater in modeling programs for the T response. The epitopes generated for haplotype H2-Kk had greater similitude with those presented by the HLA molecules selected. Conclusions: a methodology is presented which is applicable to the development of subunit and multiepitope vaccines, as well as other peptidic biotechnological drugs. This methodology allows optimization of the preclinical and clinical phases at a very low cost, with minimal technological requirements, optimal use of media, and resources and human capital available at any institution of the national health system
Subject(s)
Humans , Hepatitis B Antigens/analysis , Recombinant Proteins/immunology , Vaccines, Subunit/immunologyABSTRACT
AIM: To investigate the effect of cycloheximide on the T cells activation by mitogen in vitro with CD69 expression as activation marker for the application of this drug clinically. METHODS:Lymphocytes were isolated from lymphoid nodes of C57BL/6 mouse. The cells were preincubated with cycloheximide(CHX), 5% serum containing CHX respectively for an hour, then further incubated with polyclonal activators (Con A or PDB). Harvesting the cells after whole incubation for 24 h, we estimated the expression rates of CD69 on T cells by flow cytometry following two-color immunofluorescent staining. RESULTS: The expression rates of CD69 on the T cells preincubated with CHX, serum containing CHX after the stimulation in response to Con A or PDB all showed significant difference with the expression rates of control group, respectively ( P
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The main fields and trends of research on anti-inflammation and immunopharmacology were reviewed as: signal transduction pathways as target for therapy, cytokine regulating network, new types of immunotherapy, new mechanisms of anti-inflammation drugs, the mechanisms and inductive methods of immune tolerance and the development of natural immune system.
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In this report, the immunopharmacological effects of Polyactin A ( PAA ) were presented. When the SRBC immunized mice were administered daily 2. 5~40mg/kg ( ip ) of PAA for 3 or 5 days, the serum level of hemolysin, the antibody production in spleen and the number of nucleated cells of spleen were enhanced significantly; and the efficacy-dose dependency was evident in these experiments。 In spite of these effects, PAA didn't affect the kinetics of circulating antibody level.In other experiments, it was found that the efficiency was more potent when PAA was given before or simultaneously with SRBC immunization than given after.