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【Objective】 To optimize the existing spin-EB method and promote human induced pluripotent stem cells (hiPSCs) differentiate into megakaryocytes (MKs). 【Methods】 In this study, the initial inoculation amount of hiPSCs was increased from 3 500 cells/well to 8 000 cells/well, and the size of EB was increased. By observing the generation time of EB- hematopoietic cells during differentiation, and detecting the proliferation of CD34+ hematopoietic progenitor cells and CD41+ MKs in different stages, it was studied whether the optimized scheme could promote the differentiation of hiPSCs into hematopoietic progenitor cells(HPCs) and MKs. 【Results】 By increasing the initial inoculation amount of hiPSCs and the size of EB, the differentiation of hiPSCs into HPCs and MKs and the cell production efficiency can be promoted. 【Conclusion】 Our research describes an optimized and repeatable differentiation method, which can produce hematopoietic progenitor cells and mature MKs from hiPSCs in a relatively short time with higher yield. It is of great clinical significance and broad scientific research prospect to continuously optimize the culture scheme of hiPSCs differentiation to produce MKs and platelets in vitro, and to promote large-scale platelet generation in vitro in transfusion medicine.
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Gastrointestinal polyps are treated as the precursors of cancer development. So, possibility of cancers can be reduced at a great extent by early detection and removal of polyps. The most used diagnostic modality for gastrointestinal polyps is video endoscopy. But, as an operator dependant procedure, several human factors can lead to miss detection of polyps. In this peper, an improved computer aided polyp detection method has been proposed. Proposed improved method can reduce polyp miss detection rate and assists doctors in finding the most important regions to pay attention. Color wavelet features and convolutional neural network features are extracted from endoscopic images, which are used for training a support vector machine. Then a target endoscopic image will be given to the classifier as input in order to find whether it contains any polyp or not. If polyp is found, it will be marked automatically. Experiment shows that, color wavelet features and convolutional neural network features together construct a highly representative of endoscopic polyp images. Evaluations on standard public databases show that, proposed system outperforms state-of-the-art methods, gaining accuracy of 98.34%, sensitivity of 98.67% and specificity of 98.23%. In this paper, the strength of color wavelet features and power of convolutional neural network features are combined. Fusion of these two methodology and use of support vector machine results in an improved method for gastrointestinal polyp detection. An analysis of ROC reveals that, proposed method can be used for polyp detection purposes with greater accuracy than state-of-the-art methods.
Subject(s)
Humans , Endoscopy , Methods , Polyps , Sensitivity and Specificity , Support Vector MachineABSTRACT
Objective To introduce an improved method for collecting the blood of rat by cardiac puncture .Methods The study se-lected 90 Wistar rats,half male and half female ,and then the rats were anesthetized by intraperitoneally injecting 10％chloral hydrate solution according to 0.3 mL/kg body weight.Then using three-line positioning method to determine the puncture site ,the blood collection needle and blood collection tube were used to collecting the blood ,Finally,the blood volume of each rat was recorded .Results The success rate was cal-culated according to the single blood collection of 5 mL or more,and our 90 rats were collected 90 times,the success rate was about 82.2％. After centrifugation,the total serum was about 260 mL,the average serum separation of each rat was about 2.8 mL.Conclusion The im-proved positioning method of heart blood collection is intuitive and easy to learn ,and the total volume and quality of blood is high ,besides ,the new method has a small surgical trauma and a high efficiency ,which is worth to extend .
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OBJECTIVE:To improve the content determination for polygala xanthone Ⅲ in Polygalae Radix contained in Chi-nese Pharmacopoeia (2010 edition). METHODS:HPLC was performed on the column of Hypersil BDS C18 with mobile phase of acetonitrile-0.05% phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min;detection wavelength was 320 nm,column tem-perature was 25℃,and the injection volume was 10μl. RESULTS:The linear range of polygala xanthoneⅢwas 0.029-0.928 μg/ml (r=0.999 1);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 94.66%-100.90%(RSD=2.46%,n=6). CONCLUSIONS:The method is simple,stable and reproducible. Although the determination time is prolonged,it has improved the accuracy and it is more suitable for the content determination of polygala xanthoneⅢin Polygalae Radix.
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To understand the continuing medical education status of Beijing Shijitan Hospital,so as to continue to reform and improve the teaching management of continuing medical education.,literature and information retrieval methods were used and relevant personnel was interviewed about the content,form,time and suggestions of continuing medical education.The results indicate the presence of training content is too wide,training methods are too simple,and there is lack of medical humanities quality training and other issues in continuing medical education.Variety of training methods should be increased,the quality of medical humanities education and training should be enhanced,featured training should be developed to make the continuing medical education more scientific,standardized and highly efficient.
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Objective To discuss the clinical value of improved percutaneous lung biopsy under CT guidance.Methods Reviewed the clinical data of 80 cases of improved percutaneous lung biopsy under CT guidance,which were confirmed by surgery or pathology.When took the operation,18 G semi-automatic cutting biopsy needle (made in American Cook's company) was used to improve the ways of positioning scan,two-step method of insert the needle,and the cutting technique of coaxial in each direction.Results All of the 80 patients were successfully completed pathological biopsy,successful rate was 100.0％ (80/80),and diagnosis rate was 98.8％ (79/80),incidence of complication was 10.0 ％ (8/80),all of these patients were recovered after treatment 2-5 d.Conclusion The method of improved percutaneous lung biopsy under CT guidance is safe,effective,simple and practical,which has high successful rate and low complication rate,the generalizability is certain.
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Objective To compare the clinical efficacy and safety between the improved ligation method and the traditional ligation method in the treatment of internal hemorrhoid. Methods Double blind and randomized study were made in 86 patients with internal hemorrhoid. The traditional method was performed by ligation of internal hemorrhoidal body, and the improved method was to ligate rectal mucous membrane above internal hemorrhoidal radicles. Results The improved method has better effect than the traditional method. The clinical symptoms of internal hemorrhoid such as bleeding,pain,constipation,edema,itching and erosion in the patients treated by the improved method were significantly improved compared with the patients treated by the traditional method(P